MicroarrayWorld Microarrays and gene expression profiling

Telomeres are a terminal “DNA cap” that stop chromosomal fusion and degradation. Nevertheless, growing older is inherent to life, and so is the lack of terminal sequences. Telomerase is a specialised reverse transcriptase encoded by self-splicing introns that counteract chromosome erosion. Telomerase exercise is noticed throughout early embryonic growth, however after the blastocyst stage, the expression of telomerase reduces.

The results of both inadequate or unrestrained telomerase exercise underscore the significance of ongoing research aimed toward elucidating the regulation of telomerase exercise in people. Within the current research, we aimed to standardize a simplified telomerase repeat-amplification protocol (TRAP) assay to detect telomerase exercise in unstimulated and PHA-stimulated mononuclear cells.

Choroid plexus (ChP), a vascularized secretory epithelium situated in all mind ventricles, performs vital roles in growth, homeostasis and mind restore. Reverse transcription quantitative real-time PCR (RT-qPCR) is a well-liked and helpful approach for measuring gene expression modifications and in addition broadly utilized in ChP research. Nevertheless, the reliability of RT-qPCR information is strongly depending on the selection of reference genes, that are purported to be steady throughout all samples.

On this research, we validated the expression of 12 properly established housekeeping genes in ChP in 2 unbiased experimental paradigms by utilizing common stability testing algorithms: BestKeeper, DeltaCq, geNorm and NormFinder. Rer1 and Rpl13a have been recognized as probably the most steady genes all through mouse ChP growth, whereas Hprt1 and Rpl27 have been probably the most steady genes throughout situations in a mouse sensory deprivation experiment.

As well as, Rpl13a, Rpl27 and Tbp have been mutually among the many prime 5 most steady genes in each experiments. Normalisation of Ttr and Otx2 expression ranges utilizing totally different housekeeping gene combos demonstrated the profound impact of reference gene selection on course gene expression. Our research emphasised the significance of validating and choosing steady housekeeping genes beneath particular experimental situations.

To attenuate pattern dilution impact on SARS-CoV-2 pool testing, we assessed analytical and diagnostic efficiency of a brand new methodology, specifically swab pooling. On this technique, swabs are pooled on the time of assortment, versus pooling of equal volumes from individually collected samples. Paired evaluation of pooled and particular person samples from 613 sufferers revealed 94 constructive people.

Having particular person testing as reference, no false-positives or false-negatives have been noticed for swab pooling. In further 18,922 sufferers screened with swab pooling (1,344 swimming pools), imply Cq variations between particular person and pool samples ranged from 0.1 (Cr.I. -0.98 to 1.17) to 2.09 (Cr.I. 1.24 to 2.94). Total, 19,535 asymptomatic sufferers have been screened utilizing 4,400 RT-qPCR assays. This corresponds to a rise of 4.Four occasions in laboratory capability and a discount of 77% in required assessments. Due to this fact, swab pooling represents a serious various for dependable and large-scale screening of SARS-CoV-2 in low prevalence populations.

Re-opening of communities within the midst of the continuing COVID-19 pandemic has ignited new waves of infections in lots of locations around the globe. Mitigating the danger of reopening would require widespread SARS-CoV-2 testing, which might be enormously facilitated by easy, fast, and cheap testing strategies. This research evaluates a number of protocols for RNA extraction and RT-qPCR which can be less complicated and cheaper than prevailing strategies. First, isopropanol precipitation is proven to supply an efficient technique of RNA extraction from nasopharyngeal (NP) swab samples. Second, direct addition of NP swab samples to RT-qPCRs is evaluated with out an RNA extraction step.

A easy, cheap swab assortment resolution appropriate for direct addition is validated utilizing contrived swab samples. Third, an open-source grasp combine for RT-qPCR is described that allows detection of viral RNA in NP swab samples with a restrict of detection of roughly 50 RNA copies per response. Quantification cycle (Cq) values for purified RNA from 30 recognized constructive medical samples confirmed a robust correlation (r2 = 0.98) between this home made grasp combine and business TaqPath grasp combine.

Lastly, end-point fluorescence imaging is discovered to supply an correct diagnostic readout with out requiring a qPCR thermocycler. Adoption of those easy, open-source strategies has the potential to cut back the time and expense of COVID-19 testing.

The warmth-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in uncooked milk, is a serious reason for destabilization and untimely spoilage of ultra-high temperature (UHT) milk and milk merchandise. To allow fast detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in uncooked milk, we developed two triplex qPCR assays taking into consideration species-dependent variations in AprX exercise. In addition to 5 species-specific hydrolysis probes, concentrating on the aprX gene, a common rpoB probe was included within the assay to find out the whole Pseudomonas counts.

For all six probes, linear regression traces between C_q worth and goal DNA focus have been obtained in singleplex in addition to in multiplex approaches, yielding R² values of > 0.975 and amplification efficiencies of 85-97%. Furthermore, excessive specificity was decided utilizing genomic DNA of 75 Pseudomonas strains, assigned to 57 species, and 40 different bacterial species as templates within the qPCR. Quantification of the goal species and whole Pseudomonas counts resulted in linear detection ranges of approx. 10³-10⁷ cfu/ml, which correspond properly to widespread Pseudomonas counts in uncooked milk.

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-02	Vazyme	500 rxns (20 μl/rxn)	EUR 96.44

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 434.8

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-02	Vazyme	500 rxns (20 μl/rxn)	EUR 112.5

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 507.2

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-02	Vazyme	500 rxns (20 μl/rxn)	EUR 112.5

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 507.2

Eva QPCR SuperMix Kit
K5052200	Biochain	200 reactions	EUR 188

Eva QPCR SuperMix Kit
K5052400	Biochain	400 reactions	EUR 316

Probe qPCR SuperMix
20-abx098040	Abbexa	EUR 1245.60 EUR 526.80 EUR 760.80	15 ml 1 ml 5 ml

Fast Eva QPCR SuperMix Kit
K5052002	Biochain	200 rxn	EUR 188

Power Eva QPCR SuperMix Kit
K5057200	Biochain	200 reactions	EUR 161

Power Eva QPCR SuperMix Kit
K5057400	Biochain	400 reactions	EUR 316

Green qPCR SuperMix
20-abx098031	Abbexa	EUR 1245.60 EUR 526.80 EUR 760.80	15 ml 1 ml 5 ml

Green qPCR SuperMix UDG
20-abx098032	Abbexa	EUR 1362.00 EUR 543.60 EUR 794.40	15 ml 1 ml 5 ml

Top Green qPCR SuperMix
20-abx098033	Abbexa	EUR 994.80 EUR 260.40 EUR 1362.00 EUR 493.20	15 ml 1 ml 25 ml 5 ml

Tip Green qPCR SuperMix
20-abx098034	Abbexa	EUR 1161.60 EUR 276.00 EUR 1596.00 EUR 560.40	15 ml 1 ml 25 ml 5 ml

Library Quantification qPCR SuperMix
20-abx098896	Abbexa	EUR 276.00 EUR 493.20	1 ml 5 ml

Top Green qPCR SuperMix (+Dye II)
20-abx098890	Abbexa	EUR 994.80 EUR 260.40 EUR 1362.00 EUR 493.20	15 ml 1 ml 25 ml 5 ml

Tip Green qPCR SuperMix (+Dye II)
20-abx098891	Abbexa	EUR 1161.60 EUR 276.00 EUR 1596.00 EUR 560.40	15 ml 1 ml 25 ml 5 ml

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	EUR 828.00 EUR 678.00	100 rxns ×20 ul Systems 50 rxns × 20 ul Systems

HyperScript RT SuperMix for qPCR
K1074-100	ApexBio	100 rxn (20 uL/rxn)	EUR 350
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR
K1074-50	ApexBio	50 rxn (20 uL/rxn)	EUR 188
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HiScript II Q RT SuperMix for qPCR
R222-01	Vazyme	100 rxn (20 μl/rxn)	EUR 282

VAHTS Library Quantification Kit for Illumina (Low ROX Premixed)
NQ103	Vazyme	500 rxn	EUR 349.2

VAHTS Library Quantification Kit for Illumina (High ROX Premixed)
NQ104	Vazyme	500 rxn	EUR 349.2

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 861.6

HiScript II Q Select RT SuperMix for qPCR
R232-01	Vazyme	100 rxn (20 μl/rxn)	EUR 282

HiScript III RT SuperMix for qPCR (+g DNA wiper)
R323-01	Vazyme	100 rxn (20 μl/rxn)	EUR 351.6

HyperScript RT SuperMix for qPCR (with gDNA wiper)
K1174-100	ApexBio	100 rxns	EUR 380
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR (with gDNA wiper)
K1174-50	ApexBio	50 rxns	EUR 220
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)
R233-01	Vazyme	100 rxn (20 μl/rxn)	EUR 292.8

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01	Vazyme	100 rxns (21 μl/rxn)	EUR 174

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055004	Biochain	400 rxn	EUR 609

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055200	Biochain	200 reactions	EUR 381

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055400	Biochain	400 reactions	EUR 609

Fast QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055002	Biochain	200 rxn	EUR 380

PCR SuperMix
20-abx098887	Abbexa	EUR 326.40 EUR 693.60	1 ml 5 ml

Animal Detection Probe qPCR Super PreMix
QV114-EN01	Vazyme	400 rxns	EUR 128

Animal Detection Probe qPCR Super PreMix
QV114-EN02	Vazyme	800 rxns	EUR 230.4

PCR SuperMix (+dye)
20-abx098003	Abbexa	EUR 493.20 EUR 226.80 EUR 7408.80 EUR 309.60	15 ml 1 ml 480 ml 5 ml

PCR SuperMix (-dye)
20-abx098002	Abbexa	EUR 493.20 EUR 226.80 EUR 309.60	15 ml 1 ml 5 ml

Plus PCR SuperMix
20-abx098888	Abbexa	EUR 292.80 EUR 560.40	1 ml 5 ml

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

T PCR SuperMix (-dye)
20-abx098005	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

T PCR SuperMix (+dye)
20-abx098006	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 678.00 EUR 577.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

EasyPfu PCR SuperMix (-dye)
20-abx098009	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

FastPfu PCR SuperMix (-dye)
20-abx098010	Abbexa	EUR 292.80 EUR 594.00	1 ml 5 ml

Library Amplification SuperMix
20-abx098894	Abbexa	EUR 309.60 EUR 644.40	1 ml 5 ml

QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054004	Biochain	400 rxn	EUR 635

QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054200	Biochain	200 reactions	EUR 418

QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054400	Biochain	400 reactions	EUR 635

PCR SuperMix for PAGE (+dye)
20-abx098004	Abbexa	EUR 493.20 EUR 226.80 EUR 309.60	15 ml 1 ml 5 ml

OneScriptcDNA Synthesis SuperMix
G451	ABM	25 x 20 ul reactions	EUR 106.8

OneScriptcDNA Synthesis SuperMix
G452	ABM	100 x 20 ul reactions	EUR 169.2

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054002	Biochain	200 rxn	EUR 418

Probe One-Step qRT-PCR SuperMix
20-abx09804220ulSystems	Abbexa	EUR 710.40 EUR 1228.80	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 202.8

Green Two-Step qRT-PCR SuperMix
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 777.6

Green One-Step qRT-PCR SuperMix
20-abx09803820ulSystems	Abbexa	EUR 594.00 EUR 895.20	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Two-Step RT-PCR SuperMix (12 kb)
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 910.8

All-in-One cDNA Synthesis SuperMix
B24403	Bimake	200 ractions	EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408	Bimake	1000 ractions	EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 1028.4

Probe One-Step qRT-PCR SuperMix (GC Rich)
20-abx09804120ulSystems	Abbexa	EUR 594.00 EUR 895.20	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Fly First-Strand cDNA Synthesis SuperMix
20-abx09885820ulSystems	Abbexa	EUR 861.60 EUR 693.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems	Abbexa	20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)	EUR 1062

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-20rxns20ulSystems	Abbexa	20 rxns × 20 ul Systems	EUR 878.4

ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
45900-5mL	AAT Bioquest	5 mL	EUR 222
Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
45901-50mL	AAT Bioquest	50 mL	EUR 446
Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
20-abx098007	Abbexa	EUR 260.40 EUR 427.20	1 ml 5 ml

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 961.2

Green One-Step qRT-PCR SuperMix (GC Rich)
20-abx09803920ulSystems	Abbexa	EUR 710.40 EUR 1228.80	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 693.6

HyperScript First-Strand cDNA Synthesis SuperMix
K1073-100	ApexBio	100 rxn (20 uL/rxn)	EUR 352
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

HyperScript First-Strand cDNA Synthesis SuperMix
K1073-50	ApexBio	50 rxn (20 uL/rxn)	EUR 188
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
45900	AAT Bioquest	5 mL	EUR 222

ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
45901	AAT Bioquest	50 mL	EUR 446

One-Step gDNA Removal and cDNA Synthesis SuperMix
20-abx09885920ulSystems	Abbexa	EUR 1228.80 EUR 895.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	EUR 744.00 EUR 627.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems	Abbexa	EUR 1045.20 EUR 794.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
20-abx09802220ulSystems	Abbexa	EUR 1128.00 EUR 828.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)
20-abx09801520ulSystems	Abbexa	EUR 693.60 EUR 594.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb)
20-abx09801720ulSystems	Abbexa	EUR 794.40 EUR 644.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

All-in-One First-Strand cDNA Synthesis SuperMix for PCR
abx098023-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 878.4

HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper)
K1173-100	ApexBio	100 rxns	EUR 380
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper)
K1173-50	ApexBio	50 rxns	EUR 220
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)
20-abx098008	Abbexa	EUR 260.40 EUR 427.20	1 ml 5 ml

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)
Q621-EN01	Vazyme	200 rxns (20 μl/rxn)	EUR 208.69

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)
Q621-EN02	Vazyme	1,000 rxns (20 μl/rxn)	EUR 811.58

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)
Q621-EN03	Vazyme	10,000 rxns (20 μl/rxn)	EUR 6956.4

HotTaq Probe qPCR Mix (ROX)
BT11001	Bioatlas	250rxn	EUR 130.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)
BT11101	Bioatlas	250rxn	EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (no ROX)
BT11002	Bioatlas	250rxn	EUR 130.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Genorise® 2 x qPCR HotStart Super Mix
GR108001	Genorise Scientific	2.0 ml	EUR 199

HotTaq EvaGreen qPCR Mix (no ROX)
BT11102	Bioatlas	250rxn	EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

TaqProbe 5X qPCR MasterMix-Low ROX
MasterMix-5PL	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

TaqProbe 2X qPCR MasterMix-Low ROX
MasterMix-PL	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 168

ROX Dye qPCR Calibration Solution *10,000X*
67034-100ul	AAT Bioquest	100 ul	EUR 301
Description: The AAT Bioquest ROX dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudioâ„¢, and ViiAâ„¢ 7 systems.

BrightGreen 5X qPCR MasterMix-Low ROX
MasterMix-5L	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 451.2

ROX reference dye for qPCR, 1 mL
41110	Lumiprobe	1 mL	EUR 62.4

Utility of the assay utilizing 60 uncooked milk samples from totally different dairies confirmed good settlement of whole Pseudomonas counts calculated by qPCR with cell counts derived from cultivation. Moreover, a remarkably excessive variability relating to the species composition was noticed for every milk pattern, whereby P. lundensis and P. proteolytica/P. gessardii have been the predominant species detected.