Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

An correct genetic diagnostic is essential for enough affected person administration and the suitability of healthcare techniques. The scientific problem lies in growing strategies to discriminate these sufferers with sure genetic variations current in tumor cells at low concentrations. We report a technique referred to as enhanced uneven blocked qPCR (EAB-qPCR) that promotes the blocker annealing towards the primer-template hybrid controlling thermal biking and response circumstances with nonmodified oligonucleotides. Actual-time fluorescent amplification curves of wild-type alleles had been delayed by about eight cycles for EAB-qPCR, in comparison with standard blocked qPCR approaches.

This methodology diminished the amplification of native DNA variants (blocking proportion 99.7%) and enabled the efficient enrichment of low-level DNA mutations. Wonderful efficiency was estimated for the detection of mutated alleles in sensitivity (as much as 0.5% mutant/whole DNA) and reproducibility phrases, with a relative commonplace deviation under 2.8%. The strategy was efficiently utilized to the mutational evaluation of metastatic colorectal carcinoma from biopsied tissues.

The decided single-nucleotide mutations within the KRAS oncogene (codon 12-13) completely agreed with these obtained from next-generation sequencing. EAB-qPCR is an correct low cost methodology and might be simply included into day by day routine to detect mutant alleles. Therefore, these options are particularly attention-grabbing to facilitate the analysis and prognosis of a number of scientific ailments.

Quantification of Actaea racemosa L. (black cohosh) from a few of its potential adulterants utilizing qPCR and dPCR strategies

The demand for well-liked pure well being merchandise (NHPs) similar to Black Cohosh is growing significantly, which in flip challenges high quality assurance (QA) all through the availability chain. To detect and quantify the goal species current in a given NHP, DNA-based molecular strategies similar to Actual-time quantitative PCR (qPCR) and digital PCR (dPCR) are commonplace instruments within the meals and pathogen testing industries.
There’s a hole within the literature regarding validated quantitative PCR strategies for botanicals that may be utilized for QA and good manufacturing practices. The target of this research is to develop an environment friendly quantification methodology utilizing qPCR and dPCR strategies for the detection and quantification of Actaea racemosa (Black cohosh) NHPs from its potential adulterants. These developed strategies are validated for applicability on business NHPs.
Species-specific hydrolysis probe assays had been designed to research the black cohosh NHPs utilizing qPCR and dPCR strategies. The outcomes confirmed that the developed qPCR and dPCR strategies are extremely exact for figuring out and quantifying black cohosh NHPs, indicating their potential applicability in future routine industrial and laboratory testing. This permits a single qPCR check to find out not solely the presence of a particular botanical, but additionally the quantity when combined with an adulterant.

Quantification of Legionella pneumophila by qPCR and tradition in faucet water with completely different concentrations of residual disinfectants and heterotrophic micro organism

Legionellosis prevalence is growing in the USA. This illness is precipitated primarily by the bacterium Legionella pneumophila present in water and transmitted by aerosol inhalation. This pathogen has a gradual progress fee and may “disguise” in amoeba, making it tough to watch by the normal tradition methodology on selective media. Faucet water samples (n = 358) collected throughout the USA had been examined for L. pneumophila by each tradition and quantitative Polymerase Chain Response (qPCR).
The presence of different micro organism was quantified by heterotrophic plate counts (HPC). Residual disinfectant concentrations (free chlorine or monochloramine) had been measured in all samples. Legionella pneumophila had the best prevalence and focus within the chlorinated water samples that had a free‑chlorine worth of lower than 0.2 mg Cl2/L. In whole, 24% (87/358) of the samples had been constructive for L. pneumophila both by qPCR or 3% (11/358) had been constructive by tradition.
Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene
In chloramine-treated samples, L. pneumophila was detected by qPCR in 21% (31/148) and 1% (2/148) by tradition, regardless of a excessive monochloramine residual >1 mg Cl2/L. Regardless of the presence of a excessive disinfectant residual (>1 mg Cl2/L), HPC counts had been substantial. This research signifies that each tradition and qPCR strategies have limitations when predicting a possible danger for illness related to L. pneumophila in faucet water. Measuring disinfectant residuals and quantifying HPC in water samples could also be helpful adjunct parameters for lowering Legionellosis’ danger from public water provides at high-risk areas.

PANDAA deliberately violates standard qPCR design to allow sturdy, mismatch-agnostic detection of extremely polymorphic pathogens

Delicate and reproducible diagnostics are elementary to containing the unfold of present and rising pathogens. Regardless of the reliance of scientific virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding areas produces false-negative outcomes.
We systematically violated canonical qPCR design rules to develop a Pan-Degenerate Amplification and Adaptation (PANDAA), a degree mutation assay that mitigates the influence of sequence variation on probe-based qPCR efficiency. Utilizing HIV-1 as a mannequin system, we optimized and validated PANDAA to detect HIV drug resistance mutations (DRMs). Extremely-degenerate primers with 3′ termini overlapping the probe-binding web site adapt the goal by site-directed mutagenesis throughout qPCR to exchange DRM-proximal sequence variation.

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Description: SYBR Green qPCR Master Mix (No ROX)

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Description: SYBR Green qPCR Master Mix (No ROX)

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Description: SYBR Green qPCR Master Mix (No ROX)

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Description: SYBR Green qPCR Master Mix (No ROX)

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Description: SYBR Green qPCR Master Mix (No ROX)

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HY-K0523 5 mL (500 rxns )
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Description: SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 135.6

2x SYBR Green qPCR Master Mix

B21202 5 mL
EUR 268.8
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

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B21203 25 mL
EUR 1027.2
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ig SYBR Green qPCR 2X Master Mix

3354 200
EUR 92
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

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Q111-02 500 rxn (20 μl/rxn)
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Q321-02 500 rxns (20 μl/rxn)
EUR 120.55

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Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns

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EUR 232.17

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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

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17271-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

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17272 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

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17272-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

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EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

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EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

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17274 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17274-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17275 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17275-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

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TRI-B25L1C 200T
EUR 403.2

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Q421-02 500 rxns (20 μl/rxn)
EUR 140.63

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EUR 145.62

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EUR 634

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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

2X SYBR Green Fast qPCR Mix No Rox

RK21200 50mL
EUR 109

ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns

QLMM17-LR each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX, 150rxns

QLMM07-LR each Ask for price

2X SYBR Green Fast qPCR Mix with Low ROXII

RK21202 5mL
EUR 1000.08

Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns

THK0241 each Ask for price

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Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns

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HotStart™ 2X Green qPCR Master Mix

K1070-25 25ml
EUR 392
Description: Reverse Transcription & PCR|qPCR

HotStart™ 2X Green qPCR Master Mix

K1070-5 5ml
EUR 124
Description: Reverse Transcription & PCR|qPCR

HotStart™ 2X Green qPCR Master Mix

K1070-50 50ml
EUR 768
Description: Reverse Transcription & PCR|qPCR

Genious 2X SYBR Green Fast qPCR Mix (No ROX)

RK21205 1 ml
EUR 324.28

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02 500 rxns (20 μl/rxn)
EUR 120.55

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Q331-02-500rxns 500 rxns
EUR 124.83

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Q331-03 2,500 rxns (20 μl/rxn)
EUR 543.5

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EUR 562.79

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Q341-02 500 rxns (20 μl/rxn)
EUR 120.55

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EUR 124.83

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Q341-03-2500rxns 2,500 rxns
EUR 562.79

rEVAlution 2X qPCR Master Mix with separate ROX

MBS308019-1000Reactions 1000Reactions
EUR 650

rEVAlution 2X qPCR Master Mix with separate ROX

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EUR 175

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EUR 385

2X qPCR Probe Master Mix with Separate ROX

MBS308018-1000Reactions 1000Reactions
EUR 1150

2X qPCR Probe Master Mix with Separate ROX

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EUR 210

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MBS308018-2500Reactions 2500Reactions
EUR 5350

2X qPCR Probe Master Mix with Separate ROX

MBS308018-500Reactions 500Reactions
EUR 405

BioEasy Master Mix (SYBR Green)

TRI-B25L1 200T
EUR 403.2

2×SYBR Green qPCR Mix(including ROX)

E37F022-10x5mL 10×5mL
EUR 1360
Description: Fluorescent quantitative PCR kits

2×SYBR Green qPCR Mix(including ROX)

E37F022-5mL 5mL
EUR 227
Description: Fluorescent quantitative PCR kits

2×SYBR Green qPCR Mix (including ROX)

MBS8579841-200Tests 200Tests
EUR 285

2×SYBR Green qPCR Mix (including ROX)

MBS8579841-50Tests 50Tests
EUR 180

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EUR 1230

2×SYBR Green qPCR Mix (including ROX)

MBS8579841-5x50Tests 5x50Tests
EUR 770

HotStart™ 2X FAST Green qPCR Master Mix

K1171-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02-500rxns 500 rxns
EUR 145.62

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EUR 634

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EUR 656.51

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EUR 140.63

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EUR 145.62

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EUR 634

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-03-2500rxns 2,500 rxns
EUR 656.51

HotStart™ Universal 2X Green qPCR Master Mix

K1170-25 25ml
EUR 392
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X Green qPCR Master Mix

K1170-5 5ml
EUR 124
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X Green qPCR Master Mix

K1170-50 50ml
EUR 752
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed)

RK21206 1 ml
EUR 76.3

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with ROX, 150rxns

QLMM14-R each Ask for price

Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed)

RK21207 1 ml
EUR 21.26

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ABsolute SYBR Green QPCR Mix + ROX 800 rxns

AB-1162/B each Ask for price

ABsolute SYBR Green QPCR Mix + ROX 200 rxns

AB-1163 each
EUR 543.37

SG/ROX qPCR Master Mix (2x)

E0400-01 100reactions
EUR 55.59
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

SG/ROX qPCR Master Mix (2x)

E0400-02 200reactions
EUR 106.82
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

SG/ROX qPCR Master Mix (2x)

E0400-03 1000reactions
EUR 467.61
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

SYBR Green cRNA PCR Master Mix

SGM002 2 ml
EUR 174

2 x SYBR Green qPCR Mix(Antibody,ROX)

4302A 1 mL
EUR 80

2 x SYBR Green qPCR Mix(Antibody,ROX)

4302B 5*1 mL
EUR 250

2 x SYBR Green qPCR Mix(Antibody,ROX)

4302C 50*1 mL
EUR 1400

2 x SYBR Green qPCR Mix(Antibody,ROX)

4302D 100*1 mL
EUR 1600

EnoGene Green qPCR Master Mix

E31M001 500 reaction
EUR 75

ChamQ SYBR qPCR Master Mix

Q311-00-100rxns 100 rxns
EUR 1.09

ChamQ SYBR qPCR Master Mix

Q311-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix

Q311-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix

Q311-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix

Q311-03-2500rxns 2,500 rxns
EUR 562.79

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

1ml PrecisionPLUS qPCR Master Mix with ROX

Z-PPLUS-R-1ML 1ml
EUR 81
Description: Precision®PLUS

ABsolute QPCR SYBR Green Low Rox Mix 800x50µl

AB-1322/B each Ask for price

ABsolute QPCR SYBR Green Low Rox Mix 200x50µl

AB-1323/A each
EUR 659.45

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with Low ROX, 150rxns

QLMM14-LR each Ask for price

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I with Low ROX, 150rxns

QLMM04-LR each Ask for price

Apex qPCR GREEN Master Mix

42-116PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix

42-117PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix

42-118PG 400 x 25µl Reactions/Unit
EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix

42-119PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix

42-120PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix

42-121PG 4000 x 25µl Reactions/Unit
EUR 3095.57
Description: 2X, High ROX, 50ml

Fast SG/ROX qPCR Master Mix (2x)

E0413-01 100reactions
EUR 61.04
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

Fast SG/ROX qPCR Master Mix (2x)

E0413-02 200reactions
EUR 116.63
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

Fast SG/ROX qPCR Master Mix (2x)

E0413-03 1000reactions
EUR 534.1
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

SG/ROX onTaq qPCR Master Mix (2x)

E0407-01 100reactions
EUR 54.5
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye

SG/ROX onTaq qPCR Master Mix (2x)

E0407-02 200reactions
EUR 104.64
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye

SG/ROX onTaq qPCR Master Mix (2x)

E0407-03 1000reactions
EUR 455.62
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with ROX, 150rxns

QLMM18-R each Ask for price

ViPrimePLUS Taq qPCR Master Mix with ROX, 150rxns

QLMM11-R each Ask for price

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix II with ROX, 150rxns

QLMM08-R each Ask for price

ViPrimePLUS AtTaq qPCR Master Mix with ROX, 150rxns

QLMM01-R each Ask for price

ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns

QLMM10 each Ask for price

1ml Precision FAST qPCR Master Mix with ROX

Z-PFAST-R-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix with ROX

Z-PFAST-R-2ML 2ml
EUR 161
Description: Precision®FAST

Genious 2X SYBR Green Fast qPCR Mix

RK21204 1 mL
EUR 76.3

ChamQ SYBR Color qPCR Master Mix

Q411-00-100rxns 100 rxns
EUR 1.09

ChamQ SYBR Color qPCR Master Mix

Q411-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix

Q411-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix

Q411-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix

Q411-03-2500rxns 2,500 rxns
EUR 656.51

2X Universal SYBR Green Fast qPCR Mix  

MBS9141672-25mL 25mL
EUR 755

2X Universal SYBR Green Fast qPCR Mix  

MBS9141672-5mL 5mL
EUR 255

2X Universal SYBR Green Fast qPCR Mix

RK21203 1 mL
EUR 21.26

AceQ Universal SYBR qPCR Master Mix

Q511-00-100rxns 100 rxns
EUR 1.09

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 152.72

AceQ Universal SYBR qPCR Master Mix

Q511-02-500rxns 500 rxns
EUR 158.14

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 688.5

AceQ Universal SYBR qPCR Master Mix

Q511-03-2500rxns 2,500 rxns
EUR 712.94

miRNA Universal SYBR qPCR Master Mix

MQ101-00-100rxns 100 rxns
EUR 1.09

miRNA Universal SYBR qPCR Master Mix

MQ101-01 125 rxn(20 μl/rxn)
EUR 38.9

miRNA Universal SYBR qPCR Master Mix

MQ101-01-125rxns 125 rxns
EUR 40.28

miRNA Universal SYBR qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 145

miRNA Universal SYBR qPCR Master Mix

MQ101-02-500rxns 500 rxns
EUR 150.15

ChamQ Universal SYBR qPCR Master Mix

Q711-00-100rxns 100 rxns
EUR 1.09

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 152.72

ChamQ Universal SYBR qPCR Master Mix

Q711-02-500rxns 500 rxns
EUR 158.14

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 688.5

ChamQ Universal SYBR qPCR Master Mix

Q711-03-2500rxns 2,500 rxns
EUR 712.94

ABsolute Blue QPCR SYBR Green ROX Mix (16x 1.25ml)

AB-4162/B each Ask for price

BrightCycle Universal SYBR Green qPCR Mix with UDG

RK21219 5mL
EUR 29.98

iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul

A4183-4x125ML500Rxns 4x1.25ML (500Rxns)
EUR 200.2
Description: Biotechnology

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns

QLMM12 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns

QLMM02 each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with Low ROX, 150rxns

QLMM18-LR each Ask for price

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173L 960 reactions x 20 µl
EUR 897
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173S 192 reactions x 20 µl
EUR 224
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

SG qPCR Master Mix (2x), plus ROX Solution

E0402-01 100reactions
EUR 55.59
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

SG qPCR Master Mix (2x), plus ROX Solution

E0402-02 200reactions
EUR 106.82
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

SG qPCR Master Mix (2x), plus ROX Solution

E0402-03 1000reactions
EUR 467.61
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

MP qPCR Master Mix (2x), plus ROX Solution

E0425-01 100reactions
EUR 58.86
Description: For quantitative multiplex real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye
PANDAA-quantified DRMs current at frequency ≥5% (2 h from nucleic acid to consequence) with a sensitivity and specificity of 96.9% and 97.5%, respectively. PANDAA is an revolutionary development with applicability to any pathogen the place target-proximal genetic variability hinders diagnostic growth.

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