Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

An correct genetic diagnostic is essential for enough affected person administration and the suitability of healthcare techniques. The scientific problem lies in growing strategies to discriminate these sufferers with sure genetic variations current in tumor cells at low concentrations. We report a technique referred to as enhanced uneven blocked qPCR (EAB-qPCR) that promotes the blocker annealing towards the primer-template hybrid controlling thermal biking and response circumstances with nonmodified oligonucleotides. Actual-time fluorescent amplification curves of wild-type alleles had been delayed by about eight cycles for EAB-qPCR, in comparison with standard blocked qPCR approaches.

This methodology diminished the amplification of native DNA variants (blocking proportion 99.7%) and enabled the efficient enrichment of low-level DNA mutations. Wonderful efficiency was estimated for the detection of mutated alleles in sensitivity (as much as 0.5% mutant/whole DNA) and reproducibility phrases, with a relative commonplace deviation under 2.8%. The strategy was efficiently utilized to the mutational evaluation of metastatic colorectal carcinoma from biopsied tissues.

The decided single-nucleotide mutations within the KRAS oncogene (codon 12-13) completely agreed with these obtained from next-generation sequencing. EAB-qPCR is an correct low cost methodology and might be simply included into day by day routine to detect mutant alleles. Therefore, these options are particularly attention-grabbing to facilitate the analysis and prognosis of a number of scientific ailments.

Quantification of Actaea racemosa L. (black cohosh) from a few of its potential adulterants utilizing qPCR and dPCR strategies

The demand for well-liked pure well being merchandise (NHPs) similar to Black Cohosh is growing significantly, which in flip challenges high quality assurance (QA) all through the availability chain. To detect and quantify the goal species current in a given NHP, DNA-based molecular strategies similar to Actual-time quantitative PCR (qPCR) and digital PCR (dPCR) are commonplace instruments within the meals and pathogen testing industries.
There’s a hole within the literature regarding validated quantitative PCR strategies for botanicals that may be utilized for QA and good manufacturing practices. The target of this research is to develop an environment friendly quantification methodology utilizing qPCR and dPCR strategies for the detection and quantification of Actaea racemosa (Black cohosh) NHPs from its potential adulterants. These developed strategies are validated for applicability on business NHPs.
Species-specific hydrolysis probe assays had been designed to research the black cohosh NHPs utilizing qPCR and dPCR strategies. The outcomes confirmed that the developed qPCR and dPCR strategies are extremely exact for figuring out and quantifying black cohosh NHPs, indicating their potential applicability in future routine industrial and laboratory testing. This permits a single qPCR check to find out not solely the presence of a particular botanical, but additionally the quantity when combined with an adulterant.

Quantification of Legionella pneumophila by qPCR and tradition in faucet water with completely different concentrations of residual disinfectants and heterotrophic micro organism

Legionellosis prevalence is growing in the USA. This illness is precipitated primarily by the bacterium Legionella pneumophila present in water and transmitted by aerosol inhalation. This pathogen has a gradual progress fee and may “disguise” in amoeba, making it tough to watch by the normal tradition methodology on selective media. Faucet water samples (n = 358) collected throughout the USA had been examined for L. pneumophila by each tradition and quantitative Polymerase Chain Response (qPCR).
The presence of different micro organism was quantified by heterotrophic plate counts (HPC). Residual disinfectant concentrations (free chlorine or monochloramine) had been measured in all samples. Legionella pneumophila had the best prevalence and focus within the chlorinated water samples that had a free‑chlorine worth of lower than 0.2 mg Cl2/L. In whole, 24% (87/358) of the samples had been constructive for L. pneumophila both by qPCR or 3% (11/358) had been constructive by tradition.
Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene
In chloramine-treated samples, L. pneumophila was detected by qPCR in 21% (31/148) and 1% (2/148) by tradition, regardless of a excessive monochloramine residual >1 mg Cl2/L. Regardless of the presence of a excessive disinfectant residual (>1 mg Cl2/L), HPC counts had been substantial. This research signifies that each tradition and qPCR strategies have limitations when predicting a possible danger for illness related to L. pneumophila in faucet water. Measuring disinfectant residuals and quantifying HPC in water samples could also be helpful adjunct parameters for lowering Legionellosis’ danger from public water provides at high-risk areas.

PANDAA deliberately violates standard qPCR design to allow sturdy, mismatch-agnostic detection of extremely polymorphic pathogens

Delicate and reproducible diagnostics are elementary to containing the unfold of present and rising pathogens. Regardless of the reliance of scientific virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding areas produces false-negative outcomes.
We systematically violated canonical qPCR design rules to develop a Pan-Degenerate Amplification and Adaptation (PANDAA), a degree mutation assay that mitigates the influence of sequence variation on probe-based qPCR efficiency. Utilizing HIV-1 as a mannequin system, we optimized and validated PANDAA to detect HIV drug resistance mutations (DRMs). Extremely-degenerate primers with 3′ termini overlapping the probe-binding web site adapt the goal by site-directed mutagenesis throughout qPCR to exchange DRM-proximal sequence variation.

2x SYBR Green qPCR Master Mix (High ROX)
B21403 25 ml
EUR 856
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)
B21702 5 ml
EUR 224
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)
B21703 25 ml
EUR 856
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix
B21202 5 mL
EUR 224
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2x SYBR Green qPCR Master Mix
B21203 25 mL
EUR 856
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

SYBR Green qPCR Master Mix (High ROX)
HY-K0521 1 mL (100 rxns)
EUR 113

SYBR Green qPCR Master Mix (Low ROX)
HY-K0522 5 mL (500 rxns )
EUR 257

SYBR Green qPCR Master Mix (No ROX)
HY-K0523 5 mL (500 rxns )
EUR 257

SYBR Green qPCR Master Mix
HY-K0501 5 mL (500 rxns)
EUR 263

ig SYBR Green qPCR 2X Master Mix - 500 Reactions
3356 1/EA
EUR 288

AceQ qPCR SYBR® Green Master Mix
Q111-02 500 rxn (20 μl/rxn)
EUR 221

AceQ qPCR SYBR® Green Master Mix
Q111-03 2500 rxn (20 μl/rxn)
EUR 646

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Q511-02 500 rxn (20 μl/rxn)
EUR 221

AceQ Universal SYBR qPCR Master Mix
Q511-03 2500 rxn (20 μl/rxn)
EUR 646

ChamQ Universal SYBR qPCR Master Mix
Q711-02 500 rxn (20 μl/rxn)
EUR 221

ChamQ Universal SYBR qPCR Master Mix
Q711-03 2500 rxn (20 μl/rxn)
EUR 646

miRNA Universal SYBR® qPCR Master Mix
MQ101-01 125 rxn(20 μl/rxn)
EUR 138

miRNA Universal SYBR® qPCR Master Mix
MQ101-02 500 rxn(20 μl/rxn)
EUR 242

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-001 1ml
EUR 280

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-002 2x1ml
EUR 482

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P0346-005 5x1ml
EUR 828

amfiSure Ultra Fidelity PCR Master Mix(2X)
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HotStart PCR Master mix (2X, Green Dye)
BS9291 5X1mL, 5ml
EUR 209.5
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

HotStart PCR Master mix (2X, Green Dye)
BS9292 1ml
EUR 96.4
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)
31014 2x1mL
EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)
31014-1 5x1mL
EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)
31014-2 50x1mL
EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)
31015-1 5x1mL
EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)
31015-2 50x1mL
EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)
31042-1 5x1mL
EUR 272
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)
31042-20mL 2x10mL
EUR 494
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)
31042-T 1mL
EUR 114
Description: Minimum order quantity: 1 unit of 1mL

Bio-Star Mastermix with SYBR (2X) High-ROX
Y240 100 rcs (2,5 ml)
EUR 102

Bio-Star Mastermix with SYBR (2X) Low-ROX
Y250 100 rcs (2,5 ml)
EUR 102

Taqman Master Mix-ROX
M1124-500
EUR 441

SYBR Green I
B1747-25
EUR 1164

SYBR Green I
B1747-5
EUR 387

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)
31014-T 1mL
EUR 134
Description: Minimum order quantity: 1 unit of 1mL

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)
31015 2x1mL
EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)
31015-T 1mL
EUR 134
Description: Minimum order quantity: 1 unit of 1mL

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-001 1ml
EUR 146

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-002 2x1ml
EUR 207

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-005 5x1ml
EUR 269

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-010 10x1ml
EUR 448

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-050 50x1ml
EUR 1738

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-001 1ml
EUR 146

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-002 2x1ml
EUR 207

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-005 5x1ml
EUR 266

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-010 10x1ml
EUR 442

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-050 50x1ml
EUR 1670

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-001 1ml
EUR 146

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-002 2x1ml
EUR 207

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-005 5x1ml
EUR 266

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-010 10x1ml
EUR 442

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-050 50x1ml
EUR 1670

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-001 1ml
EUR 174

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-002 2x1ml
EUR 266

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-005 5x1ml
EUR 469

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-010 10x1ml
EUR 739

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-050 50x1ml
EUR 2885

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-001 1ml
EUR 174

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-002 2x1ml
EUR 266

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-005 5x1ml
EUR 469

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-010 10x1ml
EUR 739

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-050 50x1ml
EUR 334

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-001 1ml
EUR 174

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-002 2x1ml
EUR 266

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-003 5x1ml
EUR 469

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-004 10x1ml
EUR 739

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-005 50x1ml
EUR 2885

KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-ROX
MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

HotTaq Probe qPCR Mix (ROX)
BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)
BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Fast Probe Master Mix with Rox (200 rxn)
31016 2x1mL
EUR 234
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Probe Master Mix with Rox (500 rxn)
31016-1 5x1mL
EUR 466
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Probe Master Mix with Rox (5000 rxn)
31016-2 50x1mL
EUR 3871
Description: Minimum order quantity: 1 unit of 50x1mL

RT Master Mix for qPCR
HY-K0510 1 mL (100 rxns)
EUR 291

AceQ qPCR Probe Master Mix
Q112-02 500 rxn (20 μl/rxn)
EUR 221

AceQ qPCR Probe Master Mix
Q112-03 2500 rxn (20 μl/rxn)
EUR 646

Taqman Master Mix-Low ROX
M1123-500
EUR 441

EZSolution? SYBR Green I
B1748-1
EUR 620

EZSolution? SYBR Green I
B1748-500
EUR 430

BrightGreen Express 2X qPCR MasterMix-ROX
MasterMix-ER 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

TaqProbe 2X qPCR MasterMix-Low ROX
MasterMix-PL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)
9-31045
  • EUR 114.00
  • EUR 482.00
  • EUR 266.00
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)
9-31046
  • EUR 114.00
  • EUR 482.00
  • EUR 266.00
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

HotTaq Probe qPCR Mix (no ROX)
BT11002 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)
BT11102 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Pfu 2x Master Mix - 200 Reactions
3326 1/EA
EUR 300

amfiSure ONE PCR Master Mix(2X)
P7000-005 5x1 ml
EUR 219

amfiSure ONE PCR Master Mix(2X)
P7000-010 10x1 ml
EUR 317

amfiSure ONE PCR Master Mix(2X)
P7000-050 50x1 ml
EUR 1211

amfiSure ONE PCR Master Mix(2X)
P7000-100 100x1 ml
EUR 2102

SensiFAST SYBR Lo-ROX Mix, 50 mL
BIO-94050 5000 rxn Ask for price

SensiFAST SYBR No-ROX Mix, 50 mL
BIO-98050 5000 rxn Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

BrightGreen Express 2X qPCR MasterMix-Low ROX
MasterMix-EL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

Fast Probe Master Mix with Rox, trial size (100 rxn)
31016-T 1mL
EUR 141
Description: Minimum order quantity: 1 unit of 1mL

Accuris qMax Probe No Rox qPCR Mix
PR2001-N-1000 1 PC
EUR 467.74
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

SensiFAST Lyo-Ready No-ROX Mix, 2x
BIO-11060 10 x 10ml Ask for price

SensiFAST Lyo-Ready No-ROX Mix, 2x
BIO-11061 5ml Ask for price

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-010 2x50 rxns
EUR 253

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-025 5X50 rxns
EUR 455

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-050 10X50 rxns
EUR 779

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-100 20x50 rxns
EUR 1333

Taq PCR Master Mix (2X, Blue Dye)
BS9295 1ml
EUR 84.8
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Taq PCR Master Mix (2X, Blue Dye)
BS9296 5ml
EUR 189.2
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Taq PCR Master Mix (2X, Red Dye)
BS9297 1ml
EUR 84.8
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Taq PCR Master Mix (2X, Red Dye)
BS9298 5ml
EUR 189.2
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions 100 Reactions
EUR 327

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (500 reactions)
31044-1 1kit
EUR 330
Description: Minimum order quantity: 1 unit of 1kit

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (100 reactions)
31044-T 1kit
EUR 114
Description: Minimum order quantity: 1 unit of 1kit

2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn
W0655-1 NULL
EUR 0

2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn
W0655-5 NULL
EUR 0

Bio-Star Mastermix with SYBR (2X) BLUE
Y220 100 rcs (2,5 ml)
EUR 94

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500
HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500
HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn)
QPCR001-R 4X1.25ml
EUR 252.71
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials
S210 100 rcs (2,5 ml)
EUR 138

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials
S210L 500 rcs (12,5 ml)
EUR 523

Fast EvaGreen Master Mix for qPCR(200 rxn)
31003 2x1ML
EUR 204
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)
31003-1 5x1ML
EUR 386
Description: Minimum order quantity: 1 unit of 5x1ML

Fast EvaGreen Master Mix for qPCR (5000 rxn)
31003-2 50x1ML
EUR 3116
Description: Minimum order quantity: 1 unit of 50x1ML

Fast Plus EvaGreen qPCR Master Mix (200 rxn)
31020 2x1mL
EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix (500 rxn)
31020-1 5x1mL
EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)
31020-2 50x1mL
EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

RT Master Mix for qPCR (gDNA digester plus)
HY-K0511 1 mL (100 rxns)
EUR 315

Hot Start Taq 2x Master Mix - 500 Reactions
3296 1/EA
EUR 268

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-001 1ml
EUR 146

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-002 2x1ml
EUR 207

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-005 5x1ml
EUR 266

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-010 10x1ml
EUR 442

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-050 50x1ml
EUR 1670

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-001 1ml
EUR 174

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-002 2x1ml
EUR 266

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-005 5x1ml
EUR 469

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-010 10x1ml
EUR 739

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-050 50x1ml
EUR 2885

2xEs Taq Master Mix (with Dyes)
W0690-1 NULL
EUR 0

2xEs Taq Master Mix (with Dyes)
W0690-5 NULL
EUR 0

SYBR Green I Nucleic Acid Gel Stain
HY-K1004 100 μL
EUR 113

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
S170 100 rcs (2,5 ml)
EUR 138

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
S170L 500 rcs (12,5 ml)
EUR 523

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S175 100 rcs (2,5 ml)
EUR 138

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S175L 500 rcs (12,5 ml)
EUR 523

qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S215 100 rcs (2,5 ml)
EUR 159

qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S215L 500 rcs (12,5 ml)
EUR 523

qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S220 100 rcs (2,5 ml)
EUR 159

qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S220L 500 rcs (12,5 ml)
EUR 612

qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials
S225 100 rcs (2,5 ml)
EUR 159

qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials
S225L 500 rcs (12,5 ml)
EUR 612

Bio-Star Mastermix with SYBR (2X) no dye
Y210 100 rcs (2,5 ml)
EUR 89

Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn)
QPCR002-L 4X1.25ml
EUR 252.71
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn
AZ-2301 200 Rxn
EUR 179

AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn
AZ-2305 500 Rxn
EUR 320

AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn
AZ-2320 2000 Rxn
EUR 996

AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn
AZ-2325 5000 Rxn
EUR 2348

AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn
AZ-2401 200 Rxn
EUR 179

AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn
AZ-2405 500 Rxn
EUR 320

AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn
AZ-2420 2000 Rxn
EUR 996

AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn
AZ-2421 5000 Rxn
EUR 2348

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions)
31041-1 5x1mL
EUR 272
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions)
31041-20mL 2x10mL
EUR 494
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions)
31041-T 1mL
EUR 114
Description: Minimum order quantity: 1 unit of 1mL

Deoxy+ SYBR Green OneStep qRT-PCRKit wi ROX, 100 preps
FYT504-100P 1 vial Ask for price

Deoxy+ SYBR Green OneStep qRT-PCRwi ROX Kit, 50 preps
FYT504-50P 1 vial Ask for price

Green qPCR SuperMix
20-abx098031
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

qPCR Master Mix E4 (SafeLine: EvaGreen, dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S180 100 rcs (2,5 ml)
EUR 159

qPCR Master Mix E4 (SafeLine: EvaGreen, dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S180L 500 rcs (12,5 ml)
EUR 612

qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials
S185 100 rcs (2,5 ml)
EUR 159

qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials
S185L 500 rcs (12,5 ml)
EUR 612

2×Taq PCR Master Mix(with dye)
K1034-1 1 ml
EUR 73

2×Taq PCR Master Mix(with dye)
K1034-100 100×1 ml
EUR 792

2×Taq PCR Master Mix(with dye)
K1034-20 20×1 ml
EUR 282

2×Taq PCR Master Mix(with dye)
K1034-5 5×1 ml
EUR 119

2×Taq PCR Master Mix(with dye)
K1034-50 50×1 ml
EUR 514

Jade? Mix-ROX
M1108-500
EUR 441

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)
105-520 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)
105-522 10 x 1,25 ml
EUR 1719

One.Step RT-qPCR with EvaGreen / HighROX (2X concentrated)
105-530 2 x 1,25 ml
EUR 427

One.Step RT-qPCR with EvaGreen / HighROX (2X concentrated)
105-532 10 x 1,25 ml
EUR 1652

ACCUZYME Mix, 2x
BIO-25028 500 Reactions Ask for price

MyTaq Mix, 2x
BIO-25041 200 Reactions Ask for price

MyTaq Mix, 2x
BIO-25041/S Sample Ask for price

MyTaq Mix, 2x
BIO-25042 1000 Reactions Ask for price

MyFi Mix, 2x
BIO-25049 100 Reactions Ask for price

MyFi Mix, 2x
BIO-25049/S Sample Ask for price

MyFi Mix, 2x
BIO-25050 500 Reactions Ask for price

Ranger Mix, 2x
BIO-25051/S Sample Ask for price

Ranger Mix, 2x
BIO-25052 500 Reactions Ask for price

Jade? Master Mix
M1105-500
EUR 441

Taqman Master Mix
M1121-500
EUR 441

Fast EvaGreen Master Mix for qPCR, trial size (100 rxn)
31003-T 1ML
EUR 123
Description: Minimum order quantity: 1 unit of 1ML

Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn)
31020-T 1mL
EUR 134
Description: Minimum order quantity: 1 unit of 1mL
PANDAA-quantified DRMs current at frequency ≥5% (2 h from nucleic acid to consequence) with a sensitivity and specificity of 96.9% and 97.5%, respectively. PANDAA is an revolutionary development with applicability to any pathogen the place target-proximal genetic variability hinders diagnostic growth.
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