Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene
An correct genetic diagnostic is essential for enough affected person administration and the suitability of healthcare techniques. The scientific problem lies in growing strategies to discriminate these sufferers with sure genetic variations current in tumor cells at low concentrations. We report a technique referred to as enhanced uneven blocked qPCR (EAB-qPCR) that promotes the blocker annealing towards the primer-template hybrid controlling thermal biking and response circumstances with nonmodified oligonucleotides. Actual-time fluorescent amplification curves of wild-type alleles had been delayed by about eight cycles for EAB-qPCR, in comparison with standard blocked qPCR approaches.
This methodology diminished the amplification of native DNA variants (blocking proportion 99.7%) and enabled the efficient enrichment of low-level DNA mutations. Wonderful efficiency was estimated for the detection of mutated alleles in sensitivity (as much as 0.5% mutant/whole DNA) and reproducibility phrases, with a relative commonplace deviation under 2.8%. The strategy was efficiently utilized to the mutational evaluation of metastatic colorectal carcinoma from biopsied tissues.
The decided single-nucleotide mutations within the KRAS oncogene (codon 12-13) completely agreed with these obtained from next-generation sequencing. EAB-qPCR is an correct low cost methodology and might be simply included into day by day routine to detect mutant alleles. Therefore, these options are particularly attention-grabbing to facilitate the analysis and prognosis of a number of scientific ailments.
Quantification of Actaea racemosa L. (black cohosh) from a few of its potential adulterants utilizing qPCR and dPCR strategies
The demand for well-liked pure well being merchandise (NHPs) similar to Black Cohosh is growing significantly, which in flip challenges high quality assurance (QA) all through the availability chain. To detect and quantify the goal species current in a given NHP, DNA-based molecular strategies similar to Actual-time quantitative PCR (qPCR) and digital PCR (dPCR) are commonplace instruments within the meals and pathogen testing industries.
There’s a hole within the literature regarding validated quantitative PCR strategies for botanicals that may be utilized for QA and good manufacturing practices. The target of this research is to develop an environment friendly quantification methodology utilizing qPCR and dPCR strategies for the detection and quantification of Actaea racemosa (Black cohosh) NHPs from its potential adulterants. These developed strategies are validated for applicability on business NHPs.
Species-specific hydrolysis probe assays had been designed to research the black cohosh NHPs utilizing qPCR and dPCR strategies. The outcomes confirmed that the developed qPCR and dPCR strategies are extremely exact for figuring out and quantifying black cohosh NHPs, indicating their potential applicability in future routine industrial and laboratory testing. This permits a single qPCR check to find out not solely the presence of a particular botanical, but additionally the quantity when combined with an adulterant.
Quantification of Legionella pneumophila by qPCR and tradition in faucet water with completely different concentrations of residual disinfectants and heterotrophic micro organism
Legionellosis prevalence is growing in the USA. This illness is precipitated primarily by the bacterium Legionella pneumophila present in water and transmitted by aerosol inhalation. This pathogen has a gradual progress fee and may “disguise” in amoeba, making it tough to watch by the normal tradition methodology on selective media. Faucet water samples (n = 358) collected throughout the USA had been examined for L. pneumophila by each tradition and quantitative Polymerase Chain Response (qPCR).
The presence of different micro organism was quantified by heterotrophic plate counts (HPC). Residual disinfectant concentrations (free chlorine or monochloramine) had been measured in all samples. Legionella pneumophila had the best prevalence and focus within the chlorinated water samples that had a free‑chlorine worth of lower than 0.2 mg Cl2/L. In whole, 24% (87/358) of the samples had been constructive for L. pneumophila both by qPCR or 3% (11/358) had been constructive by tradition.

In chloramine-treated samples, L. pneumophila was detected by qPCR in 21% (31/148) and 1% (2/148) by tradition, regardless of a excessive monochloramine residual >1 mg Cl2/L. Regardless of the presence of a excessive disinfectant residual (>1 mg Cl2/L), HPC counts had been substantial. This research signifies that each tradition and qPCR strategies have limitations when predicting a possible danger for illness related to L. pneumophila in faucet water. Measuring disinfectant residuals and quantifying HPC in water samples could also be helpful adjunct parameters for lowering Legionellosis’ danger from public water provides at high-risk areas.
PANDAA deliberately violates standard qPCR design to allow sturdy, mismatch-agnostic detection of extremely polymorphic pathogens
Delicate and reproducible diagnostics are elementary to containing the unfold of present and rising pathogens. Regardless of the reliance of scientific virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding areas produces false-negative outcomes.
We systematically violated canonical qPCR design rules to develop a Pan-Degenerate Amplification and Adaptation (PANDAA), a degree mutation assay that mitigates the influence of sequence variation on probe-based qPCR efficiency. Utilizing HIV-1 as a mannequin system, we optimized and validated PANDAA to detect HIV drug resistance mutations (DRMs). Extremely-degenerate primers with 3′ termini overlapping the probe-binding web site adapt the goal by site-directed mutagenesis throughout qPCR to exchange DRM-proximal sequence variation.
SYBR Green qPCR Master Mix (No ROX) |
HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
SYBR Green qPCR Master Mix (Low ROX) |
HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
SYBR Green qPCR Master Mix (High ROX) |
HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 135.6 |
2x SYBR Green qPCR Master Mix |
B21202 |
Bimake |
5 mL |
EUR 268.8 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2x SYBR Green qPCR Master Mix |
B21203 |
Bimake |
25 mL |
EUR 1027.2 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
SYBR Green qPCR Master Mix |
HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 315.6 |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-25 |
ApexBio |
25ml |
EUR 420 |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-5 |
ApexBio |
5ml |
EUR 120 |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-50 |
ApexBio |
50ml |
EUR 840 |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-25 |
ApexBio |
25ml |
EUR 420 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-5 |
ApexBio |
5ml |
EUR 120 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-50 |
ApexBio |
50ml |
EUR 840 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
AceQ qPCR SYBR® Green Master Mix |
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
Q321-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
Q321-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
Q421-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
Q421-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17272-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17273-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17274-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17275-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17276-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17277-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17278-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17279-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17280-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17281-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17270 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17271 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17272 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17273 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17274 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17275 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17276 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17277 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17278 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17279 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17280 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17281 |
AAT Bioquest |
5 mL |
EUR 308 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
Q331-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
Q331-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
Q341-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
Q341-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
RK21205 |
Abclonal |
1 ml |
Ask for price |
|
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
Q431-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
Q431-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
Q441-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
Q441-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
ChamQ SYBR qPCR Master Mix |
Q311-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix |
Q311-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
ChamQ SYBR Color qPCR Master Mix |
Q411-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix |
Q411-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
AceQ Universal SYBR qPCR Master Mix |
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ Universal SYBR qPCR Master Mix |
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
Genious 2X SYBR Green Fast qPCR Mix |
RK21204 |
Abclonal |
1 mL |
Ask for price |
|
2X Universal SYBR Green Fast qPCR Mix |
RK21203 |
Abclonal |
1 mL |
Ask for price |
|
iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul |
A4183-4x125ML500Rxns |
Biomatik Corporation |
4x1.25ML (500Rxns) |
EUR 200.2 |
Description: Biotechnology |
BrightCycle Universal SYBR Green qPCR Mix with UDG |
RK21219 |
Abclonal |
5mL |
EUR 475 |
Taq Pro Universal SYBR qPCR Master Mix |
Q712-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 128.8 |
Taq Pro Universal SYBR qPCR Master Mix |
Q712-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 580 |
miRNA Universal SYBR® qPCR Master Mix |
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 165.6 |
miRNA Universal SYBR® qPCR Master Mix |
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 290.4 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173L |
MiTeGen |
960 reactions x 20 µl |
EUR 897 |
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173S |
MiTeGen |
192 reactions x 20 µl |
EUR 224 |
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
31014-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
31015-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
31014-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
31015-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
31042-1 |
Biotium |
5x1mL |
EUR 326.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
31042-20mL |
Biotium |
2x10mL |
EUR 592.8 |
Description: Minimum order quantity: 1 unit of 2x10mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
31042-T |
Biotium |
1mL |
EUR 136.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Entrans 2X qPCR Probe Master Mix with UDG |
RK21222 |
Abclonal |
50RXN |
EUR 71 |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
31014-T |
Biotium |
1mL |
EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
31015-T |
Biotium |
1mL |
EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
31014 |
Biotium |
2x1mL |
EUR 273.6 |
Description: Minimum order quantity: 1 unit of 2x1mL |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-001 |
GenDepot |
1ml |
EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-002 |
GenDepot |
2x1ml |
EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-005 |
GenDepot |
5x1ml |
EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-010 |
GenDepot |
10x1ml |
EUR 1632 |
Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
31015 |
Biotium |
2x1mL |
EUR 273.6 |
Description: Minimum order quantity: 1 unit of 2x1mL |
HotStart PCR Master mix (2X, Green Dye) |
BS9291 |
Bio Basic |
5X1mL, 5ml |
EUR 251.4 |
|
HotStart PCR Master mix (2X, Green Dye) |
BS9292 |
Bio Basic |
1ml |
EUR 115.68 |
|
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-001 |
GenDepot |
1ml |
EUR 175.2 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-002 |
GenDepot |
2x1ml |
EUR 248.4 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-005 |
GenDepot |
5x1ml |
EUR 322.8 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-010 |
GenDepot |
10x1ml |
EUR 537.6 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-050 |
GenDepot |
50x1ml |
EUR 2085.6 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-001 |
GenDepot |
1ml |
EUR 208.8 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-002 |
GenDepot |
2x1ml |
EUR 319.2 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-005 |
GenDepot |
5x1ml |
EUR 562.8 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-010 |
GenDepot |
10x1ml |
EUR 886.8 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-050 |
GenDepot |
50x1ml |
EUR 3462 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-001 |
GenDepot |
1ml |
EUR 175.2 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-002 |
GenDepot |
2x1ml |
EUR 248.4 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-005 |
GenDepot |
5x1ml |
EUR 319.2 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-010 |
GenDepot |
10x1ml |
EUR 530.4 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-050 |
GenDepot |
50x1ml |
EUR 2004 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-001 |
GenDepot |
1ml |
EUR 208.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-002 |
GenDepot |
2x1ml |
EUR 319.2 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-003 |
GenDepot |
5x1ml |
EUR 562.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-004 |
GenDepot |
10x1ml |
EUR 886.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-005 |
GenDepot |
50x1ml |
EUR 3462 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-001 |
GenDepot |
1ml |
EUR 175.2 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-002 |
GenDepot |
2x1ml |
EUR 248.4 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-005 |
GenDepot |
5x1ml |
EUR 319.2 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-010 |
GenDepot |
10x1ml |
EUR 530.4 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-050 |
GenDepot |
50x1ml |
EUR 2004 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-001 |
GenDepot |
1ml |
EUR 208.8 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-002 |
GenDepot |
2x1ml |
EUR 319.2 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-005 |
GenDepot |
5x1ml |
EUR 562.8 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-010 |
GenDepot |
10x1ml |
EUR 886.8 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-050 |
GenDepot |
50x1ml |
EUR 400.8 |
Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
QPCR002-L |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Entrans 2X qPCR Probe Master Mix |
RK21208 |
Abclonal |
40 RXN |
Ask for price |
|
1-Step 2X RT-PCR Master Mix-SYBR Green-HY (High Yield) |
W141 |
101Bio |
200 rxn |
EUR 199 |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
17282-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
17283-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
17284-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
17285-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
EZGreen 2x qPCR Master Mix, 40Rxns-50ul |
A4157-1ML40Rxns |
Biomatik Corporation |
1ML (40Rxns) |
EUR 55 |
Description: Biotechnology |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
17286-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
17287-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
MB1004-100Reactions |
BosterBio |
100 Reactions |
EUR 392.4 |
Fast Probe Master Mix with Rox (5000 rxn) |
31016-2 |
Biotium |
50x1mL |
EUR 4645.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Bio-Star Mastermix with SYBR (2X) Low-ROX |
Y250 |
GeneOn |
100 rcs (2,5 ml) |
EUR 122.4 |
1-Step 2X RT-PCR Master Mix-SYBR Green-HP (High Processibility) |
W142 |
101Bio |
200 rxn |
EUR 199 |
Entrans 2X qPCR Probe Master Mix V2 |
RK21212 |
Abclonal |
50RXN |
EUR 67.5 |
Bio-Star Mastermix with SYBR (2X) High-ROX |
Y240 |
GeneOn |
100 rcs (2,5 ml) |
EUR 122.4 |
Fast Probe Master Mix with Rox (200 rxn) |
31016 |
Biotium |
2x1mL |
EUR 280.8 |
Description: Minimum order quantity: 1 unit of 2x1mL |
Fast Probe Master Mix with Rox (500 rxn) |
31016-1 |
Biotium |
5x1mL |
EUR 559.2 |
Description: Minimum order quantity: 1 unit of 5x1mL |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17282 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17283 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17284 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17285 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17286 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17287 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
QPCR001-R |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291 |
AAT Bioquest |
5 mL |
EUR 308 |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
9-31045 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293 |
AAT Bioquest |
5 mL |
EUR 308 |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
9-31046 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 349.2 |
TaqProbe 2X qPCR MasterMix-Low ROX |
MasterMix-PL |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
S210 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
S210L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
KiloGreen 2X qPCR MasterMix-Low ROX |
MasterMix-KL |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 168 |
AceQ qPCR Probe Master Mix |
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR Probe Master Mix |
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
Fast Probe Master Mix with Rox, trial size (100 rxn) |
31016-T |
Biotium |
1mL |
EUR 169.2 |
Description: Minimum order quantity: 1 unit of 1mL |
ABScript III RT Master Mix for qPCR with gDNA Remover |
RK20429 |
Abclonal |
10RXN |
EUR 20 |
ABScript Neo RT Master Mix for qPCR with gDNA Remover |
RK20433 |
Abclonal |
100RXN |
EUR 30 |
BrightGreen 2X qPCR MasterMix-Low ROX |
MasterMix-LR |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
BrightGreen 2X qPCR MasterMix-Low ROX |
MasterMix-LR-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 451.2 |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
S220 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
S220L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
S225 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
S225L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
S170 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
S170L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX) |
RK21206 |
Abclonal |
1 ml |
Ask for price |
|
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S175 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S175L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
Bio-Star Mastermix with SYBR (2X) BLUE |
Y220 |
GeneOn |
100 rcs (2,5 ml) |
EUR 112.8 |
Gloria Nova HS 2X Master Mix with Dye |
RK20728 |
Abclonal |
1mL |
EUR 107.5 |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S215 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S215L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
TaqProbe 2X qPCR MasterMix-ROX |
MasterMix-P |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
KiloGreen 2X qPCR MasterMix-ROX |
MasterMix-KR |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 168 |
Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (500 reactions) |
31044-1 |
Biotium |
1kit |
EUR 396 |
Description: Minimum order quantity: 1 unit of 1kit |
Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (100 reactions) |
31044-T |
Biotium |
1kit |
EUR 136.8 |
Description: Minimum order quantity: 1 unit of 1kit |
BrightGreen 2X qPCR MasterMix-ROX |
MasterMix-R |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
BrightGreen 2X qPCR MasterMix-ROX |
MasterMix-R-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 451.2 |
qPCR Master Mix E4 (SafeLine: EvaGreen, dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
S180 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix E4 (SafeLine: EvaGreen, dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
S180L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials |
S185 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials |
S185L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
BrightGreen Express 2X qPCR MasterMix-Low ROX |
MasterMix-EL |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
31020-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Bio-Star Mastermix with SYBR (2X) no dye |
Y210 |
GeneOn |
100 rcs (2,5 ml) |
EUR 106.8 |
Accuris qMax Green, No Rox qPCR Mix, 1000 reactions |
PR2000-N-1000 |
Benchmark Scientific |
1 PC |
EUR 623.8 |
Accuris qMax Green, Low Rox qPCR Mix, 1000 reactions |
PR2000-L-1000 |
Benchmark Scientific |
1 PC |
EUR 623.8 |
Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
31020-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Taqman Master Mix-Low ROX |
M1123-500 |
Biovision |
each |
EUR 529.2 |
Accuris qMax Green, High Rox qPCR Mix, 1000 reactions |
PR2000-H-1000 |
Benchmark Scientific |
1 PC |
EUR 623.8 |
PANDAA-quantified DRMs current at frequency ≥5% (2 h from nucleic acid to consequence) with a sensitivity and specificity of 96.9% and 97.5%, respectively. PANDAA is an revolutionary development with applicability to any pathogen the place target-proximal genetic variability hinders diagnostic growth.
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