Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene
An correct genetic diagnostic is essential for enough affected person administration and the suitability of healthcare techniques. The scientific problem lies in growing strategies to discriminate these sufferers with sure genetic variations current in tumor cells at low concentrations. We report a technique referred to as enhanced uneven blocked qPCR (EAB-qPCR) that promotes the blocker annealing towards the primer-template hybrid controlling thermal biking and response circumstances with nonmodified oligonucleotides. Actual-time fluorescent amplification curves of wild-type alleles had been delayed by about eight cycles for EAB-qPCR, in comparison with standard blocked qPCR approaches.
This methodology diminished the amplification of native DNA variants (blocking proportion 99.7%) and enabled the efficient enrichment of low-level DNA mutations. Wonderful efficiency was estimated for the detection of mutated alleles in sensitivity (as much as 0.5% mutant/whole DNA) and reproducibility phrases, with a relative commonplace deviation under 2.8%. The strategy was efficiently utilized to the mutational evaluation of metastatic colorectal carcinoma from biopsied tissues.
The decided single-nucleotide mutations within the KRAS oncogene (codon 12-13) completely agreed with these obtained from next-generation sequencing. EAB-qPCR is an correct low cost methodology and might be simply included into day by day routine to detect mutant alleles. Therefore, these options are particularly attention-grabbing to facilitate the analysis and prognosis of a number of scientific ailments.
Quantification of Actaea racemosa L. (black cohosh) from a few of its potential adulterants utilizing qPCR and dPCR strategies
The demand for well-liked pure well being merchandise (NHPs) similar to Black Cohosh is growing significantly, which in flip challenges high quality assurance (QA) all through the availability chain. To detect and quantify the goal species current in a given NHP, DNA-based molecular strategies similar to Actual-time quantitative PCR (qPCR) and digital PCR (dPCR) are commonplace instruments within the meals and pathogen testing industries.
There’s a hole within the literature regarding validated quantitative PCR strategies for botanicals that may be utilized for QA and good manufacturing practices. The target of this research is to develop an environment friendly quantification methodology utilizing qPCR and dPCR strategies for the detection and quantification of Actaea racemosa (Black cohosh) NHPs from its potential adulterants. These developed strategies are validated for applicability on business NHPs.
Species-specific hydrolysis probe assays had been designed to research the black cohosh NHPs utilizing qPCR and dPCR strategies. The outcomes confirmed that the developed qPCR and dPCR strategies are extremely exact for figuring out and quantifying black cohosh NHPs, indicating their potential applicability in future routine industrial and laboratory testing. This permits a single qPCR check to find out not solely the presence of a particular botanical, but additionally the quantity when combined with an adulterant.
Quantification of Legionella pneumophila by qPCR and tradition in faucet water with completely different concentrations of residual disinfectants and heterotrophic micro organism
Legionellosis prevalence is growing in the USA. This illness is precipitated primarily by the bacterium Legionella pneumophila present in water and transmitted by aerosol inhalation. This pathogen has a gradual progress fee and may “disguise” in amoeba, making it tough to watch by the normal tradition methodology on selective media. Faucet water samples (n = 358) collected throughout the USA had been examined for L. pneumophila by each tradition and quantitative Polymerase Chain Response (qPCR).
The presence of different micro organism was quantified by heterotrophic plate counts (HPC). Residual disinfectant concentrations (free chlorine or monochloramine) had been measured in all samples. Legionella pneumophila had the best prevalence and focus within the chlorinated water samples that had a free‑chlorine worth of lower than 0.2 mg Cl2/L. In whole, 24% (87/358) of the samples had been constructive for L. pneumophila both by qPCR or 3% (11/358) had been constructive by tradition.
In chloramine-treated samples, L. pneumophila was detected by qPCR in 21% (31/148) and 1% (2/148) by tradition, regardless of a excessive monochloramine residual >1 mg Cl2/L. Regardless of the presence of a excessive disinfectant residual (>1 mg Cl2/L), HPC counts had been substantial. This research signifies that each tradition and qPCR strategies have limitations when predicting a possible danger for illness related to L. pneumophila in faucet water. Measuring disinfectant residuals and quantifying HPC in water samples could also be helpful adjunct parameters for lowering Legionellosis’ danger from public water provides at high-risk areas.
PANDAA deliberately violates standard qPCR design to allow sturdy, mismatch-agnostic detection of extremely polymorphic pathogens
Delicate and reproducible diagnostics are elementary to containing the unfold of present and rising pathogens. Regardless of the reliance of scientific virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding areas produces false-negative outcomes.
We systematically violated canonical qPCR design rules to develop a Pan-Degenerate Amplification and Adaptation (PANDAA), a degree mutation assay that mitigates the influence of sequence variation on probe-based qPCR efficiency. Utilizing HIV-1 as a mannequin system, we optimized and validated PANDAA to detect HIV drug resistance mutations (DRMs). Extremely-degenerate primers with 3′ termini overlapping the probe-binding web site adapt the goal by site-directed mutagenesis throughout qPCR to exchange DRM-proximal sequence variation.
) SYBR Green qPCR Master Mix (No ROX) |
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C0006-10mg |
TargetMol Chemicals |
10mg |
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Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
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C0006-1g |
TargetMol Chemicals |
1g |
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Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
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C0006-1mg |
TargetMol Chemicals |
1mg |
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Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
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C0006-50mg |
TargetMol Chemicals |
50mg |
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Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
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C0006-5mg |
TargetMol Chemicals |
5mg |
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Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
|
HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
) SYBR Green qPCR Master Mix (Low ROX) |
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C0008-10mg |
TargetMol Chemicals |
10mg |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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C0008-1g |
TargetMol Chemicals |
1g |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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C0008-1mg |
TargetMol Chemicals |
1mg |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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C0008-50mg |
TargetMol Chemicals |
50mg |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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C0008-5mg |
TargetMol Chemicals |
5mg |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
) SYBR Green qPCR Master Mix (High ROX) |
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C0007-10mg |
TargetMol Chemicals |
10mg |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
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C0007-1g |
TargetMol Chemicals |
1g |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
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C0007-1mg |
TargetMol Chemicals |
1mg |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
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C0007-50mg |
TargetMol Chemicals |
50mg |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
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C0007-5mg |
TargetMol Chemicals |
5mg |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
|
HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 135.6 |
 2x SYBR Green qPCR Master Mix |
|
B21202 |
Bimake |
5 mL |
EUR 268.8 |
|
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2x SYBR Green qPCR Master Mix |
|
B21203 |
Bimake |
25 mL |
EUR 1027.2 |
|
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
 ig SYBR Green qPCR 2X Master Mix |
|
3354 |
Intact Genomics |
200 |
EUR 92 |
|
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification. |
 SYBR Green qPCR Master Mix |
|
HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 315.6 |
 Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns |
|
THK0221 |
Westburg |
each |
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 Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns |
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THK0222 |
Westburg |
each |
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 Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns |
|
THK0223 |
Westburg |
each |
EUR 3324.5 |
 AceQ qPCR SYBR® Green Master Mix |
|
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
|
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
 2X SYBR Green Fast qPCR Mix with High ROX |
|
RK21201 |
Abclonal |
50mL |
EUR 109 |
 ViPrimePLUS Taq qPCR Green Master Mix I with ROX, 150rxns |
|
QLMM12-R |
Vivantis |
each |
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 ViPrimePLUS AtTaq qPCR Green Master Mix I with ROX, 150rxns |
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QLMM02-R |
Vivantis |
each |
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) ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns |
|
THK0251 |
Westburg |
each |
EUR 232.17 |
 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns |
|
THK0252 |
Westburg |
each |
EUR 1046.4 |
 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns |
|
THK0253 |
Westburg |
each |
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 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17271 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17272 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17272-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17273 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17273-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17274 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17274-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17275 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17275-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
) BioEasy Master Mix (SYBR Green, No ROX) |
|
TRI-B25L1D |
TRI Biotech |
200T |
EUR 403.2 |
 ViPrimePLUS Taq qPCR Green Master Mix I with Low ROX, 150rxns |
|
QLMM12-LR |
Vivantis |
each |
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) BioEasy Master Mix (SYBR Green, low ROX) |
|
TRI-B25L1C |
TRI Biotech |
200T |
EUR 403.2 |
) BioEasy Master Mix (SYBR Green, high ROX) |
|
TRI-B25L1B |
TRI Biotech |
200T |
EUR 403.2 |
 ViPrimePLUS AtTaq qPCR Green Master Mix I with Low ROX, 150rxns |
|
QLMM02-LR |
Vivantis |
each |
Ask for price |
) ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 ViPrimePLUS Taq qPCR Green Master Mix II with ROX, 150rxns |
|
QLMM17-R |
Vivantis |
each |
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 ViPrimePLUS AtTaq qPCR Green Master Mix II with ROX, 150rxns |
|
QLMM07-R |
Vivantis |
each |
Ask for price |
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17276 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17276-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17277 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17277-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17278 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17278-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17279 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17279-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17280 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17280-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17281 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17281-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 2X SYBR Green Fast qPCR Mix No Rox |
|
RK21200 |
Abclonal |
50mL |
EUR 109 |
 ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns |
|
QLMM17-LR |
Vivantis |
each |
Ask for price |
 ViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX, 150rxns |
|
QLMM07-LR |
Vivantis |
each |
Ask for price |
 2X SYBR Green Fast qPCR Mix with Low ROXII |
|
RK21202 |
Abclonal |
5mL |
EUR 1000.08 |
 Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns |
|
THK0241 |
Westburg |
each |
Ask for price |
 Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns |
|
THK0242 |
Westburg |
each |
Ask for price |
 Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns |
|
THK0243 |
Westburg |
each |
Ask for price |
 HotStart™ 2X Green qPCR Master Mix |
|
K1070-25 |
ApexBio |
25ml |
EUR 392 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
HotStart™ 2X Green qPCR Master Mix |
|
K1070-5 |
ApexBio |
5ml |
EUR 124 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
HotStart™ 2X Green qPCR Master Mix |
|
K1070-50 |
ApexBio |
50ml |
EUR 768 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
) Genious 2X SYBR Green Fast qPCR Mix (No ROX) |
|
RK21205 |
Abclonal |
1 ml |
EUR 324.28 |
|
|
) ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
) ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
 rEVAlution 2X qPCR Master Mix with separate ROX |
|
MBS308019-1000Reactions |
MyBiosource |
1000Reactions |
EUR 650 |
rEVAlution 2X qPCR Master Mix with separate ROX |
|
MBS308019-100Reactions |
MyBiosource |
100Reactions |
EUR 175 |
rEVAlution 2X qPCR Master Mix with separate ROX |
|
MBS308019-500Reactions |
MyBiosource |
500Reactions |
EUR 385 |
 2X qPCR Probe Master Mix with Separate ROX |
|
MBS308018-1000Reactions |
MyBiosource |
1000Reactions |
EUR 1150 |
2X qPCR Probe Master Mix with Separate ROX |
|
MBS308018-100Reactions |
MyBiosource |
100Reactions |
EUR 210 |
2X qPCR Probe Master Mix with Separate ROX |
|
MBS308018-2500Reactions |
MyBiosource |
2500Reactions |
EUR 5350 |
2X qPCR Probe Master Mix with Separate ROX |
|
MBS308018-500Reactions |
MyBiosource |
500Reactions |
EUR 405 |
) 2×SYBR Green qPCR Mix(including ROX) |
|
E37F022-10x5mL |
EnoGene |
10×5mL |
EUR 1360 |
|
Description: Fluorescent quantitative PCR kits |
2×SYBR Green qPCR Mix(including ROX) |
|
E37F022-5mL |
EnoGene |
5mL |
EUR 227 |
|
Description: Fluorescent quantitative PCR kits |
) 2×SYBR Green qPCR Mix (including ROX) |
|
MBS8579841-200Tests |
MyBiosource |
200Tests |
EUR 285 |
2×SYBR Green qPCR Mix (including ROX) |
|
MBS8579841-50Tests |
MyBiosource |
50Tests |
EUR 180 |
2×SYBR Green qPCR Mix (including ROX) |
|
MBS8579841-5x200Tests |
MyBiosource |
5x200Tests |
EUR 1230 |
2×SYBR Green qPCR Mix (including ROX) |
|
MBS8579841-5x50Tests |
MyBiosource |
5x50Tests |
EUR 770 |
) BioEasy Master Mix (SYBR Green) |
|
TRI-B25L1 |
TRI Biotech |
200T |
EUR 403.2 |
 HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-25 |
ApexBio |
25ml |
EUR 424 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-5 |
ApexBio |
5ml |
EUR 132 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-50 |
ApexBio |
50ml |
EUR 826.4 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
) ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
) ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-25 |
ApexBio |
25ml |
EUR 392 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-5 |
ApexBio |
5ml |
EUR 124 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-50 |
ApexBio |
50ml |
EUR 752 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
) Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed) |
|
RK21206 |
Abclonal |
1 ml |
EUR 76.3 |
|
|
 ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with ROX, 150rxns |
|
QLMM14-R |
Vivantis |
each |
Ask for price |
) Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed) |
|
RK21207 |
Abclonal |
1 ml |
EUR 21.26 |
|
|
 ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I with ROX, 150rxns |
|
QLMM04-R |
Vivantis |
each |
Ask for price |
 ABsolute SYBR Green QPCR Mix + ROX 800 rxns |
|
AB-1162/B |
Westburg |
each |
Ask for price |
 ABsolute SYBR Green QPCR Mix + ROX 200 rxns |
|
AB-1163 |
Westburg |
each |
EUR 543.37 |
) SG/ROX qPCR Master Mix (2x) |
|
E0400-01 |
EURx |
100reactions |
EUR 55.59 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
SG/ROX qPCR Master Mix (2x) |
|
E0400-02 |
EURx |
200reactions |
EUR 106.82 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
SG/ROX qPCR Master Mix (2x) |
|
E0400-03 |
EURx |
1000reactions |
EUR 467.61 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
 SYBR Green cRNA PCR Master Mix |
|
SGM002 |
Signosis |
2 ml |
EUR 174 |
) 2 x SYBR Green qPCR Mix(Antibody，ROX) |
|
4302A |
Unibiotest |
1 mL |
EUR 80 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|
4302B |
Unibiotest |
5*1 mL |
EUR 250 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|
4302C |
Unibiotest |
50*1 mL |
EUR 1400 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|
4302D |
Unibiotest |
100*1 mL |
EUR 1600 |
 EnoGene Green qPCR Master Mix |
|
E31M001 |
EnoGene |
500 reaction |
EUR 75 |
 ChamQ SYBR qPCR Master Mix |
|
Q311-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
ChamQ SYBR qPCR Master Mix |
|
Q311-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ SYBR qPCR Master Mix |
|
Q311-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix |
|
Q311-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix |
|
Q311-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
 HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-25 |
ApexBio |
25ml |
EUR 424 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-5 |
ApexBio |
5ml |
EUR 132 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-50 |
ApexBio |
50ml |
EUR 826.4 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
 1ml PrecisionPLUS qPCR Master Mix with ROX |
|
Z-PPLUS-R-1ML |
Novacyt Group |
1ml |
EUR 81 |
|
Description: Precision®PLUS |
 ABsolute QPCR SYBR Green Low Rox Mix 800x50µl |
|
AB-1322/B |
Westburg |
each |
Ask for price |
 ABsolute QPCR SYBR Green Low Rox Mix 200x50µl |
|
AB-1323/A |
Westburg |
each |
EUR 659.45 |
 ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with Low ROX, 150rxns |
|
QLMM14-LR |
Vivantis |
each |
Ask for price |
 ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I with Low ROX, 150rxns |
|
QLMM04-LR |
Vivantis |
each |
Ask for price |
 Apex qPCR GREEN Master Mix |
|
42-116PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
|
Description: 2X, Without ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-117PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
|
Description: 2X, Without ROX, 50ml |
Apex qPCR GREEN Master Mix |
|
42-118PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 338.88 |
|
Description: 2X, Low ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-119PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
|
Description: 2X, Low ROX, 50ml |
Apex qPCR GREEN Master Mix |
|
42-120PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
|
Description: 2X, High ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-121PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 3095.57 |
|
Description: 2X, High ROX, 50ml |
) Fast SG/ROX qPCR Master Mix (2x) |
|
E0413-01 |
EURx |
100reactions |
EUR 61.04 |
|
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
Fast SG/ROX qPCR Master Mix (2x) |
|
E0413-02 |
EURx |
200reactions |
EUR 116.63 |
|
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
Fast SG/ROX qPCR Master Mix (2x) |
|
E0413-03 |
EURx |
1000reactions |
EUR 534.1 |
|
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
) SG/ROX onTaq qPCR Master Mix (2x) |
|
E0407-01 |
EURx |
100reactions |
EUR 54.5 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye |
SG/ROX onTaq qPCR Master Mix (2x) |
|
E0407-02 |
EURx |
200reactions |
EUR 104.64 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye |
SG/ROX onTaq qPCR Master Mix (2x) |
|
E0407-03 |
EURx |
1000reactions |
EUR 455.62 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye |
 ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with ROX, 150rxns |
|
QLMM18-R |
Vivantis |
each |
Ask for price |
 ViPrimePLUS Taq qPCR Master Mix with ROX, 150rxns |
|
QLMM11-R |
Vivantis |
each |
Ask for price |
 ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix II with ROX, 150rxns |
|
QLMM08-R |
Vivantis |
each |
Ask for price |
 ViPrimePLUS AtTaq qPCR Master Mix with ROX, 150rxns |
|
QLMM01-R |
Vivantis |
each |
Ask for price |
, 100rxns) ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns |
|
QLMM10 |
Vivantis |
each |
Ask for price |
 1ml Precision FAST qPCR Master Mix with ROX |
|
Z-PFAST-R-1ML |
Novacyt Group |
1ml |
EUR 90 |
|
Description: Precision®FAST |
 2ml Precision FAST qPCR Master Mix with ROX |
|
Z-PFAST-R-2ML |
Novacyt Group |
2ml |
EUR 161 |
|
Description: Precision®FAST |
 Genious 2X SYBR Green Fast qPCR Mix |
|
RK21204 |
Abclonal |
1 mL |
EUR 76.3 |
|
|
 ChamQ SYBR Color qPCR Master Mix |
|
Q411-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 2X Universal SYBR Green Fast qPCR Mix |
|
MBS9141672-25mL |
MyBiosource |
25mL |
EUR 755 |
2X Universal SYBR Green Fast qPCR Mix |
|
MBS9141672-5mL |
MyBiosource |
5mL |
EUR 255 |
 2X Universal SYBR Green Fast qPCR Mix |
|
RK21203 |
Abclonal |
1 mL |
EUR 21.26 |
|
|
 AceQ Universal SYBR qPCR Master Mix |
|
Q511-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 152.72 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-02-500rxns |
Vazyme |
500 rxns |
EUR 158.14 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 688.5 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 712.94 |
 ChamQ Universal SYBR qPCR Master Mix |
|
Q711-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 152.72 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-02-500rxns |
Vazyme |
500 rxns |
EUR 158.14 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 688.5 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 712.94 |
 miRNA Universal SYBR qPCR Master Mix |
|
MQ101-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 38.9 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-01-125rxns |
Vazyme |
125 rxns |
EUR 40.28 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 145 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-02-500rxns |
Vazyme |
500 rxns |
EUR 150.15 |
) ABsolute Blue QPCR SYBR Green ROX Mix (16x 1.25ml) |
|
AB-4162/B |
Westburg |
each |
Ask for price |
 BrightCycle Universal SYBR Green qPCR Mix with UDG |
|
RK21219 |
Abclonal |
5mL |
EUR 29.98 |
 iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul |
|
A4183-4x125ML500Rxns |
Biomatik Corporation |
4x1.25ML (500Rxns) |
EUR 200.2 |
|
Description: Biotechnology |
 ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns |
|
QLMM12 |
Vivantis |
each |
Ask for price |
 ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns |
|
QLMM02 |
Vivantis |
each |
Ask for price |
 ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with Low ROX, 150rxns |
|
QLMM18-LR |
Vivantis |
each |
Ask for price |
 lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|
M-PCR-173L |
MiTeGen |
960 reactions x 20 µl |
EUR 897 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|
M-PCR-173S |
MiTeGen |
192 reactions x 20 µl |
EUR 224 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
, plus ROX Solution) SG qPCR Master Mix (2x), plus ROX Solution |
|
E0402-01 |
EURx |
100reactions |
EUR 55.59 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye |
SG qPCR Master Mix (2x), plus ROX Solution |
|
E0402-02 |
EURx |
200reactions |
EUR 106.82 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye |
SG qPCR Master Mix (2x), plus ROX Solution |
|
E0402-03 |
EURx |
1000reactions |
EUR 467.61 |
|
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye |
, plus ROX Solution) MP qPCR Master Mix (2x), plus ROX Solution |
|
E0425-01 |
EURx |
100reactions |
EUR 58.86 |
|
Description: For quantitative multiplex real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye |
PANDAA-quantified DRMs current at frequency ≥5% (2 h from nucleic acid to consequence) with a sensitivity and specificity of 96.9% and 97.5%, respectively. PANDAA is an revolutionary development with applicability to any pathogen the place target-proximal genetic variability hinders diagnostic growth.
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