Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

An correct genetic diagnostic is essential for enough affected person administration and the suitability of healthcare techniques. The scientific problem lies in growing strategies to discriminate these sufferers with sure genetic variations current in tumor cells at low concentrations. We report a technique referred to as enhanced uneven blocked qPCR (EAB-qPCR) that promotes the blocker annealing towards the primer-template hybrid controlling thermal biking and response circumstances with nonmodified oligonucleotides. Actual-time fluorescent amplification curves of wild-type alleles had been delayed by about eight cycles for EAB-qPCR, in comparison with standard blocked qPCR approaches.

This methodology diminished the amplification of native DNA variants (blocking proportion 99.7%) and enabled the efficient enrichment of low-level DNA mutations. Wonderful efficiency was estimated for the detection of mutated alleles in sensitivity (as much as 0.5% mutant/whole DNA) and reproducibility phrases, with a relative commonplace deviation under 2.8%. The strategy was efficiently utilized to the mutational evaluation of metastatic colorectal carcinoma from biopsied tissues.

The decided single-nucleotide mutations within the KRAS oncogene (codon 12-13) completely agreed with these obtained from next-generation sequencing. EAB-qPCR is an correct low cost methodology and might be simply included into day by day routine to detect mutant alleles. Therefore, these options are particularly attention-grabbing to facilitate the analysis and prognosis of a number of scientific ailments.

Quantification of Actaea racemosa L. (black cohosh) from a few of its potential adulterants utilizing qPCR and dPCR strategies

The demand for well-liked pure well being merchandise (NHPs) similar to Black Cohosh is growing significantly, which in flip challenges high quality assurance (QA) all through the availability chain. To detect and quantify the goal species current in a given NHP, DNA-based molecular strategies similar to Actual-time quantitative PCR (qPCR) and digital PCR (dPCR) are commonplace instruments within the meals and pathogen testing industries.
There’s a hole within the literature regarding validated quantitative PCR strategies for botanicals that may be utilized for QA and good manufacturing practices. The target of this research is to develop an environment friendly quantification methodology utilizing qPCR and dPCR strategies for the detection and quantification of Actaea racemosa (Black cohosh) NHPs from its potential adulterants. These developed strategies are validated for applicability on business NHPs.
Species-specific hydrolysis probe assays had been designed to research the black cohosh NHPs utilizing qPCR and dPCR strategies. The outcomes confirmed that the developed qPCR and dPCR strategies are extremely exact for figuring out and quantifying black cohosh NHPs, indicating their potential applicability in future routine industrial and laboratory testing. This permits a single qPCR check to find out not solely the presence of a particular botanical, but additionally the quantity when combined with an adulterant.

Quantification of Legionella pneumophila by qPCR and tradition in faucet water with completely different concentrations of residual disinfectants and heterotrophic micro organism

Legionellosis prevalence is growing in the USA. This illness is precipitated primarily by the bacterium Legionella pneumophila present in water and transmitted by aerosol inhalation. This pathogen has a gradual progress fee and may “disguise” in amoeba, making it tough to watch by the normal tradition methodology on selective media. Faucet water samples (n = 358) collected throughout the USA had been examined for L. pneumophila by each tradition and quantitative Polymerase Chain Response (qPCR).
The presence of different micro organism was quantified by heterotrophic plate counts (HPC). Residual disinfectant concentrations (free chlorine or monochloramine) had been measured in all samples. Legionella pneumophila had the best prevalence and focus within the chlorinated water samples that had a free‑chlorine worth of lower than 0.2 mg Cl2/L. In whole, 24% (87/358) of the samples had been constructive for L. pneumophila both by qPCR or 3% (11/358) had been constructive by tradition.
Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene
In chloramine-treated samples, L. pneumophila was detected by qPCR in 21% (31/148) and 1% (2/148) by tradition, regardless of a excessive monochloramine residual >1 mg Cl2/L. Regardless of the presence of a excessive disinfectant residual (>1 mg Cl2/L), HPC counts had been substantial. This research signifies that each tradition and qPCR strategies have limitations when predicting a possible danger for illness related to L. pneumophila in faucet water. Measuring disinfectant residuals and quantifying HPC in water samples could also be helpful adjunct parameters for lowering Legionellosis’ danger from public water provides at high-risk areas.

PANDAA deliberately violates standard qPCR design to allow sturdy, mismatch-agnostic detection of extremely polymorphic pathogens

Delicate and reproducible diagnostics are elementary to containing the unfold of present and rising pathogens. Regardless of the reliance of scientific virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding areas produces false-negative outcomes.
We systematically violated canonical qPCR design rules to develop a Pan-Degenerate Amplification and Adaptation (PANDAA), a degree mutation assay that mitigates the influence of sequence variation on probe-based qPCR efficiency. Utilizing HIV-1 as a mannequin system, we optimized and validated PANDAA to detect HIV drug resistance mutations (DRMs). Extremely-degenerate primers with 3′ termini overlapping the probe-binding web site adapt the goal by site-directed mutagenesis throughout qPCR to exchange DRM-proximal sequence variation.

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B21203 25 mL
EUR 1027.2
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EUR 135.6

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EUR 99

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17277 5 mL
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BS9292 1ml
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Y240 100 rcs (2,5 ml)
EUR 122.4

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Y250 100 rcs (2,5 ml)
EUR 122.4

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RK21206 1 ml Ask for price

Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)
31014 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)
31014-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

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31014-2 50x1mL
EUR 4291.2
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)
31015-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)
31015-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)
31042-1 5x1mL
EUR 326.4
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)
31042-20mL 2x10mL
EUR 592.8
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)
31042-T 1mL
EUR 136.8
Description: Minimum order quantity: 1 unit of 1mL

Taqman Master Mix-ROX
M1124-500
EUR 529.2

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-001 1ml
EUR 175.2

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-002 2x1ml
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amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-005 5x1ml
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amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-010 10x1ml
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amfiSure qGreen Q-PCR Master Mix(2X), High Rox
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Q5602-050 50x1ml
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Q5603-005 5x1ml
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Q5603-010 10x1ml
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Q5603-050 50x1ml
EUR 2004

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Q5700-005 5x1ml
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amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-005 5x1ml
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amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-010 10x1ml
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Q5702-050 50x1ml
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Q5703-001 1ml
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Q5703-002 2x1ml
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Q5703-003 5x1ml
EUR 562.8

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Q5703-004 10x1ml
EUR 886.8

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Q5703-005 50x1ml
EUR 3462

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B1747-25
EUR 1396.8

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B1747-5
EUR 464.4

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)
31014-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)
31015 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)
31015-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

Fast Probe Master Mix with Rox (200 rxn)
31016 2x1mL
EUR 280.8
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Probe Master Mix with Rox (500 rxn)
31016-1 5x1mL
EUR 559.2
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Probe Master Mix with Rox (5000 rxn)
31016-2 50x1mL
EUR 4645.2
Description: Minimum order quantity: 1 unit of 50x1mL

KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

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MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

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MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 451.2

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173L 960 reactions x 20 µl
EUR 789.36
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173S 192 reactions x 20 µl
EUR 197.34
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

Taqman Master Mix-Low ROX
M1123-500
EUR 529.2

EZSolution? SYBR Green I
B1748-1
EUR 744

EZSolution? SYBR Green I
B1748-500
EUR 516

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*
17282 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*
17283 5 mL
EUR 308

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17284 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*
17285 5 mL
EUR 308

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17286 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*
17287 5 mL
EUR 308

HotTaq Probe qPCR Mix (ROX)
BT11001 250rxn
EUR 130.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)
BT11101 250rxn
EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RT Master Mix for qPCR
HY-K0510 1 mL (100 rxns)
EUR 349.2

AceQ qPCR Probe Master Mix
Q112-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR Probe Master Mix
Q112-03 2500 rxn (20 μl/rxn)
EUR 775.2

BrightGreen Express 2X qPCR MasterMix-ROX
MasterMix-ER 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 451.2

TaqProbe 2X qPCR MasterMix-Low ROX
MasterMix-PL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

Pfu 2x Master Mix - 200 Reactions
3326 1/EA
EUR 360

amfiSure ONE PCR Master Mix(2X)
P7000-005 5x1 ml
EUR 262.8

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P7000-010 10x1 ml
EUR 380.4

amfiSure ONE PCR Master Mix(2X)
P7000-050 50x1 ml
EUR 1453.2

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P7000-100 100x1 ml
EUR 2522.4

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RK20717 1mL Ask for price

SensiFAST SYBR Lo-ROX Mix, 50 mL
BIO-94050 5000 rxn Ask for price

SensiFAST SYBR No-ROX Mix, 50 mL
BIO-98050 5000 rxn Ask for price

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)
9-31045
  • EUR 136.80
  • EUR 578.40
  • EUR 319.20
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)
9-31046
  • EUR 136.80
  • EUR 578.40
  • EUR 319.20
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17288 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17289 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17290 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17291 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17292 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17293 5 mL
EUR 308

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-100P 100 Preps 1.25 ml Ask for price

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FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

Fast Probe Master Mix with Rox, trial size (100 rxn)
31016-T 1mL
EUR 169.2
Description: Minimum order quantity: 1 unit of 1mL

HotTaq Probe qPCR Mix (no ROX)
BT11002 250rxn
EUR 130.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)
BT11102 250rxn
EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

BrightGreen Express 2X qPCR MasterMix-Low ROX
MasterMix-EL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

SensiFAST Lyo-Ready No-ROX Mix, 2x
BIO-11060 10 x 10ml Ask for price

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BIO-11061 5ml Ask for price

Genious 2X SYBRGREEN FASTqPCR MIX (High ROX)
RK21207 1 ml Ask for price

Taq PCR Master Mix (2X, Blue Dye)
BS9295 1ml
EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)
BS9296 5ml
EUR 227.04

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BS9297 1ml
EUR 101.76

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BS9298 5ml
EUR 227.04

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-010 2x50 rxns
EUR 303.6

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P0342-025 5X50 rxns
EUR 546

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P0342-050 10X50 rxns
EUR 934.8

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P0342-100 20x50 rxns
EUR 1599.6

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (500 reactions)
31044-1 1kit
EUR 396
Description: Minimum order quantity: 1 unit of 1kit

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (100 reactions)
31044-T 1kit
EUR 136.8
Description: Minimum order quantity: 1 unit of 1kit

2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn
W0655-1 - Ask for price

2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn
W0655-5 - Ask for price

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions 100 Reactions
EUR 392.4

Bio-Star Mastermix with SYBR (2X) BLUE
Y220 100 rcs (2,5 ml)
EUR 112.8

Accuris qMax Probe No Rox qPCR Mix
PR2001-N-1000 1 PC
EUR 561.29

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-001 1ml
EUR 175.2

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-002 2x1ml
EUR 248.4

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-005 5x1ml
EUR 319.2

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-010 10x1ml
EUR 530.4

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-050 50x1ml
EUR 2004

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-001 1ml
EUR 208.8

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-002 2x1ml
EUR 319.2

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-005 5x1ml
EUR 562.8

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-010 10x1ml
EUR 886.8

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-050 50x1ml
EUR 3462

2xEs Taq Master Mix (with Dyes)
W0690-1 - Ask for price

2xEs Taq Master Mix (with Dyes)
W0690-5 - Ask for price

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500
HS-100-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500
HS-150-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials
S210 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials
S210L 500 rcs (12,5 ml)
EUR 627.6

Bio-Star Mastermix with SYBR (2X) no dye
Y210 100 rcs (2,5 ml)
EUR 106.8

SYBR Green I Nucleic Acid Gel Stain
HY-K1004 100 μL
EUR 135.6

Fast EvaGreen Master Mix for qPCR(200 rxn)
31003 2x1ML
EUR 244.8
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)
31003-1 5x1ML
EUR 463.2
Description: Minimum order quantity: 1 unit of 5x1ML

Fast EvaGreen Master Mix for qPCR (5000 rxn)
31003-2 50x1ML
EUR 3739.2
Description: Minimum order quantity: 1 unit of 50x1ML

Fast Plus EvaGreen qPCR Master Mix (200 rxn)
31020 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix (500 rxn)
31020-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)
31020-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Genorise® 2 x qPCR HotStart Master Mix
GR108002 2.0 ml
EUR 334.8

RT Master Mix for qPCR (gDNA digester plus)
HY-K0511 1 mL (100 rxns)
EUR 378

Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn)
QPCR001-R 4X1.25ml
EUR 303.25

ABScript II One Step SYBR Green RT-qPCR Kit
RK20404 10 RXN Ask for price

Deoxy+ SYBR Green OneStep qRT-PCRKit wi ROX, 100 preps
FYT504-100P 1 vial Ask for price

Deoxy+ SYBR Green OneStep qRT-PCRwi ROX Kit, 50 preps
FYT504-50P 1 vial Ask for price

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
S170 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
S170L 500 rcs (12,5 ml)
EUR 627.6

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S175 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S175L 500 rcs (12,5 ml)
EUR 627.6

qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S215 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S215L 500 rcs (12,5 ml)
EUR 627.6

qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S220 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S220L 500 rcs (12,5 ml)
EUR 734.4

qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials
S225 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials
S225L 500 rcs (12,5 ml)
EUR 734.4

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-372L 10 x 1,25 ml
EUR 720.29
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-372S 2 x 1,25 ml
EUR 180.14
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

Jade? Mix-ROX
M1108-500
EUR 529.2

2×Taq PCR Master Mix(with dye)
K1034-1 1 ml
EUR 87.6

2×Taq PCR Master Mix(with dye)
K1034-100 100×1 ml
EUR 950.4

2×Taq PCR Master Mix(with dye)
K1034-20 20×1 ml
EUR 338.4

2×Taq PCR Master Mix(with dye)
K1034-5 5×1 ml
EUR 142.8

2×Taq PCR Master Mix(with dye)
K1034-50 50×1 ml
EUR 616.8

ACCUZYME Mix, 2x
BIO-25028 500 Reactions Ask for price

MyTaq Mix, 2x
BIO-25041 200 Reactions Ask for price
PANDAA-quantified DRMs current at frequency ≥5% (2 h from nucleic acid to consequence) with a sensitivity and specificity of 96.9% and 97.5%, respectively. PANDAA is an revolutionary development with applicability to any pathogen the place target-proximal genetic variability hinders diagnostic growth.
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