MicroarrayWorld Microarrays and gene expression profiling

Animal or human protothecosis belongs to relatively uncommon, endemic, pro-inflammatory infections. It’s brought on by achlorophyllous algae of the genus Prototheca. Particularly, P. bovis (previously P. zopfii genotype 2) is usually inflected as a non-bacterial causative agent of dairy cattle mastitis. On this research, we current a multiplex real-time PCR (qPCR) system for speedy and precise Prototheca spp. detection and quantification. Restrict of detection, diagnostic sensitivity, and specificity have been decided.

For the primary time, particular sequences of AccD (encoding acetyl CoA reductase) for P. bovis, cox1 (encoding cytochrome C oxidase subunit 1) for P. wickerhamii, cytB (encoding cytochrome B) for P. blashkeae and atp6 (encoding transporting ATPase F0 subunit 6) for P. ciferrii (previously P. zopfii genotype 1) have been used for species identification and quantification along with 28S rRNA sequence detecting genus Prototheca. The developed qPCR assay was utilized to 55 particular person cow milk samples from a herd suspected of protothecosis, 41 bulk milk samples from completely different Czech farms, 16 boxed milk samples bought in supermarkets and 21 environmental samples originating from a farm suspected of protothecosis. Our work thus presents the chance to diagnose protothecosis within the samples, the place bacterial mastitis is essentially the most generally presumed and thereby helping ample corrective measures to be taken.

Prefrontal cortical areas play a key function in behavioural regulation, which is profoundly disturbed in suicide. The research was carried out on frozen cortical samples from the anterior cingulate cortex (dorsal and ventral elements, ACd and ACv), the orbitofrontal cortex (OFC), and the dorsolateral cortex (DLC) obtained from 20 suicide completers (predominantly violent) with unknown psychiatric analysis and 21 non-suicidal controls. The relative degree of ribosomal RNA (rRNA) as a marker of the transcriptional exercise of ribosomal DNA (rDNA) was evaluated bilaterally in prefrontal areas talked about above (i.e. in eight areas of curiosity, ROIs) by reverse transcription and quantitative polymerase chain response (RT-qPCR).

The general statistical evaluation revealed a lower in rDNA exercise in suicide victims versus controls, notably in male topics. Additional ROI-specific submit hoc analyses revealed a major lower on this exercise in suicides in comparison with non-suicides in 5 ROIs. This impact was accentuated within the ACv, the place it was noticed bilaterally. Our findings counsel that decreased rDNA transcription within the prefrontal cortex performs an essential function in suicide pathogenesis and corresponds with our earlier morphometric analyses of AgNOR-stained neurons.

With a purpose to analyze the expression of genes concerned in steroidal saponin biosynthesis pathway in Polygonatum cyrtonema tubers, it is rather essential to pick out inside reference genes which might be stably expressed at completely different growth levels and in response to abiotic stress. Based on the beforehand established P. cyrtonema transcriptome database and reported inside reference genes in plant, this research systematically analyzed eight candidate inside reference genes together with histone H2 A, glyceraldehyde-3-phosphate dehydrogenase, ACTIN, β-tubulin, ubiquitin-conjugating enzyme-E2-10, elongation issue 1-alpha isoform, 18 S rRNA and α-tubulin four for expression stability in P. cyrtonema tubers at completely different growth levels and in response to methyl jasmonate(MeJA) stress by utilizing Actual time fluorescence quantitative PCR(qPCR). Based mostly on the statistical evaluation of qPCR outcomes by utilizing GeNorm, NormFinder and BestKeeper softwares, the expression of ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform are essentially the most steady in P. cyrtonema tubes at completely different growth levels and in response to MeJA stress.

Pro QPCR SuperMix Kit
K5053200	Biochain	200 reactions	EUR 188
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Pro QPCR SuperMix Kit
K5053400	Biochain	400 reactions	EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Green qPCR SuperMix
20-abx098031	Abbexa	EUR 1038.00 EUR 439.00 EUR 634.00	15 ml 1 ml 5 ml
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Probe qPCR SuperMix
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Green qPCR SuperMix UDG
20-abx098032	Abbexa	EUR 1135.00 EUR 453.00 EUR 662.00	15 ml 1 ml 5 ml
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Top Green qPCR SuperMix
20-abx098033	Abbexa	EUR 829.00 EUR 217.00 EUR 1135.00 EUR 411.00	15 ml 1 ml 25 ml 5 ml
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Tip Green qPCR SuperMix
20-abx098034	Abbexa	EUR 968.00 EUR 230.00 EUR 1330.00 EUR 467.00	15 ml 1 ml 25 ml 5 ml
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Library Quantification qPCR SuperMix
20-abx098896	Abbexa	EUR 230.00 EUR 411.00	1 ml 5 ml
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Pro QPCR SuperMix Kit - ROX premixed
K5056200	Biochain	200 reactions	EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit - ROX premixed
K5056400	Biochain	400 reactions	EUR 299
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cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	EUR 690.00 EUR 565.00	100 rxns ×20 ul Systems 50 rxns × 20 ul Systems
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QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054200	Biochain	200 reactions	EUR 408
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QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054400	Biochain	400 reactions	EUR 594
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Fast Pro QPCR SuperMix Kit - ROX premixed
K5058200	Biochain	200 reactions	EUR 211
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Fast Pro QPCR SuperMix Kit - ROX premixed
K5058400	Biochain	400 reactions	EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Top Green qPCR SuperMix (+Dye II)
20-abx098890	Abbexa	EUR 829.00 EUR 217.00 EUR 1135.00 EUR 411.00	15 ml 1 ml 25 ml 5 ml
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Tip Green qPCR SuperMix (+Dye II)
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cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 718
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HiScript II Q RT SuperMix for qPCR
R222-01	Vazyme	100 rxn (20 μl/rxn)	EUR 235

HiScript II Q Select RT SuperMix for qPCR
R232-01	Vazyme	100 rxn (20 μl/rxn)	EUR 235

HiScript III RT SuperMix for qPCR (+g DNA wiper)
R323-01	Vazyme	100 rxn (20 μl/rxn)	EUR 293

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500
HS-100-QPCR	CORNING	100/pk	EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500
HS-150-QPCR	CORNING	100/pk	EUR 318
Description: Sealing Products; Sealing films - Axygen

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)
R233-01	Vazyme	100 rxn (20 μl/rxn)	EUR 244

PCR SuperMix
20-abx098887	Abbexa	EUR 272.00 EUR 578.00	1 ml 5 ml
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PCR SuperMix (-dye)
20-abx098002	Abbexa	EUR 411.00 EUR 189.00 EUR 258.00	15 ml 1 ml 5 ml
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20-abx098003	Abbexa	EUR 411.00 EUR 189.00 EUR 6174.00 EUR 258.00	15 ml 1 ml 480 ml 5 ml
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cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 565.00 EUR 481.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems
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Plus PCR SuperMix
20-abx098888	Abbexa	EUR 244.00 EUR 467.00	1 ml 5 ml
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Library Amplification SuperMix
20-abx098894	Abbexa	EUR 258.00 EUR 537.00	1 ml 5 ml
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OneScriptcDNA Synthesis SuperMix
G451	ABM	25 x 20 ul reactions	EUR 89

OneScriptcDNA Synthesis SuperMix
G452	ABM	100 x 20 ul reactions	EUR 141

Evo? cDNA Supermix
M1168-100	Biovision		EUR 381

Evo? cDNA Supermix
M1168-25	Biovision		EUR 267

Novo? cDNA Supermix
M1169-100	Biovision		EUR 441

Novo? cDNA Supermix
M1169-25	Biovision		EUR 289

qPCR Control Template Kit
G929	ABM	100 rxns	EUR 148

QPCR Positive Control Kit
M1126-100	Biovision		EUR 403

Lentivirus qPCR Quantification Kit
K1471-100	Biovision	100 Rxns	EUR 298

Retrovirus qPCR Quantification Kit
K1472-100	Biovision	100 Rxns	Ask for price

COLLECTION CONTAINER 1L, EVA FILM
91-200-01	CORNING	1/pk	EUR 80
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 2L, EVA FILM
91-200-02	CORNING	1/pk	EUR 88
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91-200-05	CORNING	1/pk	EUR 99
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91-200-10	CORNING	1/pk	EUR 101
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91-200-20	CORNING	1/pk	EUR 110
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91-200-36	CORNING	1/pk	EUR 86
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91-200-39	CORNING	1/pk	EUR 89
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91-200-42	CORNING	1/pk	EUR 73
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91-200-43	CORNING	1/pk	EUR 151
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91-200-45	CORNING	1/pk	EUR 126
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91-200-47	CORNING	1/pk	EUR 132
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COLLECTION CONTAINER 50L, EVA FILM
91-200-48	CORNING	1/pk	EUR 146
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91-200-82	CORNING	1/pk	EUR 279
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 200L, EVA FILM
91-200-83	CORNING	1/pk	EUR 292
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T PCR SuperMix (-dye)
20-abx098005	Abbexa	EUR 203.00 EUR 342.00	1 ml 5 ml
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T PCR SuperMix (+dye)
20-abx098006	Abbexa	EUR 203.00 EUR 342.00	1 ml 5 ml
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EasyPfu PCR SuperMix (-dye)
20-abx098009	Abbexa	EUR 203.00 EUR 342.00	1 ml 5 ml
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FastPfu PCR SuperMix (-dye)
20-abx098010	Abbexa	EUR 244.00 EUR 495.00	1 ml 5 ml
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OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 97

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 169

Methylamp MS-qPCR Fast Kit
P-1028	EpiGentek	200 Reactions	EUR 496.6
Description: fast delivery possible

Universal qPCR Mixture
QPCR1000-UNIV	Bio Basic	4X1.25ml	EUR 276.2
Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

COLLECTION CONTAINER 500 ML, EVA FILM
91-200-41	CORNING	1/pk	EUR 69
Description: Flexible Containers; Bioprocess Bags and Parts

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA900A-1	SBI	20 profiles	EUR 850
Category: Long Non-coding RNA Tools

Column Packing Kit
PACK-KIT	Next Advance	1pack	EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 266

PCR SuperMix for PAGE (+dye)
20-abx098004	Abbexa	EUR 411.00 EUR 189.00 EUR 258.00	15 ml 1 ml 5 ml
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Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA930A-1	SBI	20 profiles	EUR 850
Category: Long Non-coding RNA Tools

igScript RT-qPCR Kit - 50 Reactions
4513	Intact Genomics	1/EA	EUR 248

igScript RT-qPCR Kit - 200 Reactions
4515	Intact Genomics	1/EA	EUR 716

Coronavirus Rapid RT-qPCR Detection Kit
K1461-100	Biovision	100 Rxns	EUR 551

Adeno-Associated Virus qPCR Quantification Kit
K1473-100	Biovision	100 Rxns	EUR 348

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT	SBI	1 kit (10 rxn)	EUR 1110
Category: Cas9

Protein Eva-1 Homolog C (EVA1C) Antibody
20-abx302696	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
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QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055200	Biochain	200 reactions	EUR 376
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055400	Biochain	400 reactions	EUR 572
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Neural Lineage qPCR Profiler
RA500A-1	SBI	10 reactions	EUR 999
Category: Lineage Profilers

Cancer MicroRNA qPCR Array
RA610A-1	SBI	10 profiles	EUR 1172
Category: MicroRNA Tools

Regulatory RNA qPCR Profiler
RA950A-1	SBI	20 rxn	EUR 880
Category: Long Non-coding RNA Tools

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT	SBI	1 Kit	EUR 805
Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

All-in-One cDNA Synthesis SuperMix
B24403	Bimake	200 ractions	EUR 456
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408	Bimake	1000 ractions	EUR 1453
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-20rxns20ulSystems	Abbexa	20 rxns × 20 ul Systems	EUR 732
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Green Two-Step qRT-PCR SuperMix
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 648
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Green One-Step qRT-PCR SuperMix
20-abx09803820ulSystems	Abbexa	EUR 495.00 EUR 746.00	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems
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Probe One-Step qRT-PCR SuperMix
20-abx09804220ulSystems	Abbexa	EUR 592.00 EUR 1024.00	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems
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Fly First-Strand cDNA Synthesis SuperMix
20-abx09885820ulSystems	Abbexa	EUR 718.00 EUR 578.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems
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Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS750A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS770A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS790A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
CAS9LIG-KIT	SBI	1 Kit	EUR 153
Category: Cas9

One.Step RT-qPCR Probe Kit (2X concentrated)
105-500	GeneOn	2 x 1,25 ml	EUR 458

One.Step RT-qPCR Probe Kit (2X concentrated)
105-502	GeneOn	10 x 1,25 ml	EUR 1719

piggyBac qPCR copy number kit, 20 reactions
PBC100A-1	SBI	20 reactions	EUR 331
Category: PiggyBac Technology

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN320A-KIT	SBI	1 Kit	EUR 4941
Category: PinPoint Integrase Tools

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN340iPS-KIT	SBI	1 Kit	EUR 4941
Category: PinPoint Integrase Tools

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles
RA910A-1	SBI	20 profiles	EUR 1108
Category: Long Non-coding RNA Tools

HotTaq Probe qPCR Mix (ROX)
BT11001	Bioatlas	250rxn	EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)
BT11003	Bioatlas	250rxn	EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix
BT11004	Bioatlas	250rxn	EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)
BT11101	Bioatlas	250rxn	EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)
BT11103	Bioatlas	250rxn	EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix
BT11104	Bioatlas	250rxn	EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

SYBR Green qPCR Master Mix
HY-K0501	MedChemExpress	5 mL (500 rxns)	EUR 263

RT Master Mix for qPCR
HY-K0510	MedChemExpress	1 mL (100 rxns)	EUR 291

BrightGreen 5X qPCR MasterMix-iCycler
MasterMix-5C	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-ROX
MasterMix-5P	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-iCycler
MasterMix-5PC	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-Multiplex
MasterMix-5PM	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

BrightGreen 5X qPCR MasterMix-ROX
MasterMix-5R	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

BrightGreen 2X qPCR MasterMix-iCycler
MasterMix-iC	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-iCycler
MasterMix-iC-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 376

KiloGreen 2X qPCR MasterMix-iCycler
MasterMix-KC	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 140

KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler
MasterMix-mC	ABM	4 x 1.25 ml for 500 reactions	EUR 151

BrightGreen miRNA qPCR MasterMix-ROX
MasterMix-mR	ABM	4 x 1.25 ml for 500 reactions	EUR 151

TaqProbe 2X qPCR MasterMix-ROX
MasterMix-P	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

TaqProbe 2X qPCR MasterMix-iCycler
MasterMix-PC	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex
MasterMix-PM	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 376

AceQ qPCR Probe Master Mix
Q112-02	Vazyme	500 rxn (20 μl/rxn)	EUR 221

AceQ qPCR Probe Master Mix
Q112-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 646

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE620A-KIT	SBI	1 kit	EUR 2132
Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE622A-KIT	SBI	1 kit	EUR 2132
Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE624A-KIT	SBI	1 kit	EUR 2132
Category: Gene Editing

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 578
Shipped within 5-10 working days.

Two-Step RT-PCR SuperMix (12 kb)
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 606
Shipped within 5-10 working days.

Two-Step RT-PCR SuperMix (15 kb)
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 759
Shipped within 5-10 working days.

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems	Abbexa	20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)	EUR 885
Shipped within 5-10 working days.

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)
105-520	GeneOn	2 x 1,25 ml	EUR 458

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)
105-522	GeneOn	10 x 1,25 ml	EUR 1719

igScript One Step RT-qPCR Kit - 100 Reactions
4214	Intact Genomics	1/EA	EUR 253

igScript One Step RT-qPCR Kit - 500 Reactions
4218	Intact Genomics	1/EA	EUR 773

vWF Acty. Kit
ABP-ACT-KIT	Abpcorp	12 x 8 microwells	EUR 428

vWF Ant. Kit
ABP-TOT-KIT	Abpcorp	12 x 8 microwells	EUR 394

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)
CAS620A-KIT	SBI	1 kit	EUR 2152
Category: Cas9
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN300A-KIT	SBI	1 Kit	EUR 2798
Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN400A-KIT	SBI	1 Kit	EUR 2798
Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN410A-KIT	SBI	1 Kit	EUR 4335
Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN412A-KIT	SBI	1 Kit	EUR 4335
Category: PinPoint Integrase Tools

2x SYBR Green qPCR Master Mix
B21202	Bimake	5 mL	EUR 224
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2x SYBR Green qPCR Master Mix
B21203	Bimake	25 mL	EUR 856
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

HotTaq Probe qPCR Mix (no ROX)
BT11002	Bioatlas	250rxn	EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)
BT11102	Bioatlas	250rxn	EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Universal miRNA qPCR Primer (10 µM)
abx298006-200ul	Abbexa	200 ul	EUR 189
Shipped within 5-10 working days.

Opti-Seal qPCR and Storage Films
G495	ABM	100 Films/Box	EUR 202

Opti-Seal qPCR 384 Well Plate
G935	ABM	10 Plates/Box	EUR 89

Opti-Seal qPCR 384 Well Plate
G936	ABM	100 Plates/Case	EUR 428

BrightGreen 5X qPCR MasterMix-Low ROX
MasterMix-5L	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-Low ROX
MasterMix-5PL	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-No Dye
MasterMix-5PS	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

BrightGreen 5X qPCR MasterMix-No Dye
MasterMix-5S	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

BrightGreen Express 2X qPCR MasterMix-iCycler
MasterMix-EC	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen Express 2X qPCR MasterMix-ROX
MasterMix-ER	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 140

KiloGreen 2X qPCR MasterMix-No Dye
MasterMix-KS	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 376

BrightGreen miRNA qPCR MasterMix-Low ROX
MasterMix-mL	ABM	4 x 1.25 ml for 500 reactions	EUR 151

BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS	ABM	4 x 1.25 ml for 500 reactions	EUR 151

TaqProbe 2X qPCR MasterMix-Low ROX
MasterMix-PL	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

TaqProbe 2X qPCR MasterMix-No Dye
MasterMix-PS	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 376

AceQ Universal SYBR qPCR Master Mix
Q511-02	Vazyme	500 rxn (20 μl/rxn)	EUR 221

AceQ Universal SYBR qPCR Master Mix
Q511-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 646

ChamQ Universal SYBR qPCR Master Mix
Q711-02	Vazyme	500 rxn (20 μl/rxn)	EUR 221

ChamQ Universal SYBR qPCR Master Mix
Q711-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 646

PrecisionX Multiplex gRNA Cloning Kit
CAS9-GRNA-KIT	SBI	10 rxn	EUR 445
Category: Cas9

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
20-abx098007	Abbexa	EUR 217.00 EUR 356.00	1 ml 5 ml
Shipped within 5-10 working days.

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 606
Shipped within 5-10 working days.

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 662
Shipped within 5-10 working days.

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 857
Shipped within 5-10 working days.

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 801
Shipped within 5-10 working days.

Green One-Step qRT-PCR SuperMix (GC Rich)
20-abx09803920ulSystems	Abbexa	EUR 592.00 EUR 1024.00	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems
Shipped within 5-10 working days.

Probe One-Step qRT-PCR SuperMix (GC Rich)
20-abx09804120ulSystems	Abbexa	EUR 495.00 EUR 746.00	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems
Shipped within 5-10 working days.

One-Step gDNA Removal and cDNA Synthesis SuperMix
20-abx09885920ulSystems	Abbexa	EUR 1024.00 EUR 746.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems
Shipped within 5-10 working days.

mRNAExpress mRNA Synthesis kit (5 reactions)
MR-KIT-1	SBI	5 reactions	EUR 1152
Category: Stem Cell Products

One.Step RT-qPCR Probe Kit with HighROX (2X concentrated)
105-510	GeneOn	2 x 1,25 ml	EUR 440

One.Step RT-qPCR Probe Kit with HighROX (2X concentrated)
105-512	GeneOn	10 x 1,25 ml	EUR 1628

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
20-abx09802220ulSystems	Abbexa	EUR 940.00 EUR 690.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems
Shipped within 5-10 working days.

ROX reference dye for qPCR, 500 uL
31110	lumiprobe	500 uL	EUR 46

ROX reference dye for qPCR, 1 mL
41110	lumiprobe	1 mL	EUR 52

SYBR Green qPCR Master Mix (High ROX)
HY-K0521	MedChemExpress	1 mL (100 rxns)	EUR 113

SYBR Green qPCR Master Mix (Low ROX)
HY-K0522	MedChemExpress	5 mL (500 rxns )	EUR 257

SYBR Green qPCR Master Mix (No ROX)
HY-K0523	MedChemExpress	5 mL (500 rxns )	EUR 257

Opti-Seal qPCR Semi-skirted plate (Standard)
G908	ABM	10 Plates/Bag	EUR 76

Opti-Seal qPCR Semi-skirted plate (Standard)
G911	ABM	100 Plates/Case	EUR 302

The 2 inside reference genes have been additional validated by analyzing the expression of four genes concerned in steroidal saponin biosynthesis pathways. In conclusion, ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform can be utilized as essentially the most acceptable inside reference genes for qPCR evaluation in P. cyrtonema. This research additionally present a basis for future examine the molecular mechanism of steroidal saponin biosynthesis pathways in P. cyrtonema.