Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies

Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies

Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies

Microfluidic platforms provide a drastic enhance in throughput whereas minimizing pattern utilization and hands-on time, which make them essential instruments for large-scale organic research. A spread of such methods have been developed for enzyme exercise research, though their complexity largely hinders their software to the broader scientific group.

Right here, we current adaptation of an easy-to-use business microfluidic qPCR system for performing enzyme kinetic research. We display the performance of the Fluidigm Biomark HD system (the Fluidigm system) by figuring out the kinetic properties of three oxidases in a resorufin-based fluorescence assay. The outcomes obtained within the microfluidic system proved reproducible and akin to those obtained in a normal microplate-based assay. With a variety of easy-to-use, off-the-shelf elements, the microfluidic system presents itself as a easy and customizable platform for high-throughput enzyme exercise research.

A novel TaqMan qPCR assay for speedy detection and quantification of pro-inflammatory microalgae Prototheca spp. in milk samples

Animal or human protothecosis belongs to relatively uncommon, endemic, pro-inflammatory infections. It’s brought on by achlorophyllous algae of the genus Prototheca. Particularly, P. bovis (previously P. zopfii genotype 2) is usually inflected as a non-bacterial causative agent of dairy cattle mastitis. On this research, we current a multiplex real-time PCR (qPCR) system for speedy and precise Prototheca spp. detection and quantification. Restrict of detection, diagnostic sensitivity, and specificity have been decided.
For the primary time, particular sequences of AccD (encoding acetyl CoA reductase) for P. bovis, cox1 (encoding cytochrome C oxidase subunit 1) for P. wickerhamii, cytB (encoding cytochrome B) for P. blashkeae and atp6 (encoding transporting ATPase F0 subunit 6) for P. ciferrii (previously P. zopfii genotype 1) have been used for species identification and quantification along with 28S rRNA sequence detecting genus Prototheca. The developed qPCR assay was utilized to 55 particular person cow milk samples from a herd suspected of protothecosis, 41 bulk milk samples from completely different Czech farms, 16 boxed milk samples bought in supermarkets and 21 environmental samples originating from a farm suspected of protothecosis. Our work thus presents the chance to diagnose protothecosis within the samples, the place bacterial mastitis is essentially the most generally presumed and thereby helping ample corrective measures to be taken.

Lowered ribosomal DNA transcription within the prefrontal cortex of suicide victims: consistence of latest molecular RT-qPCR findings with earlier morphometric information from AgNOR-stained pyramidal neurons

Prefrontal cortical areas play a key function in behavioural regulation, which is profoundly disturbed in suicide. The research was carried out on frozen cortical samples from the anterior cingulate cortex (dorsal and ventral elements, ACd and ACv), the orbitofrontal cortex (OFC), and the dorsolateral cortex (DLC) obtained from 20 suicide completers (predominantly violent) with unknown psychiatric analysis and 21 non-suicidal controls. The relative degree of ribosomal RNA (rRNA) as a marker of the transcriptional exercise of ribosomal DNA (rDNA) was evaluated bilaterally in prefrontal areas talked about above (i.e. in eight areas of curiosity, ROIs) by reverse transcription and quantitative polymerase chain response (RT-qPCR).
The general statistical evaluation revealed a lower in rDNA exercise in suicide victims versus controls, notably in male topics. Additional ROI-specific submit hoc analyses revealed a major lower on this exercise in suicides in comparison with non-suicides in 5 ROIs. This impact was accentuated within the ACv, the place it was noticed bilaterally. Our findings counsel that decreased rDNA transcription within the prefrontal cortex performs an essential function in suicide pathogenesis and corresponds with our earlier morphometric analyses of AgNOR-stained neurons.
Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies

Choice and validation of inside reference genes for qPCR in Polygonatum cyrtonema tubers at completely different growth levels and in response to abiotic stress

With a purpose to analyze the expression of genes concerned in steroidal saponin biosynthesis pathway in Polygonatum cyrtonema tubers, it is rather essential to pick out inside reference genes which might be stably expressed at completely different growth levels and in response to abiotic stress. Based on the beforehand established P. cyrtonema transcriptome database and reported inside reference genes in plant, this research systematically analyzed eight candidate inside reference genes together with histone H2 A, glyceraldehyde-3-phosphate dehydrogenase, ACTIN, β-tubulin, ubiquitin-conjugating enzyme-E2-10, elongation issue 1-alpha isoform, 18 S rRNA and α-tubulin four for expression stability in P. cyrtonema tubers at completely different growth levels and in response to methyl jasmonate(MeJA) stress by utilizing Actual time fluorescence quantitative PCR(qPCR). Based mostly on the statistical evaluation of qPCR outcomes by utilizing GeNorm, NormFinder and BestKeeper softwares, the expression of ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform are essentially the most steady in P. cyrtonema tubes at completely different growth levels and in response to MeJA stress.

Probe qPCR SuperMix

20-abx098040
  • EUR 1245.60
  • EUR 526.80
  • EUR 760.80
  • 15 ml
  • 1 ml
  • 5 ml

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1362.00
  • EUR 543.60
  • EUR 794.40
  • 15 ml
  • 1 ml
  • 5 ml

Top Green qPCR SuperMix

20-abx098033
  • EUR 994.80
  • EUR 260.40
  • EUR 1362.00
  • EUR 493.20
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix

20-abx098034
  • EUR 1161.60
  • EUR 276.00
  • EUR 1596.00
  • EUR 560.40
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Library Quantification qPCR SuperMix

20-abx098896
  • EUR 276.00
  • EUR 493.20
  • 1 ml
  • 5 ml

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054200 200 reactions
EUR 489.6
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
EUR 712.8
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054004 400 rxn
EUR 635

Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
EUR 225.6
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 358.8
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 828.00
  • EUR 678.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054002 200 rxn
EUR 418

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 253.2
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 384
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 994.80
  • EUR 260.40
  • EUR 1362.00
  • EUR 493.20
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • EUR 1161.60
  • EUR 276.00
  • EUR 1596.00
  • EUR 560.40
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 861.6

HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 282

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 282

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

HiScript III RT SuperMix for qPCR (+g DNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 351.6

PCR SuperMix

20-abx098887
  • EUR 326.40
  • EUR 693.60
  • 1 ml
  • 5 ml

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 292.8

QPCR Kit QPCR Mastermix DNA

MOL6354 EACH
EUR 231.42

PCR SuperMix (-dye)

20-abx098002
  • EUR 493.20
  • EUR 226.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 5 ml

PCR SuperMix (+dye)

20-abx098003
  • EUR 493.20
  • EUR 226.80
  • EUR 7408.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Plus PCR SuperMix

20-abx098888
  • EUR 292.80
  • EUR 560.40
  • 1 ml
  • 5 ml

Library Amplification SuperMix

20-abx098894
  • EUR 309.60
  • EUR 644.40
  • 1 ml
  • 5 ml

Evo? cDNA Supermix

M1168-100
EUR 457.2

Evo? cDNA Supermix

M1168-25
EUR 320.4

Novo? cDNA Supermix

M1169-100
EUR 529.2

Novo? cDNA Supermix

M1169-25
EUR 346.8

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 106.8

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 169.2

T PCR SuperMix (-dye)

20-abx098005
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

T PCR SuperMix (+dye)

20-abx098006
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

EasyPfu PCR SuperMix (-dye)

20-abx098009
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

FastPfu PCR SuperMix (-dye)

20-abx098010
  • EUR 292.80
  • EUR 594.00
  • 1 ml
  • 5 ml

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

COLLECTION CONTAINER 1L, EVA FILM

91-200-01 1/pk
EUR 96
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 2L, EVA FILM

91-200-02 1/pk
EUR 105.6
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-05 1/pk
EUR 118.8
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-10 1/pk
EUR 121.2
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-20 1/pk
EUR 132
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-36 1/pk
EUR 103.2
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-39 1/pk
EUR 106.8
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-43 1/pk
EUR 181.2
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-45 1/pk
EUR 151.2
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-47 1/pk
EUR 158.4
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 50L, EVA FILM

91-200-48 1/pk
EUR 175.2
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 100L, EVA FILM

91-200-82 1/pk
EUR 334.8
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 200L, EVA FILM

91-200-83 1/pk
EUR 350.4
Description: Flexible Containers; Bioprocess Bags and Parts

Collection Container 1L EVA Film

91-200-42 EACH
EUR 54.38

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

COLLECTION CONTAINER 500 ML, EVA FILM

91-200-41 1/pk
EUR 82.8
Description: Flexible Containers; Bioprocess Bags and Parts

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 493.20
  • EUR 226.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 5 ml

QPCR Positive Control Kit

M1126-100
EUR 483.6

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 357.6

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns
EUR 381.6

qPCR Control Template Kit

G929 100 rxns
EUR 177.6

Entrans qPCR Probe Kit

RK21209 40 RXN Ask for price

QPCR Kit DNA Staphylococ

MOL6370 EACH
EUR 839.04

QPCR Kit DNA Bifidobacte

MOL6418 EACH
EUR 842.46

QPCR Kit DNA Clostridium

MOL6422 EACH
EUR 876.66

QPCR Kit DNA Lactobacill

MOL6474 EACH
EUR 876.66

QPCR Kit DNA Anacardium

MOL6486 EACH
EUR 842.46

QPCR Kit DNA Bertholleti

MOL6498 EACH
EUR 876.66

QPCR Kit DNA Melanogramm

MOL6550 EACH
EUR 876.66

QPCR Kit DNA Merlangius

MOL6562 EACH
EUR 842.46

QPCR Kit DNA Phacochoeru

MOL6574 EACH
EUR 876.66

QPCR Kit DNA Pleuronecte

MOL6582 EACH
EUR 842.46

QPCR Kit DNA Pollachius

MOL6594 EACH
EUR 842.46

QPCR Kit DNA Bifidobacte

MOL6614 EACH
EUR 839.04

QPCR Kit DNA Clostridium

MOL6630 EACH
EUR 772.92

QPCR Kit DNA Enterococcu

MOL6634 EACH
EUR 839.04

QPCR Kit DNA Enterococcu

MOL6638 EACH
EUR 804.84

QPCR Kit DNA Pseudomonas

MOL6666 EACH
EUR 839.04

QPCR Kit DNA Cyclospora

MOL6694 EACH
EUR 839.04

QPCR Kit DNA Campylobact

MOL6702 EACH
EUR 839.04

QPCR Kit DNA Escherichia

MOL6710 EACH
EUR 839.04

QPCR Kit DNA Escherichia

MOL6718 EACH
EUR 1081.86

QPCR Kit DNA Enteropatho

MOL6734 EACH
EUR 839.04

QPCR Kit DNA Francisella

MOL6738 EACH
EUR 839.04

QPCR Kit DNA Mycobacteri

MOL6750 EACH
EUR 839.04

QPCR Kit DNA Shewanella

MOL6758 EACH
EUR 772.92

QPCR Kit DNA Aggregatiba

MOL6762 EACH
EUR 772.92

QPCR Kit DNA Acinetobact

MOL6766 EACH
EUR 839.04

QPCR Kit DNA Ajellomyces

MOL6770 EACH
EUR 772.92

QPCR Kit DNA Balamuthia

MOL6806 EACH
EUR 772.92

QPCR Kit DNA Bordetella

MOL6818 EACH
EUR 804.84

QPCR Kit DNA Clostridium

MOL6826 EACH
EUR 772.92

QPCR Kit DNA Clostridium

MOL6830 EACH
EUR 839.04

QPCR Kit DNA Clostridium

MOL6838 EACH
EUR 804.84

QPCR Kit DNA Cytomegalov

MOL6854 EACH
EUR 839.04

QPCR Kit DNA Enterococcu

MOL6866 EACH
EUR 772.92

QPCR Kit DNA Haemophilus

MOL6918 EACH
EUR 839.04

QPCR Kit RNA Coronavirus

MOL6946 EACH
EUR 820.8

QPCR Kit DNA Klebsiella

MOL8156 EACH
EUR 1220.94

QPCR Kit DNA Leishmania

MOL8164 EACH
EUR 839.04

QPCR Kit DNA Legionella

MOL8168 EACH
EUR 839.04

QPCR Kit DNA Legionella

MOL8176 EACH
EUR 839.04

QPCR Kit DNA Mycobacteri

MOL8192 EACH
EUR 772.92

QPCR Kit DNA Mycoplasma

MOL8204 EACH
EUR 839.04

QPCR Kit DNA Mycobacteri

MOL8212 EACH
EUR 839.04

QPCR Kit DNA Mycoplasma

MOL8216 EACH
EUR 839.04

QPCR Kit DNA Mycobacteri

MOL8220 EACH
EUR 839.04

QPCR Kit DNA Methicillin

MOL8228 EACH
EUR 839.04

QPCR Kit DNA Methicillin

MOL8232 EACH
EUR 772.92

QPCR Kit DNA Methicillin

MOL8234 EACH
EUR 982.68

QPCR Kit DNA Methicillin

MOL8236 EACH
EUR 772.92

QPCR Kit DNA Methicillin

MOL8238 EACH
EUR 982.68

QPCR Kit DNA Oxalobacter

MOL8252 EACH
EUR 804.84

QPCR Kit DNA Plasmodium

MOL8256 EACH
EUR 839.04

QPCR Kit DNA Porphyromon

MOL8260 EACH
EUR 839.04

QPCR Kit DNA Prevotella

MOL8264 EACH
EUR 772.92

QPCR Kit DNA Pneumocysti

MOL8268 EACH
EUR 839.04

QPCR Kit DNA Rickettsia

MOL8296 EACH
EUR 839.04

QPCR Kit RNA Respiratory

MOL8300 EACH
EUR 1220.94

QPCR Kit RNA Respiratory

MOL8304 EACH
EUR 1220.94

QPCR Kit RNA Respiratory

MOL8308 EACH
EUR 1220.94

QPCR Kit DNA Staphylococ

MOL8316 EACH
EUR 839.04

QPCR Kit DNA Staphylococ

MOL8320 EACH
EUR 772.92

QPCR Kit DNA Streptococc

MOL8328 EACH
EUR 772.92

QPCR Kit DNA Tannerella

MOL8356 EACH
EUR 772.92

QPCR Kit DNA Tsukamurell

MOL8360 EACH
EUR 772.92

QPCR Kit DNA Trichomonas

MOL8368 EACH
EUR 896.04

QPCR Kit DNA Mycobacteri

MOL8372 EACH
EUR 839.04

QPCR Kit DNA Trichodyspl

MOL8380 EACH
EUR 772.92

QPCR Kit DNA Ureaplasma

MOL8384 EACH
EUR 839.04

QPCR Kit DNA Blastocysti

MOL8400 EACH
EUR 772.92

QPCR Kit DNA Ancylostoma

MOL8404 EACH
EUR 772.92

QPCR Kit DNA Aspergillus

MOL8412 EACH
EUR 804.84

QPCR Kit DNA Aspergillus

MOL8424 EACH
EUR 839.04

QPCR Kit DNA AspergillusMmix

MOL8426 EACH
EUR 1069.32

QPCR Kit DNA Burkholderi

MOL8432 EACH
EUR 772.92

QPCR Kit DNA Chlamydophi

MOL8436 EACH
EUR 839.04

QPCR Kit DNA Campylobact

MOL8444 EACH
EUR 804.84

QPCR Kit DNA Chlamydiace

MOL8448 EACH
EUR 839.04

QPCR Kit DNA Cryptococcu

MOL8452 EACH
EUR 804.84

QPCR Kit DNA Chlamydophi

MOL8456 EACH
EUR 772.92

QPCR Kit DNA Chlamydophi

MOL8460 EACH
EUR 839.04

QPCR Kit DNA Cryptosporidium

MOL8464 EACH
EUR 839.04

QPCR Kit DNA Cryptosporidium

MOL8466 EACH
EUR 1069.32

QPCR Kit DNA Enterocytoz

MOL8476 EACH
EUR 840.18

QPCR Kit DNA Geosmithia

MOL8484 EACH
EUR 772.92

QPCR Kit RNA H10N8

MOL8488 EACH
EUR 788.88

QPCR Kit RNA H10N8Mmix

MOL8490 EACH
EUR 1027.14

QPCR Kit DNA Leptospirosis

MOL8500 EACH
EUR 839.04

QPCR Kit DNA LeptospirosisMmix

MOL8502 EACH
EUR 1069.32

QPCR Kit DNA Pasteurella

MOL8508 EACH
EUR 772.92

QPCR Kit DNA Streptococc

MOL8528 EACH
EUR 839.04

QPCR Kit DNA Streptococc

MOL8540 EACH
EUR 772.92

QPCR Kit DNA Streptococc

MOL8544 EACH
EUR 1450.08

QPCR Kit DNA Streptococc

MOL8548 EACH
EUR 839.04

QPCR Kit DNA Streptococc

MOL8552 EACH
EUR 772.92

QPCR Kit DNA Streptococc

MOL8556 EACH
EUR 772.92

QPCR Kit DNA Budgerigar

MOL8588 EACH
EUR 804.84

QPCR Kit DNA Bordetella

MOL8600 EACH
EUR 804.84

QPCR Kit DNA Batrachochy

MOL8616 EACH
EUR 804.84

QPCR Kit DNA Capripoxvirus

MOL8656 EACH
EUR 839.04

QPCR Kit DNA CapripoxvirusMmix

MOL8658 EACH
EUR 1069.32

QPCR Kit DNA Clavibacter

MOL8676 EACH
EUR 772.92

QPCR Kit RNA Infectious

MOL8780 EACH
EUR 839.04

QPCR Kit RNA Infectious

MOL8788 EACH
EUR 839.04

QPCR Kit DNA Mycoplasma

MOL8816 EACH
EUR 839.04

QPCR Kit DNA Mycoplasma

MOL8824 EACH
EUR 839.04

QPCR Kit DNA Mycoplasma

MOL8828 EACH
EUR 804.84

QPCR Kit DNA Mycoplasma

MOL8832 EACH
EUR 804.84

QPCR Kit DNA Ornithobact

MOL8848 EACH
EUR 1236.9

QPCR Kit DNA Trypanosoma

MOL8884 EACH
EUR 804.84

QPCR Kit DNA Tritrichomo

MOL8892 EACH
EUR 839.04

QPCR Kit DNA Trichophyto

MOL8896 EACH
EUR 804.84

QPCR Kit RNA Vesivirus2117

MOL8908 EACH
EUR 788.88

QPCR Kit RNA Vesivirus2117Mmix

MOL8910 EACH
EUR 1010.04

QPCR Kit DNA Mycoplasma

MOL8932 EACH
EUR 804.84

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 200 reactions
EUR 451.2
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 400 reactions
EUR 686.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055004 400 rxn
EUR 609

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 966

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1358.4

Protein Eva-1 Homolog C (EVA1C) Antibody

20-abx302696
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
The 2 inside reference genes have been additional validated by analyzing the expression of four genes concerned in steroidal saponin biosynthesis pathways. In conclusion, ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform can be utilized as essentially the most acceptable inside reference genes for qPCR evaluation in P. cyrtonema. This research additionally present a basis for future examine the molecular mechanism of steroidal saponin biosynthesis pathways in P. cyrtonema.

Leave a Reply

Your email address will not be published. Required fields are marked *