Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies

Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies

Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies

Microfluidic platforms provide a drastic enhance in throughput whereas minimizing pattern utilization and hands-on time, which make them essential instruments for large-scale organic research. A spread of such methods have been developed for enzyme exercise research, though their complexity largely hinders their software to the broader scientific group.

Right here, we current adaptation of an easy-to-use business microfluidic qPCR system for performing enzyme kinetic research. We display the performance of the Fluidigm Biomark HD system (the Fluidigm system) by figuring out the kinetic properties of three oxidases in a resorufin-based fluorescence assay. The outcomes obtained within the microfluidic system proved reproducible and akin to those obtained in a normal microplate-based assay. With a variety of easy-to-use, off-the-shelf elements, the microfluidic system presents itself as a easy and customizable platform for high-throughput enzyme exercise research.

A novel TaqMan qPCR assay for speedy detection and quantification of pro-inflammatory microalgae Prototheca spp. in milk samples

Animal or human protothecosis belongs to relatively uncommon, endemic, pro-inflammatory infections. It’s brought on by achlorophyllous algae of the genus Prototheca. Particularly, P. bovis (previously P. zopfii genotype 2) is usually inflected as a non-bacterial causative agent of dairy cattle mastitis. On this research, we current a multiplex real-time PCR (qPCR) system for speedy and precise Prototheca spp. detection and quantification. Restrict of detection, diagnostic sensitivity, and specificity have been decided.
For the primary time, particular sequences of AccD (encoding acetyl CoA reductase) for P. bovis, cox1 (encoding cytochrome C oxidase subunit 1) for P. wickerhamii, cytB (encoding cytochrome B) for P. blashkeae and atp6 (encoding transporting ATPase F0 subunit 6) for P. ciferrii (previously P. zopfii genotype 1) have been used for species identification and quantification along with 28S rRNA sequence detecting genus Prototheca. The developed qPCR assay was utilized to 55 particular person cow milk samples from a herd suspected of protothecosis, 41 bulk milk samples from completely different Czech farms, 16 boxed milk samples bought in supermarkets and 21 environmental samples originating from a farm suspected of protothecosis. Our work thus presents the chance to diagnose protothecosis within the samples, the place bacterial mastitis is essentially the most generally presumed and thereby helping ample corrective measures to be taken.

Lowered ribosomal DNA transcription within the prefrontal cortex of suicide victims: consistence of latest molecular RT-qPCR findings with earlier morphometric information from AgNOR-stained pyramidal neurons

Prefrontal cortical areas play a key function in behavioural regulation, which is profoundly disturbed in suicide. The research was carried out on frozen cortical samples from the anterior cingulate cortex (dorsal and ventral elements, ACd and ACv), the orbitofrontal cortex (OFC), and the dorsolateral cortex (DLC) obtained from 20 suicide completers (predominantly violent) with unknown psychiatric analysis and 21 non-suicidal controls. The relative degree of ribosomal RNA (rRNA) as a marker of the transcriptional exercise of ribosomal DNA (rDNA) was evaluated bilaterally in prefrontal areas talked about above (i.e. in eight areas of curiosity, ROIs) by reverse transcription and quantitative polymerase chain response (RT-qPCR).
The general statistical evaluation revealed a lower in rDNA exercise in suicide victims versus controls, notably in male topics. Additional ROI-specific submit hoc analyses revealed a major lower on this exercise in suicides in comparison with non-suicides in 5 ROIs. This impact was accentuated within the ACv, the place it was noticed bilaterally. Our findings counsel that decreased rDNA transcription within the prefrontal cortex performs an essential function in suicide pathogenesis and corresponds with our earlier morphometric analyses of AgNOR-stained neurons.
Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies

Choice and validation of inside reference genes for qPCR in Polygonatum cyrtonema tubers at completely different growth levels and in response to abiotic stress

With a purpose to analyze the expression of genes concerned in steroidal saponin biosynthesis pathway in Polygonatum cyrtonema tubers, it is rather essential to pick out inside reference genes which might be stably expressed at completely different growth levels and in response to abiotic stress. Based on the beforehand established P. cyrtonema transcriptome database and reported inside reference genes in plant, this research systematically analyzed eight candidate inside reference genes together with histone H2 A, glyceraldehyde-3-phosphate dehydrogenase, ACTIN, β-tubulin, ubiquitin-conjugating enzyme-E2-10, elongation issue 1-alpha isoform, 18 S rRNA and α-tubulin four for expression stability in P. cyrtonema tubers at completely different growth levels and in response to methyl jasmonate(MeJA) stress by utilizing Actual time fluorescence quantitative PCR(qPCR). Based mostly on the statistical evaluation of qPCR outcomes by utilizing GeNorm, NormFinder and BestKeeper softwares, the expression of ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform are essentially the most steady in P. cyrtonema tubes at completely different growth levels and in response to MeJA stress.

Probe qPCR SuperMix

20-abx098040
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 5 ml

Green qPCR SuperMix

abx098031-100l 100 µl
EUR 675

Green qPCR SuperMix

abx098031-200l 200 µl
EUR 912.5

Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
EUR 180

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 326

Green qPCR SuperMix UDG

20-abx098032
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 5 ml

Top Green qPCR SuperMix

20-abx098033
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix

20-abx098034
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Green qPCR SuperMix UDG

abx098032-100l 100 µl
EUR 687.5

Green qPCR SuperMix UDG

abx098032-200l 200 µl
EUR 912.5

Top Green qPCR SuperMix

abx098033-100l 100 µl
EUR 262.5

Top Green qPCR SuperMix

abx098033-200l 200 µl
EUR 487.5

Tip Green qPCR SuperMix

abx098034-100l 100 µl
EUR 262.5

Tip Green qPCR SuperMix

abx098034-200l 200 µl
EUR 437.5

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 211

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 354

Library Quantification qPCR SuperMix

20-abx098896
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

Library Quantification qPCR SuperMix

abx098896-100l 100 µl
EUR 700

Library Quantification qPCR SuperMix

abx098896-200l 200 µl
EUR 962.5

EnTurbo™ probe qPCR SuperMix

EQ017 5mL
EUR 240

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Top Green qPCR SuperMix (+Dye II)

abx098890-100l 100 µl
EUR 262.5

Top Green qPCR SuperMix (+Dye II)

abx098890-200l 200 µl
EUR 487.5

Tip Green qPCR SuperMix (+Dye II)

abx098891-100l 100 µl
EUR 262.5

Tip Green qPCR SuperMix (+Dye II)

abx098891-200l 200 µl
EUR 437.5

EnTurbo™ SYBR Color qPCR SuperMix

EQ036 5mL
EUR 340

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

HyperScript RT SuperMix for qPCR

K1074-100 100 rxn (20 uL/rxn)
EUR 280
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR

K1074-50 50 rxn (20 uL/rxn)
EUR 150
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

cDNA Synthesis SuperMix for qPCR

abx098019-100l 100 µl
EUR 825

cDNA Synthesis SuperMix for qPCR

abx098019-1ml 1 ml
EUR 2387.5

cDNA Synthesis SuperMix for qPCR

abx098019-200l 200 µl
EUR 1000

HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 145

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)

EQ035 5mL
EUR 340

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 861.6

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-100l 100 µl
EUR 1025

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-1ml 1 ml Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-200l 200 µl Ask for price

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 145

HiScript IV RT SuperMix for qPCR (+gDNA wiper)

R423-01 100rxns(20µl/rxn)
EUR 326

HiScript III RT SuperMix for qPCR (+gDNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 217.5

Uni Cell to cDNA Synthesis SuperMix for qPCR

abx098860-100l 100 µl
EUR 1012.5

Uni Cell to cDNA Synthesis SuperMix for qPCR

abx098860-1ml 1 ml Ask for price

Uni Cell to cDNA Synthesis SuperMix for qPCR

abx098860-200l 200 µl Ask for price

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)

EQ033 5mL
EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)

EQ034 5mL
EUR 340

HyperScript RT SuperMix for qPCR (with gDNA wiper)

K1174-100 100 rxns
EUR 304
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR (with gDNA wiper)

K1174-50 50 rxns
EUR 176
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HiScript II Q RT SuperMix for qPCR (+gDNA wiper)

R223-01 100rxns(20µl/rxn)
EUR 159.5

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054200 200 reactions
EUR 468

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
EUR 711

HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 159.5

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054002 200 rxn
EUR 468

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054004 400 rxn
EUR 711

HiScript III All-in-one RT SuperMix Perfect for qPCR

R333-01 100 rxns (21 μl/rxn)
EUR 217.5

PCR SuperMix

20-abx098887
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

2X PCR SuperMix

MB200-P100 100 rxns2 x 1.25 mL
EUR 11

PCR SuperMix (-dye)

20-abx098002
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 5 ml

PCR SuperMix (+dye)

20-abx098003
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

PCR SuperMix (-dye)

abx098002-100l 100 µl
EUR 237.5

PCR SuperMix (-dye)

abx098002-200l 200 µl
EUR 337.5

PCR SuperMix (+dye)

abx098003-100l 100 µl
EUR 237.5

PCR SuperMix (+dye)

abx098003-200l 200 µl
EUR 337.5

Plus PCR SuperMix

20-abx098888
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

Evo? cDNA Supermix

M1168-100 each
EUR 457.2

Evo? cDNA Supermix

M1168-25 each
EUR 320.4

Plus PCR SuperMix

abx098888-100l 100 µl
EUR 300

Plus PCR SuperMix

abx098888-200l 200 µl
EUR 612.5

Novo? cDNA Supermix

M1169-100 each
EUR 529.2

Novo? cDNA Supermix

M1169-25 each
EUR 346.8

Probe Direct SuperMix

abx461057-1096tests 10 × 96 tests Ask for price

Probe Direct SuperMix

abx461057-596tests 5 × 96 tests
EUR 2800

Probe Direct SuperMix

abx461057-96tests 96 tests
EUR 812.5

T PCR SuperMix (-dye)

20-abx098005
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

T PCR SuperMix (+dye)

20-abx098006
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

T PCR SuperMix (-dye)

abx098005-100l 100 µl
EUR 262.5

T PCR SuperMix (-dye)

abx098005-200l 200 µl
EUR 462.5

T PCR SuperMix (+dye)

abx098006-100l 100 µl
EUR 262.5

T PCR SuperMix (+dye)

abx098006-200l 200 µl
EUR 462.5

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA Synthesis SuperMix

abx098014-100l 100 µl
EUR 725

cDNA Synthesis SuperMix

abx098014-1ml 1 ml Ask for price

cDNA Synthesis SuperMix

abx098014-200l 200 µl
EUR 812.5

AmpHS 2X PCR SuperMix

MB203-P100 100 rxns2 x 1.25 mL
EUR 11

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 200 reactions
EUR 427

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 400 reactions
EUR 682

EasyPfu PCR SuperMix (-dye)

20-abx098009
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

FastPfu PCR SuperMix (-dye)

20-abx098010
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

FastPfu PCR SuperMix (-dye)

abx098010-100l 100 µl
EUR 300

FastPfu PCR SuperMix (-dye)

abx098010-200l 200 µl
EUR 575

Library Amplification SuperMix

20-abx098894
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

Library Amplification SuperMix

abx098894-100l 100 µl
EUR 737.5

Library Amplification SuperMix

abx098894-200l 200 µl
EUR 1137.5

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 106.8

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 169.2

PCR SuperMix for PAGE (+dye)

20-abx098004
  • Ask for price
  • Ask for price
  • Ask for price
  • 15 ml
  • 1 ml
  • 5 ml

PCR SuperMix for PAGE (+dye)

abx098004-100l 100 µl
EUR 237.5

PCR SuperMix for PAGE (+dye)

abx098004-200l 200 µl
EUR 337.5

Fast QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055002 200 rxn
EUR 426

Fast QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055004 400 rxn
EUR 682

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

OmniPCR Supermix w Fluorescent dye

MBA01-0100 100 rxns (2.5ml)
EUR 16

EnTurbo™ SYBR Green PCR SuperMix

EQ001 5mL
EUR 340

AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X)

MB204-P100 100 rxns2 x 1.25 mL
EUR 13

Green Two-Step qRT-PCR SuperMix

abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 777.6

Green One-Step qRT-PCR SuperMix

20-abx09803820ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix

20-abx09804220ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Green Two-Step qRT-PCR SuperMix

abx098035-100l 100 µl
EUR 925

Green Two-Step qRT-PCR SuperMix

abx098035-1ml 1 ml Ask for price

Green Two-Step qRT-PCR SuperMix

abx098035-200l 200 µl Ask for price

Green One-Step qRT-PCR SuperMix

abx098038-100l 100 µl
EUR 737.5

Green One-Step qRT-PCR SuperMix

abx098038-1ml 1 ml Ask for price

Green One-Step qRT-PCR SuperMix

abx098038-200l 200 µl
EUR 1050

Probe One-Step qRT-PCR SuperMix

abx098042-100l 100 µl
EUR 850

Probe One-Step qRT-PCR SuperMix

abx098042-1ml 1 ml Ask for price

Probe One-Step qRT-PCR SuperMix

abx098042-200l 200 µl
EUR 1400

Two-Step RT-PCR SuperMix (12 kb)

abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)

abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 910.8

Two-Step RT-PCR SuperMix (12 kb)

abx098025-100l 100 µl
EUR 887.5

Two-Step RT-PCR SuperMix (12 kb)

abx098025-1ml 1 ml Ask for price

Two-Step RT-PCR SuperMix (12 kb)

abx098025-200l 200 µl Ask for price

Two-Step RT-PCR SuperMix (15 kb)

abx098026-100l 100 µl
EUR 1087.5

Two-Step RT-PCR SuperMix (15 kb)

abx098026-1ml 1 ml Ask for price

Two-Step RT-PCR SuperMix (15 kb)

abx098026-200l 200 µl Ask for price

AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X)

MB202-P100 100 rxns2 x 1.25 mL
EUR 11

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

OmniPCR HS Supermix w Fluorescent dye

MBA03-0100 100 rxns2 x 1.25 mL
EUR 16

OmniPCR FAST Supermix w Fluorescent dye

MBA02-0100 100 rxns2 x 1.25 mL
EUR 16

OmniPCR Long Supermix w Fluorescent dye

MBA04-0100 100 rxns2 x 1.25 mL
EUR 16

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 1028.4

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-100l 100 µl
EUR 875

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-1ml 1 ml Ask for price

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-200l 200 µl Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-100l 100 µl
EUR 950

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-1ml 1 ml Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200l 200 µl Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-100l 100 µl
EUR 1212.5

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-1ml 1 ml Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-200l 200 µl Ask for price

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Fly First-Strand cDNA Synthesis SuperMix

abx098858-100l 100 µl
EUR 850

Fly First-Strand cDNA Synthesis SuperMix

abx098858-1ml 1 ml Ask for price

Fly First-Strand cDNA Synthesis SuperMix

abx098858-200l 200 µl
EUR 1037.5

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)
EUR 1062

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-100l 100 µl
EUR 1212.5

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-1ml 1 ml Ask for price

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-200l 200 µl Ask for price

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-20rxns20ulSystems 20 rxns × 20 ul Systems
EUR 878.4

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-100l 100 µl
EUR 950

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-1ml 1 ml Ask for price

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-200l 200 µl Ask for price

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007
  • Ask for price
  • Ask for price
  • 1 ml
  • 5 ml

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 961.2

Green One-Step qRT-PCR SuperMix (GC Rich)

20-abx09803920ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix (GC Rich)

20-abx09804120ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

abx098007-100l 100 µl
EUR 262.5

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

abx098007-200l 200 µl
EUR 475

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-100l 100 µl
EUR 1125

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-1ml 1 ml Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-200l 200 µl Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)

abx098039-100l 100 µl
EUR 450

Green One-Step qRT-PCR SuperMix (GC Rich)

abx098039-1ml 1 ml Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)

abx098039-200l 200 µl
EUR 1000

Probe One-Step qRT-PCR SuperMix (GC Rich)

abx098041-100l 100 µl
EUR 737.5

Probe One-Step qRT-PCR SuperMix (GC Rich)

abx098041-1ml 1 ml Ask for price

Probe One-Step qRT-PCR SuperMix (GC Rich)

abx098041-200l 200 µl
EUR 1050

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 693.6

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-100l 100 µl
EUR 850

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-1ml 1 ml Ask for price

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-200l 200 µl Ask for price

HyperScript First-Strand cDNA Synthesis SuperMix

K1073-100 100 rxn (20 uL/rxn)
EUR 282
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

HyperScript First-Strand cDNA Synthesis SuperMix

K1073-50 50 rxn (20 uL/rxn)
EUR 150
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

One-Step gDNA Removal and cDNA Synthesis SuperMix

20-abx09885920ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix

abx098859-100l 100 µl
EUR 1062.5

One-Step gDNA Removal and cDNA Synthesis SuperMix

abx098859-1ml 1 ml Ask for price

One-Step gDNA Removal and cDNA Synthesis SuperMix

abx098859-200l 200 µl
EUR 1425

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)

EQ014 5mL
EUR 340

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)

EQ013 5mL
EUR 340

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

abx098016-100l 100 µl
EUR 775

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

abx098016-1ml 1 ml Ask for price

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

abx098016-200l 200 µl
EUR 912.5

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

abx098021-100l 100 µl
EUR 962.5

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

abx098021-1ml 1 ml Ask for price

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

abx098021-200l 200 µl
EUR 1237.5

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)

20-abx09802220ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)

abx098022-100l 100 µl
EUR 1012.5

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)

abx098022-1ml 1 ml Ask for price

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)

abx098022-200l 200 µl
EUR 1325

One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)

20-abx09801520ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb)

20-abx09801720ulSystems
  • Ask for price
  • Ask for price
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)

abx098015-100l 100 µl
EUR 750

One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)

abx098015-1ml 1 ml
EUR 1737.5

One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)

abx098015-200l 200 µl
EUR 862.5

One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb)

abx098017-100l 100 µl
EUR 812.5
The 2 inside reference genes have been additional validated by analyzing the expression of four genes concerned in steroidal saponin biosynthesis pathways. In conclusion, ubiquitin-conjugating enzyme-E2-10 and elongation issue 1-alpha isoform can be utilized as essentially the most acceptable inside reference genes for qPCR evaluation in P. cyrtonema. This research additionally present a basis for future examine the molecular mechanism of steroidal saponin biosynthesis pathways in P. cyrtonema.

Leave a Reply

Your email address will not be published. Required fields are marked *