Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene
An correct genetic diagnostic is essential for enough affected person administration and the suitability of healthcare techniques. The scientific problem lies in growing strategies to discriminate these sufferers with sure genetic variations current in tumor cells at low concentrations. We report a technique referred to as enhanced uneven blocked qPCR (EAB-qPCR) that promotes the blocker annealing towards the primer-template hybrid controlling thermal biking and response circumstances with nonmodified oligonucleotides. Actual-time fluorescent amplification curves of wild-type alleles had been delayed by about eight cycles for EAB-qPCR, in comparison with standard blocked qPCR approaches.
This methodology diminished the amplification of native DNA variants (blocking proportion 99.7%) and enabled the efficient enrichment of low-level DNA mutations. Wonderful efficiency was estimated for the detection of mutated alleles in sensitivity (as much as 0.5% mutant/whole DNA) and reproducibility phrases, with a relative commonplace deviation under 2.8%. The strategy was efficiently utilized to the mutational evaluation of metastatic colorectal carcinoma from biopsied tissues.
The decided single-nucleotide mutations within the KRAS oncogene (codon 12-13) completely agreed with these obtained from next-generation sequencing. EAB-qPCR is an correct low cost methodology and might be simply included into day by day routine to detect mutant alleles. Therefore, these options are particularly attention-grabbing to facilitate the analysis and prognosis of a number of scientific ailments.
Quantification of Actaea racemosa L. (black cohosh) from a few of its potential adulterants utilizing qPCR and dPCR strategies
The demand for well-liked pure well being merchandise (NHPs) similar to Black Cohosh is growing significantly, which in flip challenges high quality assurance (QA) all through the availability chain. To detect and quantify the goal species current in a given NHP, DNA-based molecular strategies similar to Actual-time quantitative PCR (qPCR) and digital PCR (dPCR) are commonplace instruments within the meals and pathogen testing industries.
There’s a hole within the literature regarding validated quantitative PCR strategies for botanicals that may be utilized for QA and good manufacturing practices. The target of this research is to develop an environment friendly quantification methodology utilizing qPCR and dPCR strategies for the detection and quantification of Actaea racemosa (Black cohosh) NHPs from its potential adulterants. These developed strategies are validated for applicability on business NHPs.
Species-specific hydrolysis probe assays had been designed to research the black cohosh NHPs utilizing qPCR and dPCR strategies. The outcomes confirmed that the developed qPCR and dPCR strategies are extremely exact for figuring out and quantifying black cohosh NHPs, indicating their potential applicability in future routine industrial and laboratory testing. This permits a single qPCR check to find out not solely the presence of a particular botanical, but additionally the quantity when combined with an adulterant.
Quantification of Legionella pneumophila by qPCR and tradition in faucet water with completely different concentrations of residual disinfectants and heterotrophic micro organism
Legionellosis prevalence is growing in the USA. This illness is precipitated primarily by the bacterium Legionella pneumophila present in water and transmitted by aerosol inhalation. This pathogen has a gradual progress fee and may “disguise” in amoeba, making it tough to watch by the normal tradition methodology on selective media. Faucet water samples (n = 358) collected throughout the USA had been examined for L. pneumophila by each tradition and quantitative Polymerase Chain Response (qPCR).
The presence of different micro organism was quantified by heterotrophic plate counts (HPC). Residual disinfectant concentrations (free chlorine or monochloramine) had been measured in all samples. Legionella pneumophila had the best prevalence and focus within the chlorinated water samples that had a free‑chlorine worth of lower than 0.2 mg Cl2/L. In whole, 24% (87/358) of the samples had been constructive for L. pneumophila both by qPCR or 3% (11/358) had been constructive by tradition.

In chloramine-treated samples, L. pneumophila was detected by qPCR in 21% (31/148) and 1% (2/148) by tradition, regardless of a excessive monochloramine residual >1 mg Cl2/L. Regardless of the presence of a excessive disinfectant residual (>1 mg Cl2/L), HPC counts had been substantial. This research signifies that each tradition and qPCR strategies have limitations when predicting a possible danger for illness related to L. pneumophila in faucet water. Measuring disinfectant residuals and quantifying HPC in water samples could also be helpful adjunct parameters for lowering Legionellosis’ danger from public water provides at high-risk areas.
PANDAA deliberately violates standard qPCR design to allow sturdy, mismatch-agnostic detection of extremely polymorphic pathogens
Delicate and reproducible diagnostics are elementary to containing the unfold of present and rising pathogens. Regardless of the reliance of scientific virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding areas produces false-negative outcomes.
We systematically violated canonical qPCR design rules to develop a Pan-Degenerate Amplification and Adaptation (PANDAA), a degree mutation assay that mitigates the influence of sequence variation on probe-based qPCR efficiency. Utilizing HIV-1 as a mannequin system, we optimized and validated PANDAA to detect HIV drug resistance mutations (DRMs). Extremely-degenerate primers with 3′ termini overlapping the probe-binding web site adapt the goal by site-directed mutagenesis throughout qPCR to exchange DRM-proximal sequence variation.
SYBR Green qPCR Master Mix (High ROX) |
HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 135.6 |
SYBR Green qPCR Master Mix (Low ROX) |
HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
SYBR Green qPCR Master Mix (No ROX) |
HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
SYBR Green qPCR Master Mix |
HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 315.6 |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
RK21205 |
Abclonal |
1 ml |
Ask for price |
|
2X Universal SYBR Green Fast qPCR Mix |
RK21203 |
Abclonal |
1 mL |
Ask for price |
|
Genious 2X SYBR Green Fast qPCR Mix |
RK21204 |
Abclonal |
1 mL |
Ask for price |
|
AceQ qPCR SYBR® Green Master Mix |
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17270 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17271 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17272 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17273 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17274 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17275 |
AAT Bioquest |
5 mL |
EUR 308 |
AceQ Universal SYBR qPCR Master Mix |
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ Universal SYBR qPCR Master Mix |
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17276 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17277 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17278 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17279 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17280 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17281 |
AAT Bioquest |
5 mL |
EUR 308 |
Entrans 2X qPCR Probe Master Mix |
RK21208 |
Abclonal |
40 RXN |
Ask for price |
|
miRNA Universal SYBR® qPCR Master Mix |
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 165.6 |
miRNA Universal SYBR® qPCR Master Mix |
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 290.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-001 |
GenDepot |
1ml |
EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-002 |
GenDepot |
2x1ml |
EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-005 |
GenDepot |
5x1ml |
EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-010 |
GenDepot |
10x1ml |
EUR 1632 |
HotStart PCR Master mix (2X, Green Dye) |
BS9291 |
Bio Basic |
5X1mL, 5ml |
EUR 251.4 |
|
HotStart PCR Master mix (2X, Green Dye) |
BS9292 |
Bio Basic |
1ml |
EUR 115.68 |
|
Bio-Star Mastermix with SYBR (2X) High-ROX |
Y240 |
GeneOn |
100 rcs (2,5 ml) |
EUR 122.4 |
Bio-Star Mastermix with SYBR (2X) Low-ROX |
Y250 |
GeneOn |
100 rcs (2,5 ml) |
EUR 122.4 |
Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX) |
RK21206 |
Abclonal |
1 ml |
Ask for price |
|
Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
31014 |
Biotium |
2x1mL |
EUR 273.6 |
Description: Minimum order quantity: 1 unit of 2x1mL |
Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
31014-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
31014-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
31015-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
31015-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
31042-1 |
Biotium |
5x1mL |
EUR 326.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
31042-20mL |
Biotium |
2x10mL |
EUR 592.8 |
Description: Minimum order quantity: 1 unit of 2x10mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
31042-T |
Biotium |
1mL |
EUR 136.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Taqman Master Mix-ROX |
M1124-500 |
Biovision |
each |
EUR 529.2 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-001 |
GenDepot |
1ml |
EUR 175.2 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-002 |
GenDepot |
2x1ml |
EUR 248.4 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-005 |
GenDepot |
5x1ml |
EUR 322.8 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-010 |
GenDepot |
10x1ml |
EUR 537.6 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-050 |
GenDepot |
50x1ml |
EUR 2085.6 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-001 |
GenDepot |
1ml |
EUR 175.2 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-002 |
GenDepot |
2x1ml |
EUR 248.4 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-005 |
GenDepot |
5x1ml |
EUR 319.2 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-010 |
GenDepot |
10x1ml |
EUR 530.4 |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
Q5602-050 |
GenDepot |
50x1ml |
EUR 2004 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-001 |
GenDepot |
1ml |
EUR 175.2 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-002 |
GenDepot |
2x1ml |
EUR 248.4 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-005 |
GenDepot |
5x1ml |
EUR 319.2 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-010 |
GenDepot |
10x1ml |
EUR 530.4 |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
Q5603-050 |
GenDepot |
50x1ml |
EUR 2004 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-001 |
GenDepot |
1ml |
EUR 208.8 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-002 |
GenDepot |
2x1ml |
EUR 319.2 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-005 |
GenDepot |
5x1ml |
EUR 562.8 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-010 |
GenDepot |
10x1ml |
EUR 886.8 |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
Q5700-050 |
GenDepot |
50x1ml |
EUR 3462 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-001 |
GenDepot |
1ml |
EUR 208.8 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-002 |
GenDepot |
2x1ml |
EUR 319.2 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-005 |
GenDepot |
5x1ml |
EUR 562.8 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-010 |
GenDepot |
10x1ml |
EUR 886.8 |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
Q5702-050 |
GenDepot |
50x1ml |
EUR 400.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-001 |
GenDepot |
1ml |
EUR 208.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-002 |
GenDepot |
2x1ml |
EUR 319.2 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-003 |
GenDepot |
5x1ml |
EUR 562.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-004 |
GenDepot |
10x1ml |
EUR 886.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
Q5703-005 |
GenDepot |
50x1ml |
EUR 3462 |
SYBR Green I |
B1747-25 |
Biovision |
each |
EUR 1396.8 |
SYBR Green I |
B1747-5 |
Biovision |
each |
EUR 464.4 |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
31014-T |
Biotium |
1mL |
EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
31015 |
Biotium |
2x1mL |
EUR 273.6 |
Description: Minimum order quantity: 1 unit of 2x1mL |
Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
31015-T |
Biotium |
1mL |
EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Fast Probe Master Mix with Rox (200 rxn) |
31016 |
Biotium |
2x1mL |
EUR 280.8 |
Description: Minimum order quantity: 1 unit of 2x1mL |
Fast Probe Master Mix with Rox (500 rxn) |
31016-1 |
Biotium |
5x1mL |
EUR 559.2 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Probe Master Mix with Rox (5000 rxn) |
31016-2 |
Biotium |
50x1mL |
EUR 4645.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
KiloGreen 2X qPCR MasterMix-ROX |
MasterMix-KR |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 168 |
TaqProbe 2X qPCR MasterMix-ROX |
MasterMix-P |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
BrightGreen 2X qPCR MasterMix-ROX |
MasterMix-R |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
BrightGreen 2X qPCR MasterMix-ROX |
MasterMix-R-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 451.2 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173L |
MiTeGen |
960 reactions x 20 µl |
EUR 789.36 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173S |
MiTeGen |
192 reactions x 20 µl |
EUR 197.34 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
Taqman Master Mix-Low ROX |
M1123-500 |
Biovision |
each |
EUR 529.2 |
EZSolution? SYBR Green I |
B1748-1 |
Biovision |
each |
EUR 744 |
EZSolution? SYBR Green I |
B1748-500 |
Biovision |
each |
EUR 516 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17282 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17283 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17284 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17285 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17286 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17287 |
AAT Bioquest |
5 mL |
EUR 308 |
HotTaq Probe qPCR Mix (ROX) |
BT11001 |
Bioatlas |
250rxn |
EUR 130.8 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Mix (ROX) |
BT11101 |
Bioatlas |
250rxn |
EUR 147.6 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 349.2 |
AceQ qPCR Probe Master Mix |
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR Probe Master Mix |
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
BrightGreen Express 2X qPCR MasterMix-ROX |
MasterMix-ER |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
KiloGreen 2X qPCR MasterMix-Low ROX |
MasterMix-KL |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 168 |
BrightGreen 2X qPCR MasterMix-Low ROX |
MasterMix-LR |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
BrightGreen 2X qPCR MasterMix-Low ROX |
MasterMix-LR-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 451.2 |
TaqProbe 2X qPCR MasterMix-Low ROX |
MasterMix-PL |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
amfiSure ONE PCR Master Mix(2X) |
P7000-005 |
GenDepot |
5x1 ml |
EUR 262.8 |
amfiSure ONE PCR Master Mix(2X) |
P7000-010 |
GenDepot |
10x1 ml |
EUR 380.4 |
amfiSure ONE PCR Master Mix(2X) |
P7000-050 |
GenDepot |
50x1 ml |
EUR 1453.2 |
amfiSure ONE PCR Master Mix(2X) |
P7000-100 |
GenDepot |
100x1 ml |
EUR 2522.4 |
Gloria Nova HS 2x Master Mix |
RK20717 |
Abclonal |
1mL |
Ask for price |
|
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
9-31045 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
9-31046 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293 |
AAT Bioquest |
5 mL |
EUR 308 |
SensiFAST SYBR Lo-ROX Mix, 50 mL |
BIO-94050 |
Bioline |
5000 rxn |
Ask for price |
SensiFAST SYBR No-ROX Mix, 50 mL |
BIO-98050 |
Bioline |
5000 rxn |
Ask for price |
Eztime Real - time PCRPremix 2x,SYBR Green |
FYT103-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
Ask for price |
Eztime Real - time PCRPremix 2x,SYBR Green |
FYT103-400P |
Yeastern Biotech |
400 Preps 1.25 ml x 4 |
Ask for price |
Fast Probe Master Mix with Rox, trial size (100 rxn) |
31016-T |
Biotium |
1mL |
EUR 169.2 |
Description: Minimum order quantity: 1 unit of 1mL |
HotTaq Probe qPCR Mix (no ROX) |
BT11002 |
Bioatlas |
250rxn |
EUR 130.8 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Mix (no ROX) |
BT11102 |
Bioatlas |
250rxn |
EUR 147.6 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
BrightGreen Express 2X qPCR MasterMix-Low ROX |
MasterMix-EL |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
SensiFAST Lyo-Ready No-ROX Mix, 2x |
BIO-11060 |
Cusabio |
10 x 10ml |
Ask for price |
SensiFAST Lyo-Ready No-ROX Mix, 2x |
BIO-11061 |
Bioline |
5ml |
Ask for price |
Genious 2X SYBRGREEN FASTqPCR MIX (High ROX) |
RK21207 |
Abclonal |
1 ml |
Ask for price |
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9295 |
Bio Basic |
1ml |
EUR 101.76 |
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9296 |
Bio Basic |
5ml |
EUR 227.04 |
|
Taq PCR Master Mix (2X, Red Dye) |
BS9297 |
Bio Basic |
1ml |
EUR 101.76 |
|
Taq PCR Master Mix (2X, Red Dye) |
BS9298 |
Bio Basic |
5ml |
EUR 227.04 |
|
amfiFusion High Fidelity PCR Master Mix(2X) |
P0342-010 |
GenDepot |
2x50 rxns |
EUR 303.6 |
amfiFusion High Fidelity PCR Master Mix(2X) |
P0342-025 |
GenDepot |
5X50 rxns |
EUR 546 |
amfiFusion High Fidelity PCR Master Mix(2X) |
P0342-050 |
GenDepot |
10X50 rxns |
EUR 934.8 |
amfiFusion High Fidelity PCR Master Mix(2X) |
P0342-100 |
GenDepot |
20x50 rxns |
EUR 1599.6 |
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
MB1004-100Reactions |
BosterBio |
100 Reactions |
EUR 392.4 |
Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (500 reactions) |
31044-1 |
Biotium |
1kit |
EUR 396 |
Description: Minimum order quantity: 1 unit of 1kit |
Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (100 reactions) |
31044-T |
Biotium |
1kit |
EUR 136.8 |
Description: Minimum order quantity: 1 unit of 1kit |
Bio-Star Mastermix with SYBR (2X) BLUE |
Y220 |
GeneOn |
100 rcs (2,5 ml) |
EUR 112.8 |
Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml) |
PR2120-N-25BLK |
Benchmark Scientific |
1 each |
EUR 2695 |
Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml) |
PR2120-N-50BLK |
Benchmark Scientific |
1 each |
EUR 4785 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
HS-100-QPCR |
CORNING |
100/pk |
EUR 381.6 |
Description: Sealing Products; Sealing films - Axygen |
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
HS-150-QPCR |
CORNING |
100/pk |
EUR 381.6 |
Description: Sealing Products; Sealing films - Axygen |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
Q5601-001 |
GenDepot |
1ml |
EUR 175.2 |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
Q5601-002 |
GenDepot |
2x1ml |
EUR 248.4 |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
Q5601-005 |
GenDepot |
5x1ml |
EUR 319.2 |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
Q5601-010 |
GenDepot |
10x1ml |
EUR 530.4 |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
Q5601-050 |
GenDepot |
50x1ml |
EUR 2004 |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
Q5701-001 |
GenDepot |
1ml |
EUR 208.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
Q5701-002 |
GenDepot |
2x1ml |
EUR 319.2 |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
Q5701-005 |
GenDepot |
5x1ml |
EUR 562.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
Q5701-010 |
GenDepot |
10x1ml |
EUR 886.8 |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
Q5701-050 |
GenDepot |
50x1ml |
EUR 3462 |
Accuris qMAX SYBR Green, No ROX, 2500 reactions (1x25ml) |
PR2000-N-25BLK |
Benchmark Scientific |
1 each |
EUR 1133 |
Accuris qMAX SYBR Green, No ROX, 5000 reactions (1x50ml) |
PR2000-N-50BLK |
Benchmark Scientific |
1 each |
EUR 2039.4 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
S210 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
S210L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
SYBR Green I Nucleic Acid Gel Stain |
HY-K1004 |
MedChemExpress |
100 μL |
EUR 135.6 |
Bio-Star Mastermix with SYBR (2X) no dye |
Y210 |
GeneOn |
100 rcs (2,5 ml) |
EUR 106.8 |
Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
QPCR001-R |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Fast EvaGreen Master Mix for qPCR(200 rxn) |
31003 |
Biotium |
2x1ML |
EUR 244.8 |
Description: Minimum order quantity: 1 unit of 2x1ML |
Fast EvaGreen Master Mix for qPCR (500 rxn) |
31003-1 |
Biotium |
5x1ML |
EUR 463.2 |
Description: Minimum order quantity: 1 unit of 5x1ML |
Fast EvaGreen Master Mix for qPCR (5000 rxn) |
31003-2 |
Biotium |
50x1ML |
EUR 3739.2 |
Description: Minimum order quantity: 1 unit of 50x1ML |
Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
31020-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
31020-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
RT Master Mix for qPCR (gDNA digester plus) |
HY-K0511 |
MedChemExpress |
1 mL (100 rxns) |
EUR 378 |
ABScript II One Step SYBR Green RT-qPCR Kit |
RK20404 |
Abclonal |
10 RXN |
Ask for price |
|
Deoxy+ SYBR Green OneStep qRT-PCRKit wi ROX,
100 preps |
FYT504-100P |
Yeastern Biotech |
1 vial |
Ask for price |
Deoxy+ SYBR Green OneStep qRT-PCRwi ROX Kit,
50 preps |
FYT504-50P |
Yeastern Biotech |
1 vial |
Ask for price |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
S170 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
S170L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S175 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S175L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S215 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S215L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
S220 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
S220L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
S225 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
S225L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-372L |
MiTeGen |
10 x 1,25 ml |
EUR 720.29 |
|
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-372S |
MiTeGen |
2 x 1,25 ml |
EUR 180.14 |
|
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
2×Taq PCR Master Mix(with dye) |
K1034-1 |
ApexBio |
1 ml |
EUR 87.6 |
2×Taq PCR Master Mix(with dye) |
K1034-100 |
ApexBio |
100×1 ml |
EUR 950.4 |
2×Taq PCR Master Mix(with dye) |
K1034-20 |
ApexBio |
20×1 ml |
EUR 338.4 |
2×Taq PCR Master Mix(with dye) |
K1034-5 |
ApexBio |
5×1 ml |
EUR 142.8 |
2×Taq PCR Master Mix(with dye) |
K1034-50 |
ApexBio |
50×1 ml |
EUR 616.8 |
Jade? Mix-ROX |
M1108-500 |
Biovision |
each |
EUR 529.2 |
ACCUZYME Mix, 2x |
BIO-25028 |
Bioline |
500 Reactions |
Ask for price |
MyTaq Mix, 2x |
BIO-25041 |
Bioline |
200 Reactions |
Ask for price |
MyTaq Mix, 2x |
BIO-25041/S |
Bioline |
Sample |
Ask for price |
MyTaq Mix, 2x |
BIO-25042 |
Bioline |
1000 Reactions |
Ask for price |
MyFi Mix, 2x |
BIO-25049 |
Bioline |
100 Reactions |
Ask for price |
PANDAA-quantified DRMs current at frequency ≥5% (2 h from nucleic acid to consequence) with a sensitivity and specificity of 96.9% and 97.5%, respectively. PANDAA is an revolutionary development with applicability to any pathogen the place target-proximal genetic variability hinders diagnostic growth.
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