Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies
Microfluidic platforms provide a drastic enhance in throughput whereas minimizing pattern utilization and hands-on time, which make them essential instruments for large-scale organic research. A spread of such methods have been developed for enzyme exercise research, though their complexity largely hinders their software to the broader scientific group.
Right here, we current adaptation of an easy-to-use business microfluidic qPCR system for performing enzyme kinetic research. We display the performance of the Fluidigm Biomark HD system (the Fluidigm system) by figuring out the kinetic properties of three oxidases in a resorufin-based fluorescence assay. The outcomes obtained within the microfluidic system proved reproducible and akin to those obtained in a normal microplate-based assay. With a variety of easy-to-use, off-the-shelf elements, the microfluidic system presents itself as a easy and customizable platform for high-throughput enzyme exercise research.
A novel TaqMan qPCR assay for speedy detection and quantification of pro-inflammatory microalgae Prototheca spp. in milk samples
Animal or human protothecosis belongs to relatively uncommon, endemic, pro-inflammatory infections. It’s brought on by achlorophyllous algae of the genus Prototheca. Particularly, P. bovis (previously P. zopfii genotype 2) is usually inflected as a non-bacterial causative agent of dairy cattle mastitis. On this research, we current a multiplex real-time PCR (qPCR) system for speedy and precise Prototheca spp. detection and quantification. Restrict of detection, diagnostic sensitivity, and specificity have been decided.
For the primary time, particular sequences of AccD (encoding acetyl CoA reductase) for P. bovis, cox1 (encoding cytochrome C oxidase subunit 1) for P. wickerhamii, cytB (encoding cytochrome B) for P. blashkeae and atp6 (encoding transporting ATPase F0 subunit 6) for P. ciferrii (previously P. zopfii genotype 1) have been used for species identification and quantification along with 28S rRNA sequence detecting genus Prototheca. The developed qPCR assay was utilized to 55 particular person cow milk samples from a herd suspected of protothecosis, 41 bulk milk samples from completely different Czech farms, 16 boxed milk samples bought in supermarkets and 21 environmental samples originating from a farm suspected of protothecosis. Our work thus presents the chance to diagnose protothecosis within the samples, the place bacterial mastitis is essentially the most generally presumed and thereby helping ample corrective measures to be taken.
Lowered ribosomal DNA transcription within the prefrontal cortex of suicide victims: consistence of latest molecular RT-qPCR findings with earlier morphometric information from AgNOR-stained pyramidal neurons
Prefrontal cortical areas play a key function in behavioural regulation, which is profoundly disturbed in suicide. The research was carried out on frozen cortical samples from the anterior cingulate cortex (dorsal and ventral elements, ACd and ACv), the orbitofrontal cortex (OFC), and the dorsolateral cortex (DLC) obtained from 20 suicide completers (predominantly violent) with unknown psychiatric analysis and 21 non-suicidal controls. The relative degree of ribosomal RNA (rRNA) as a marker of the transcriptional exercise of ribosomal DNA (rDNA) was evaluated bilaterally in prefrontal areas talked about above (i.e. in eight areas of curiosity, ROIs) by reverse transcription and quantitative polymerase chain response (RT-qPCR).
The general statistical evaluation revealed a lower in rDNA exercise in suicide victims versus controls, notably in male topics. Additional ROI-specific submit hoc analyses revealed a major lower on this exercise in suicides in comparison with non-suicides in 5 ROIs. This impact was accentuated within the ACv, the place it was noticed bilaterally. Our findings counsel that decreased rDNA transcription within the prefrontal cortex performs an essential function in suicide pathogenesis and corresponds with our earlier morphometric analyses of AgNOR-stained neurons.
Choice and validation of inside reference genes for qPCR in Polygonatum cyrtonema tubers at completely different growth levels and in response to abiotic stress
With a purpose to analyze the expression of genes concerned in steroidal saponin biosynthesis pathway in Polygonatum cyrtonema tubers, it is rather essential to pick out inside reference genes which might be stably expressed at completely different growth levels and in response to abiotic stress. Based on the beforehand established P. cyrtonema transcriptome database and reported inside reference genes in plant, this research systematically analyzed eight candidate inside reference genes together with histone H2 A, glyceraldehyde-3-phosphate dehydrogenase, ACTIN, β-tubulin, ubiquitin-conjugating enzyme-E2-10, elongation issue 1-alpha isoform, 18 S rRNA and α-tubulin four for expression stability in P. cyrtonema tubers at completely different growth levels and in response to methyl jasmonate(MeJA) stress by utilizing Actual time fluorescence quantitative PCR(qPCR). Based mostly on the statistical evaluation of qPCR outcomes by utilizing GeNorm, NormFinder and BestKeeper softwares, the expression of ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform are essentially the most steady in P. cyrtonema tubes at completely different growth levels and in response to MeJA stress.
Green qPCR SuperMix UDG |
20-abx098032 |
Abbexa |
-
EUR 1135.00
-
EUR 453.00
-
EUR 662.00
|
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|
Top Green qPCR SuperMix |
20-abx098033 |
Abbexa |
-
EUR 829.00
-
EUR 217.00
-
EUR 1135.00
-
EUR 411.00
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|
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Tip Green qPCR SuperMix |
20-abx098034 |
Abbexa |
-
EUR 968.00
-
EUR 230.00
-
EUR 1330.00
-
EUR 467.00
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Library Quantification qPCR SuperMix |
20-abx098896 |
Abbexa |
|
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Pro QPCR SuperMix Kit - ROX premixed |
K5056200 |
Biochain |
200 reactions |
EUR 188 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit - ROX premixed |
K5056400 |
Biochain |
400 reactions |
EUR 299 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
cDNA Synthesis SuperMix for qPCR |
20-abx09801920ulSystems |
Abbexa |
|
- 100 rxns ×20 ul Systems
- 50 rxns × 20 ul Systems
|
|
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054200 |
Biochain |
200 reactions |
EUR 408 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054400 |
Biochain |
400 reactions |
EUR 594 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058200 |
Biochain |
200 reactions |
EUR 211 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058400 |
Biochain |
400 reactions |
EUR 320 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Top Green qPCR SuperMix (+Dye II) |
20-abx098890 |
Abbexa |
-
EUR 829.00
-
EUR 217.00
-
EUR 1135.00
-
EUR 411.00
|
|
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Tip Green qPCR SuperMix (+Dye II) |
20-abx098891 |
Abbexa |
-
EUR 968.00
-
EUR 230.00
-
EUR 1330.00
-
EUR 467.00
|
|
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cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 718 |
|
HiScript II Q RT SuperMix for qPCR |
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
HiScript II Q Select RT SuperMix for qPCR |
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
HS-100-QPCR |
CORNING |
100/pk |
EUR 318 |
Description: Sealing Products; Sealing films - Axygen |
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
HS-150-QPCR |
CORNING |
100/pk |
EUR 318 |
Description: Sealing Products; Sealing films - Axygen |
HiScript III RT SuperMix for qPCR (+g DNA wiper) |
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 293 |
HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 244 |
PCR SuperMix |
20-abx098887 |
Abbexa |
|
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PCR SuperMix (-dye) |
20-abx098002 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
|
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PCR SuperMix (+dye) |
20-abx098003 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 6174.00
-
EUR 258.00
|
|
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cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
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Plus PCR SuperMix |
20-abx098888 |
Abbexa |
|
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|
Library Amplification SuperMix |
20-abx098894 |
Abbexa |
|
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OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 89 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 141 |
Evo? cDNA Supermix |
M1168-100 |
Biovision |
|
EUR 381 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
|
EUR 267 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
|
EUR 441 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
|
EUR 289 |
Entrans qPCR Probe Kit |
RK21209 |
Abclonal |
40 RXN |
Ask for price |
|
qPCR Control Template Kit |
G929 |
ABM |
100 rxns |
EUR 148 |
Lentivirus qPCR Quantification Kit |
K1471-100 |
Biovision |
100 Rxns |
EUR 298 |
Retrovirus qPCR Quantification Kit |
K1472-100 |
Biovision |
100 Rxns |
EUR 318 |
QPCR Positive Control Kit |
M1126-100 |
Biovision |
|
EUR 403 |
COLLECTION CONTAINER 1L, EVA FILM |
91-200-01 |
CORNING |
1/pk |
EUR 80 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 2L, EVA FILM |
91-200-02 |
CORNING |
1/pk |
EUR 88 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
91-200-05 |
CORNING |
1/pk |
EUR 99 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-10 |
CORNING |
1/pk |
EUR 101 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-20 |
CORNING |
1/pk |
EUR 110 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-36 |
CORNING |
1/pk |
EUR 86 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-39 |
CORNING |
1/pk |
EUR 89 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 1L, EVA FILM |
91-200-42 |
CORNING |
1/pk |
EUR 73 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
91-200-43 |
CORNING |
1/pk |
EUR 151 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-45 |
CORNING |
1/pk |
EUR 126 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-47 |
CORNING |
1/pk |
EUR 132 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 50L, EVA FILM |
91-200-48 |
CORNING |
1/pk |
EUR 146 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 100L, EVA FILM |
91-200-82 |
CORNING |
1/pk |
EUR 279 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 200L, EVA FILM |
91-200-83 |
CORNING |
1/pk |
EUR 292 |
Description: Flexible Containers; Bioprocess Bags and Parts |
T PCR SuperMix (-dye) |
20-abx098005 |
Abbexa |
|
|
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T PCR SuperMix (+dye) |
20-abx098006 |
Abbexa |
|
|
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EasyPfu PCR SuperMix (-dye) |
20-abx098009 |
Abbexa |
|
|
|
FastPfu PCR SuperMix (-dye) |
20-abx098010 |
Abbexa |
|
|
|
OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 169 |
Methylamp MS-qPCR Fast Kit |
P-1028 |
EpiGentek |
200 Reactions |
EUR 496.6 |
Description: fast delivery possible |
Universal qPCR Mixture |
QPCR1000-UNIV |
Bio Basic |
4X1.25ml |
EUR 276.2 |
|
COLLECTION CONTAINER 500 ML, EVA FILM |
91-200-41 |
CORNING |
1/pk |
EUR 69 |
Description: Flexible Containers; Bioprocess Bags and Parts |
LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
RA900A-1 |
SBI |
20 profiles |
EUR 850 |
|
Frit Kit |
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
PCR SuperMix for PAGE (+dye) |
20-abx098004 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
|
|
|
Column Packing Kit |
PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
RA930A-1 |
SBI |
20 profiles |
EUR 850 |
|
PCR Mycoplasma Detection Kit |
M034-Kit |
TOKU-E |
Kit |
EUR 266 |
Coronavirus Rapid RT-qPCR Detection Kit |
K1461-100 |
Biovision |
100 Rxns |
EUR 551 |
Adeno-Associated Virus qPCR Quantification Kit |
K1473-100 |
Biovision |
100 Rxns |
EUR 348 |
Protein Eva-1 Homolog C (EVA1C) Antibody |
20-abx302696 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Entrans qPCR Probe Set |
RK21210 |
Abclonal |
40 RXN |
Ask for price |
|
Neural Lineage qPCR Profiler |
RA500A-1 |
SBI |
10 reactions |
EUR 999 |
|
Cancer MicroRNA qPCR Array |
RA610A-1 |
SBI |
10 profiles |
EUR 1172 |
|
Regulatory RNA qPCR Profiler |
RA950A-1 |
SBI |
20 rxn |
EUR 880 |
|
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055200 |
Biochain |
200 reactions |
EUR 376 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055400 |
Biochain |
400 reactions |
EUR 572 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1110 |
|
miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 732 |
|
Green Two-Step qRT-PCR SuperMix |
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 648 |
|
Green One-Step qRT-PCR SuperMix |
20-abx09803820ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Probe One-Step qRT-PCR SuperMix |
20-abx09804220ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Fly First-Strand cDNA Synthesis SuperMix |
20-abx09885820ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 456 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1453 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV100PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV105PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV120PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV125PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS700A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS720A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS740A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
CAS510A-KIT |
SBI |
1 Kit |
EUR 805 |
|
The 2 inside reference genes have been additional validated by analyzing the expression of four genes concerned in steroidal saponin biosynthesis pathways. In conclusion, ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform can be utilized as essentially the most acceptable inside reference genes for qPCR evaluation in P. cyrtonema. This research additionally present a basis for future examine the molecular mechanism of steroidal saponin biosynthesis pathways in P. cyrtonema.
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