MicroarrayWorld Microarrays and gene expression profiling

Animal or human protothecosis belongs to relatively uncommon, endemic, pro-inflammatory infections. It’s brought on by achlorophyllous algae of the genus Prototheca. Particularly, P. bovis (previously P. zopfii genotype 2) is usually inflected as a non-bacterial causative agent of dairy cattle mastitis. On this research, we current a multiplex real-time PCR (qPCR) system for speedy and precise Prototheca spp. detection and quantification. Restrict of detection, diagnostic sensitivity, and specificity have been decided.

For the primary time, particular sequences of AccD (encoding acetyl CoA reductase) for P. bovis, cox1 (encoding cytochrome C oxidase subunit 1) for P. wickerhamii, cytB (encoding cytochrome B) for P. blashkeae and atp6 (encoding transporting ATPase F0 subunit 6) for P. ciferrii (previously P. zopfii genotype 1) have been used for species identification and quantification along with 28S rRNA sequence detecting genus Prototheca. The developed qPCR assay was utilized to 55 particular person cow milk samples from a herd suspected of protothecosis, 41 bulk milk samples from completely different Czech farms, 16 boxed milk samples bought in supermarkets and 21 environmental samples originating from a farm suspected of protothecosis. Our work thus presents the chance to diagnose protothecosis within the samples, the place bacterial mastitis is essentially the most generally presumed and thereby helping ample corrective measures to be taken.

Prefrontal cortical areas play a key function in behavioural regulation, which is profoundly disturbed in suicide. The research was carried out on frozen cortical samples from the anterior cingulate cortex (dorsal and ventral elements, ACd and ACv), the orbitofrontal cortex (OFC), and the dorsolateral cortex (DLC) obtained from 20 suicide completers (predominantly violent) with unknown psychiatric analysis and 21 non-suicidal controls. The relative degree of ribosomal RNA (rRNA) as a marker of the transcriptional exercise of ribosomal DNA (rDNA) was evaluated bilaterally in prefrontal areas talked about above (i.e. in eight areas of curiosity, ROIs) by reverse transcription and quantitative polymerase chain response (RT-qPCR).

The general statistical evaluation revealed a lower in rDNA exercise in suicide victims versus controls, notably in male topics. Additional ROI-specific submit hoc analyses revealed a major lower on this exercise in suicides in comparison with non-suicides in 5 ROIs. This impact was accentuated within the ACv, the place it was noticed bilaterally. Our findings counsel that decreased rDNA transcription within the prefrontal cortex performs an essential function in suicide pathogenesis and corresponds with our earlier morphometric analyses of AgNOR-stained neurons.

With a purpose to analyze the expression of genes concerned in steroidal saponin biosynthesis pathway in Polygonatum cyrtonema tubers, it is rather essential to pick out inside reference genes which might be stably expressed at completely different growth levels and in response to abiotic stress. Based on the beforehand established P. cyrtonema transcriptome database and reported inside reference genes in plant, this research systematically analyzed eight candidate inside reference genes together with histone H2 A, glyceraldehyde-3-phosphate dehydrogenase, ACTIN, β-tubulin, ubiquitin-conjugating enzyme-E2-10, elongation issue 1-alpha isoform, 18 S rRNA and α-tubulin four for expression stability in P. cyrtonema tubers at completely different growth levels and in response to methyl jasmonate(MeJA) stress by utilizing Actual time fluorescence quantitative PCR(qPCR). Based mostly on the statistical evaluation of qPCR outcomes by utilizing GeNorm, NormFinder and BestKeeper softwares, the expression of ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform are essentially the most steady in P. cyrtonema tubes at completely different growth levels and in response to MeJA stress.

Top Green qPCR SuperMix
20-abx098033	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Top Green qPCR SuperMix
abx098033-200l	Abbexa	200 µl	EUR 487.5

Tip Green qPCR SuperMix
abx098034-100l	Abbexa	100 µl	EUR 262.5

Tip Green qPCR SuperMix
20-abx098034	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Tip Green qPCR SuperMix
abx098034-200l	Abbexa	200 µl	EUR 437.5

Library Quantification qPCR SuperMix
abx098896-100l	Abbexa	100 µl	EUR 700

Library Quantification qPCR SuperMix
abx098896-200l	Abbexa	200 µl	EUR 962.5

Library Quantification qPCR SuperMix
20-abx098896	Abbexa	Ask for price Ask for price	1 ml 5 ml

EnTurbo™ probe qPCR SuperMix
EQ017	ELK Biotech	5mL	EUR 240

EnTurbo™ probe qPCR SuperMix
EQ017-25mL	ELK Biotech	25mL	EUR 240

EnTurbo™ probe qPCR SuperMix
EQ017-5mL	ELK Biotech	5mL	EUR 78

Top Green qPCR SuperMix (+Dye II)
abx098890-100l	Abbexa	100 µl	EUR 262.5

Top Green qPCR SuperMix (+Dye II)
20-abx098890	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Top Green qPCR SuperMix (+Dye II)
abx098890-200l	Abbexa	200 µl	EUR 487.5

Tip Green qPCR SuperMix (+Dye II)
abx098891-100l	Abbexa	100 µl	EUR 262.5

Tip Green qPCR SuperMix (+Dye II)
20-abx098891	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Tip Green qPCR SuperMix (+Dye II)
abx098891-200l	Abbexa	200 µl	EUR 437.5

EnTurbo™ SYBR Color qPCR SuperMix
EQ036	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix
EQ036-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix
EQ036-5mL	ELK Biotech	5mL	EUR 80

cDNA Synthesis SuperMix for qPCR
abx098019-100l	Abbexa	100 µl	EUR 825

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns ×20 ul Systems

cDNA Synthesis SuperMix for qPCR
abx098019-1ml	Abbexa	1 ml	EUR 2387.5

cDNA Synthesis SuperMix for qPCR
abx098019-200l	Abbexa	200 µl	EUR 1000

HyperScript™ RT SuperMix for qPCR
K1074-100	ApexBio	100 rxn (20 uL/rxn)	EUR 296
Description: Reverse Transcription & PCR|Reverse Transcription

HyperScript™ RT SuperMix for qPCR
K1074-50	ApexBio	50 rxn (20 uL/rxn)	EUR 166.4
Description: Reverse Transcription & PCR|Reverse Transcription

HiScript II Q RT SuperMix for qPCR
R222-01	Vazyme	100 rxn (20 μl/rxn)	EUR 145

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035-5mL	ELK Biotech	5mL	EUR 80

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-100l	Abbexa	100 µl	EUR 1025

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-1ml	Abbexa	1 ml	Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-200l	Abbexa	200 µl	Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 861.6

HiScript II Q Select RT SuperMix for qPCR
R232-01	Vazyme	100 rxn (20 μl/rxn)	EUR 145

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-01	Vazyme	100rxns(20µl/rxn)	EUR 326

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-01-100rxns	Vazyme	100 rxns	EUR 337.36

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-100l	Abbexa	100 µl	EUR 1012.5

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-1ml	Abbexa	1 ml	Ask for price

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-200l	Abbexa	200 µl	Ask for price

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-01	Vazyme	100 rxn (20 μl/rxn)	EUR 217.5

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-01-100rxns	Vazyme	100 rxns	EUR 225.22

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034-5mL	ELK Biotech	5mL	EUR 80

HiScript II Q RT SuperMix for qPCR (+gDNA wiper)
R223-01	Vazyme	100rxns(20µl/rxn)	EUR 159.5

HyperScript™ RT SuperMix for qPCR (with gDNA wiper)
K1174-100	ApexBio	100 rxns	EUR 320
Description: Reverse Transcription & PCR|Reverse Transcription

HyperScript™ RT SuperMix for qPCR (with gDNA wiper)
K1174-50	ApexBio	50 rxns	EUR 192
Description: Reverse Transcription & PCR|Reverse Transcription

HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper)
R233-01	Vazyme	100 rxn (20 μl/rxn)	EUR 159.5

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01	Vazyme	100 rxns (21 μl/rxn)	EUR 217.5

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01-100rxns	Vazyme	100 rxns	EUR 225.22

Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus)
11141ES10	Yeasen Biotechnology	10T	EUR 25

Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus)
11141ES60	Yeasen Biotechnology	100T	EUR 230

PCR SuperMix
20-abx098887	Abbexa	Ask for price Ask for price	1 ml 5 ml

Eva-QPCR-SuperMix-Kit
K5052200	Biochain	200 reactions	EUR 147.7

Eva-QPCR-SuperMix-Kit
K5052400	Biochain	400 reactions	EUR 247.8

2X PCR SuperMix
MB200-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

2X PCR SuperMix
MBS402025-100Reactions	MyBiosource	100Reactions	EUR 175

2X PCR SuperMix
MBS402025-4Reactions	MyBiosource	4Reactions	EUR 160

PCR SuperMix (-dye)
abx098002-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix (-dye)
20-abx098002	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

PCR SuperMix (-dye)
abx098002-200l	Abbexa	200 µl	EUR 337.5

PCR SuperMix (+dye)
abx098003-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix (+dye)
20-abx098003	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 480 ml

PCR SuperMix (+dye)
abx098003-200l	Abbexa	200 µl	EUR 337.5

Plus PCR SuperMix
abx098888-100l	Abbexa	100 µl	EUR 300

Plus PCR SuperMix
abx098888-200l	Abbexa	200 µl	EUR 612.5

Plus PCR SuperMix
20-abx098888	Abbexa	Ask for price Ask for price	1 ml 5 ml

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

Probe Direct SuperMix
abx461057-1096tests	Abbexa	10 × 96 tests	Ask for price

Probe Direct SuperMix
abx461057-596tests	Abbexa	5 × 96 tests	EUR 2800

Probe Direct SuperMix
abx461057-96tests	Abbexa	96 tests	EUR 812.5

cDNA Synthesis SuperMix
abx098014-100l	Abbexa	100 µl	EUR 725

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

cDNA Synthesis SuperMix
abx098014-1ml	Abbexa	1 ml	Ask for price

cDNA Synthesis SuperMix
abx098014-200l	Abbexa	200 µl	EUR 812.5

T PCR SuperMix (-dye)
abx098005-100l	Abbexa	100 µl	EUR 262.5

T PCR SuperMix (-dye)
abx098005-200l	Abbexa	200 µl	EUR 462.5

T PCR SuperMix (-dye)
20-abx098005	Abbexa	Ask for price Ask for price	1 ml 5 ml

T PCR SuperMix (+dye)
abx098006-100l	Abbexa	100 µl	EUR 262.5

T PCR SuperMix (+dye)
abx098006-200l	Abbexa	200 µl	EUR 462.5

T PCR SuperMix (+dye)
20-abx098006	Abbexa	Ask for price Ask for price	1 ml 5 ml

AmpHS 2X PCR SuperMix
MB203-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

EasyPfu PCR SuperMix (-dye)
20-abx098009	Abbexa	Ask for price Ask for price	1 ml 5 ml

FastPfu PCR SuperMix (-dye)
abx098010-100l	Abbexa	100 µl	EUR 300

FastPfu PCR SuperMix (-dye)
abx098010-200l	Abbexa	200 µl	EUR 575

FastPfu PCR SuperMix (-dye)
20-abx098010	Abbexa	Ask for price Ask for price	1 ml 5 ml

Library Amplification SuperMix
abx098894-100l	Abbexa	100 µl	EUR 737.5

Library Amplification SuperMix
abx098894-200l	Abbexa	200 µl	EUR 1137.5

Library Amplification SuperMix
20-abx098894	Abbexa	Ask for price Ask for price	1 ml 5 ml

OneScriptcDNA Synthesis SuperMix
G451	ABM	25 x 20 ul reactions	EUR 106.8

OneScriptcDNA Synthesis SuperMix
G452	ABM	100 x 20 ul reactions	EUR 169.2

PCR SuperMix for PAGE (+dye)
abx098004-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix for PAGE (+dye)
20-abx098004	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

PCR SuperMix for PAGE (+dye)
abx098004-200l	Abbexa	200 µl	EUR 337.5

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 202.8

OmniPCR Supermix w Fluorescent dye
MBA01-0100	Bio-Helix	100 rxns (2.5ml)	EUR 16

EnTurbo™ SYBR Green PCR SuperMix
EQ001	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix
EQ001-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix
EQ001-5mL	ELK Biotech	5mL	EUR 80

Green Two-Step qRT-PCR SuperMix
abx098035-100l	Abbexa	100 µl	EUR 925

Green Two-Step qRT-PCR SuperMix
abx098035-1ml	Abbexa	1 ml	Ask for price

Green Two-Step qRT-PCR SuperMix
abx098035-200l	Abbexa	200 µl	Ask for price

Green Two-Step qRT-PCR SuperMix
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 777.6

Green One-Step qRT-PCR SuperMix
abx098038-100l	Abbexa	100 µl	EUR 737.5

Green One-Step qRT-PCR SuperMix
20-abx09803820ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Green One-Step qRT-PCR SuperMix
abx098038-1ml	Abbexa	1 ml	Ask for price

Green One-Step qRT-PCR SuperMix
abx098038-200l	Abbexa	200 µl	EUR 1050

Probe One-Step qRT-PCR SuperMix
abx098042-100l	Abbexa	100 µl	EUR 850

Probe One-Step qRT-PCR SuperMix
20-abx09804220ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix
abx098042-1ml	Abbexa	1 ml	Ask for price

Probe One-Step qRT-PCR SuperMix
abx098042-200l	Abbexa	200 µl	EUR 1400

AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X)
MB204-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 13

Two-Step RT-PCR SuperMix (12 kb)
abx098025-100l	Abbexa	100 µl	EUR 887.5

Two-Step RT-PCR SuperMix (12 kb)
abx098025-1ml	Abbexa	1 ml	Ask for price

Two-Step RT-PCR SuperMix (12 kb)
abx098025-200l	Abbexa	200 µl	Ask for price

Two-Step RT-PCR SuperMix (12 kb)
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)
abx098026-100l	Abbexa	100 µl	EUR 1087.5

Two-Step RT-PCR SuperMix (15 kb)
abx098026-1ml	Abbexa	1 ml	Ask for price

Two-Step RT-PCR SuperMix (15 kb)
abx098026-200l	Abbexa	200 µl	Ask for price

Two-Step RT-PCR SuperMix (15 kb)
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 910.8

All-in-One cDNA Synthesis SuperMix
B24403	Bimake	200 ractions	EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408	Bimake	1000 ractions	EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

OmniPCR HS Supermix w Fluorescent dye
MBA03-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X)
MB202-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

OmniPCR FAST Supermix w Fluorescent dye
MBA02-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

OmniPCR Long Supermix w Fluorescent dye
MBA04-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

OnePCR Ultra Supermix w Fluorescent Dye
MBS402026-100Reactions	MyBiosource	100Reactions	EUR 180

OnePCR Ultra Supermix w Fluorescent Dye
MBS402026-4Reactions	MyBiosource	4Reactions	EUR 160

Fly First-Strand cDNA Synthesis SuperMix
abx098858-100l	Abbexa	100 µl	EUR 850

Fly First-Strand cDNA Synthesis SuperMix
20-abx09885820ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

Fly First-Strand cDNA Synthesis SuperMix
abx098858-1ml	Abbexa	1 ml	Ask for price

Fly First-Strand cDNA Synthesis SuperMix
abx098858-200l	Abbexa	200 µl	EUR 1037.5

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-100l	Abbexa	100 µl	EUR 875

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200l	Abbexa	200 µl	Ask for price

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-100l	Abbexa	100 µl	EUR 950

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200l	Abbexa	200 µl	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-100l	Abbexa	100 µl	EUR 1212.5

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200l	Abbexa	200 µl	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 1028.4

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-100l	Abbexa	100 µl	EUR 1212.5

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-1ml	Abbexa	1 ml	Ask for price

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-200l	Abbexa	200 µl	Ask for price

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems	Abbexa	20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)	EUR 1062

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-100l	Abbexa	100 µl	EUR 950

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-1ml	Abbexa	1 ml	Ask for price

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-200l	Abbexa	200 µl	Ask for price

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-20rxns20ulSystems	Abbexa	20 rxns × 20 ul Systems	EUR 878.4

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
abx098007-100l	Abbexa	100 µl	EUR 262.5

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
abx098007-200l	Abbexa	200 µl	EUR 475

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
20-abx098007	Abbexa	Ask for price Ask for price	1 ml 5 ml

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-100l	Abbexa	100 µl	EUR 1125

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-1ml	Abbexa	1 ml	Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-200l	Abbexa	200 µl	Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 961.2

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-100l	Abbexa	100 µl	EUR 450

Green One-Step qRT-PCR SuperMix (GC Rich)
20-abx09803920ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-1ml	Abbexa	1 ml	Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-200l	Abbexa	200 µl	EUR 1000

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-100l	Abbexa	100 µl	EUR 737.5

Probe One-Step qRT-PCR SuperMix (GC Rich)
20-abx09804120ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-1ml	Abbexa	1 ml	Ask for price

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-200l	Abbexa	200 µl	EUR 1050

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-100l	Abbexa	100 µl	EUR 850

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-1ml	Abbexa	1 ml	Ask for price

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-200l	Abbexa	200 µl	Ask for price

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 693.6

HyperScript™ First-Strand cDNA Synthesis SuperMix
K1073-100	ApexBio	100 rxn (20 uL/rxn)	EUR 297.6
Description: Reverse Transcription & PCR|Reverse Transcription

HyperScript™ First-Strand cDNA Synthesis SuperMix
K1073-50	ApexBio	50 rxn (20 uL/rxn)	EUR 166.4
Description: Reverse Transcription & PCR|Reverse Transcription

One-Step gDNA Removal and cDNA Synthesis SuperMix
abx098859-100l	Abbexa	100 µl	EUR 1062.5

One-Step gDNA Removal and cDNA Synthesis SuperMix
20-abx09885920ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix
abx098859-1ml	Abbexa	1 ml	Ask for price

One-Step gDNA Removal and cDNA Synthesis SuperMix
abx098859-200l	Abbexa	200 µl	EUR 1425

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013-5mL	ELK Biotech	5mL	EUR 80

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
abx098022-100l	Abbexa	100 µl	EUR 1012.5

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
20-abx09802220ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
abx098022-1ml	Abbexa	1 ml	Ask for price

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
abx098022-200l	Abbexa	200 µl	EUR 1325

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
abx098016-100l	Abbexa	100 µl	EUR 775

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
abx098016-1ml	Abbexa	1 ml	Ask for price

The 2 inside reference genes have been additional validated by analyzing the expression of four genes concerned in steroidal saponin biosynthesis pathways. In conclusion, ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform can be utilized as essentially the most acceptable inside reference genes for qPCR evaluation in P. cyrtonema. This research additionally present a basis for future examine the molecular mechanism of steroidal saponin biosynthesis pathways in P. cyrtonema.