Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene

An correct genetic diagnostic is essential for enough affected person administration and the suitability of healthcare techniques. The scientific problem lies in growing strategies to discriminate these sufferers with sure genetic variations current in tumor cells at low concentrations. We report a technique referred to as enhanced uneven blocked qPCR (EAB-qPCR) that promotes the blocker annealing towards the primer-template hybrid controlling thermal biking and response circumstances with nonmodified oligonucleotides. Actual-time fluorescent amplification curves of wild-type alleles had been delayed by about eight cycles for EAB-qPCR, in comparison with standard blocked qPCR approaches.

This methodology diminished the amplification of native DNA variants (blocking proportion 99.7%) and enabled the efficient enrichment of low-level DNA mutations. Wonderful efficiency was estimated for the detection of mutated alleles in sensitivity (as much as 0.5% mutant/whole DNA) and reproducibility phrases, with a relative commonplace deviation under 2.8%. The strategy was efficiently utilized to the mutational evaluation of metastatic colorectal carcinoma from biopsied tissues.

The decided single-nucleotide mutations within the KRAS oncogene (codon 12-13) completely agreed with these obtained from next-generation sequencing. EAB-qPCR is an correct low cost methodology and might be simply included into day by day routine to detect mutant alleles. Therefore, these options are particularly attention-grabbing to facilitate the analysis and prognosis of a number of scientific ailments.

Quantification of Actaea racemosa L. (black cohosh) from a few of its potential adulterants utilizing qPCR and dPCR strategies

The demand for well-liked pure well being merchandise (NHPs) similar to Black Cohosh is growing significantly, which in flip challenges high quality assurance (QA) all through the availability chain. To detect and quantify the goal species current in a given NHP, DNA-based molecular strategies similar to Actual-time quantitative PCR (qPCR) and digital PCR (dPCR) are commonplace instruments within the meals and pathogen testing industries.
There’s a hole within the literature regarding validated quantitative PCR strategies for botanicals that may be utilized for QA and good manufacturing practices. The target of this research is to develop an environment friendly quantification methodology utilizing qPCR and dPCR strategies for the detection and quantification of Actaea racemosa (Black cohosh) NHPs from its potential adulterants. These developed strategies are validated for applicability on business NHPs.
Species-specific hydrolysis probe assays had been designed to research the black cohosh NHPs utilizing qPCR and dPCR strategies. The outcomes confirmed that the developed qPCR and dPCR strategies are extremely exact for figuring out and quantifying black cohosh NHPs, indicating their potential applicability in future routine industrial and laboratory testing. This permits a single qPCR check to find out not solely the presence of a particular botanical, but additionally the quantity when combined with an adulterant.

Quantification of Legionella pneumophila by qPCR and tradition in faucet water with completely different concentrations of residual disinfectants and heterotrophic micro organism

Legionellosis prevalence is growing in the USA. This illness is precipitated primarily by the bacterium Legionella pneumophila present in water and transmitted by aerosol inhalation. This pathogen has a gradual progress fee and may “disguise” in amoeba, making it tough to watch by the normal tradition methodology on selective media. Faucet water samples (n = 358) collected throughout the USA had been examined for L. pneumophila by each tradition and quantitative Polymerase Chain Response (qPCR).
The presence of different micro organism was quantified by heterotrophic plate counts (HPC). Residual disinfectant concentrations (free chlorine or monochloramine) had been measured in all samples. Legionella pneumophila had the best prevalence and focus within the chlorinated water samples that had a free‑chlorine worth of lower than 0.2 mg Cl2/L. In whole, 24% (87/358) of the samples had been constructive for L. pneumophila both by qPCR or 3% (11/358) had been constructive by tradition.
Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene
In chloramine-treated samples, L. pneumophila was detected by qPCR in 21% (31/148) and 1% (2/148) by tradition, regardless of a excessive monochloramine residual >1 mg Cl2/L. Regardless of the presence of a excessive disinfectant residual (>1 mg Cl2/L), HPC counts had been substantial. This research signifies that each tradition and qPCR strategies have limitations when predicting a possible danger for illness related to L. pneumophila in faucet water. Measuring disinfectant residuals and quantifying HPC in water samples could also be helpful adjunct parameters for lowering Legionellosis’ danger from public water provides at high-risk areas.

PANDAA deliberately violates standard qPCR design to allow sturdy, mismatch-agnostic detection of extremely polymorphic pathogens

Delicate and reproducible diagnostics are elementary to containing the unfold of present and rising pathogens. Regardless of the reliance of scientific virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding areas produces false-negative outcomes.
We systematically violated canonical qPCR design rules to develop a Pan-Degenerate Amplification and Adaptation (PANDAA), a degree mutation assay that mitigates the influence of sequence variation on probe-based qPCR efficiency. Utilizing HIV-1 as a mannequin system, we optimized and validated PANDAA to detect HIV drug resistance mutations (DRMs). Extremely-degenerate primers with 3′ termini overlapping the probe-binding web site adapt the goal by site-directed mutagenesis throughout qPCR to exchange DRM-proximal sequence variation.

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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17273-5mL 5 mL
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17275-5mL 5 mL
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17278-1mL 1 mL
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*
17270 1 mL
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17272 1 mL
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TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*
17273 5 mL
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17274 1 mL
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TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*
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17277 5 mL
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17278 1 mL
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
17279 5 mL
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17280 1 mL
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17281 5 mL
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Q431-02 500 rxns (20 μl/rxn)
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Q511-03 2500 rxn (20 μl/rxn)
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Q711-02 500 rxn (20 μl/rxn)
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Q711-03 2500 rxn (20 μl/rxn)
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RK21204 1 mL Ask for price

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RK21203 1 mL Ask for price

iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul
A4183-4x125ML500Rxns 4x1.25ML (500Rxns)
EUR 200.2
Description: Biotechnology

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RK21219 5mL
EUR 475

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Q712-02 500 rxns (20 μl/rxn)
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Q712-03 2,500 rxns (20 μl/rxn)
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MQ101-01 125 rxn(20 μl/rxn)
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MQ101-02 500 rxn(20 μl/rxn)
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lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173L 960 reactions x 20 µl
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

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M-PCR-173S 192 reactions x 20 µl
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)
31014-2 50x1mL
EUR 4291.2
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Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)
31015-2 50x1mL
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31014-1 5x1mL
EUR 524.4
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)
31015-1 5x1mL
EUR 524.4
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)
31042-1 5x1mL
EUR 326.4
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31042-20mL 2x10mL
EUR 592.8
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)
31042-T 1mL
EUR 136.8
Description: Minimum order quantity: 1 unit of 1mL

Entrans 2X qPCR Probe Master Mix with UDG
RK21222 50RXN
EUR 71

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)
31014-T 1mL
EUR 160.8
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31015-T 1mL
EUR 160.8
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Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)
31014 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-001 1ml
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P0346-002 2x1ml
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P0346-010 10x1ml
EUR 1632

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)
31015 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

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BS9291 5X1mL, 5ml
EUR 251.4

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BS9292 1ml
EUR 115.68

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Q5600-001 1ml
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amfiSure qProbe Q-PCR Master Mix(2X), High Rox
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EUR 400.8

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QPCR002-L 4X1.25ml
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RK21208 40 RXN Ask for price

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W141 200 rxn
EUR 199

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*
17282-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*
17283-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*
17284-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*
17285-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

EZGreen 2x qPCR Master Mix, 40Rxns-50ul
A4157-1ML40Rxns 1ML (40Rxns)
EUR 55
Description: Biotechnology

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX*
17286-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX*
17287-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions 100 Reactions
EUR 392.4

Fast Probe Master Mix with Rox (5000 rxn)
31016-2 50x1mL
EUR 4645.2
Description: Minimum order quantity: 1 unit of 50x1mL

Bio-Star Mastermix with SYBR (2X) Low-ROX
Y250 100 rcs (2,5 ml)
EUR 122.4

1-Step 2X RT-PCR Master Mix-SYBR Green-HP (High Processibility)
W142 200 rxn
EUR 199

Entrans 2X qPCR Probe Master Mix V2
RK21212 50RXN
EUR 67.5

Bio-Star Mastermix with SYBR (2X) High-ROX
Y240 100 rcs (2,5 ml)
EUR 122.4

Fast Probe Master Mix with Rox (200 rxn)
31016 2x1mL
EUR 280.8
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Probe Master Mix with Rox (500 rxn)
31016-1 5x1mL
EUR 559.2
Description: Minimum order quantity: 1 unit of 5x1mL

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*
17282 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*
17283 5 mL
EUR 308

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*
17284 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*
17285 5 mL
EUR 308

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*
17286 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*
17287 5 mL
EUR 308

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17288-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17289-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17290-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17291-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17292-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17293-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn)
QPCR001-R 4X1.25ml
EUR 303.25

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17288 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17289 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17290 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17291 5 mL
EUR 308

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)
9-31045
  • EUR 136.80
  • EUR 578.40
  • EUR 319.20
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17292 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17293 5 mL
EUR 308

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)
9-31046
  • EUR 136.80
  • EUR 578.40
  • EUR 319.20
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

RT Master Mix for qPCR
HY-K0510 1 mL (100 rxns)
EUR 349.2

TaqProbe 2X qPCR MasterMix-Low ROX
MasterMix-PL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials
S210 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials
S210L 500 rcs (12,5 ml)
EUR 627.6

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

AceQ qPCR Probe Master Mix
Q112-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR Probe Master Mix
Q112-03 2500 rxn (20 μl/rxn)
EUR 775.2

Fast Probe Master Mix with Rox, trial size (100 rxn)
31016-T 1mL
EUR 169.2
Description: Minimum order quantity: 1 unit of 1mL

ABScript III RT Master Mix for qPCR with gDNA Remover
RK20429 10RXN
EUR 20

ABScript Neo RT Master Mix for qPCR with gDNA Remover
RK20433 100RXN
EUR 30

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 451.2

qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S220 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S220L 500 rcs (12,5 ml)
EUR 734.4

qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials
S225 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials
S225L 500 rcs (12,5 ml)
EUR 734.4

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
S170 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
S170L 500 rcs (12,5 ml)
EUR 627.6

Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX)
RK21206 1 ml Ask for price

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S175 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S175L 500 rcs (12,5 ml)
EUR 627.6

Bio-Star Mastermix with SYBR (2X) BLUE
Y220 100 rcs (2,5 ml)
EUR 112.8

Gloria Nova HS 2X Master Mix with Dye
RK20728 1mL
EUR 107.5

qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S215 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S215L 500 rcs (12,5 ml)
EUR 627.6

TaqProbe 2X qPCR MasterMix-ROX
MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (500 reactions)
31044-1 1kit
EUR 396
Description: Minimum order quantity: 1 unit of 1kit

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (100 reactions)
31044-T 1kit
EUR 136.8
Description: Minimum order quantity: 1 unit of 1kit

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 451.2

qPCR Master Mix E4 (SafeLine: EvaGreen, dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S180 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix E4 (SafeLine: EvaGreen, dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S180L 500 rcs (12,5 ml)
EUR 734.4

qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials
S185 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials
S185L 500 rcs (12,5 ml)
EUR 734.4

BrightGreen Express 2X qPCR MasterMix-Low ROX
MasterMix-EL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)
31020-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Bio-Star Mastermix with SYBR (2X) no dye
Y210 100 rcs (2,5 ml)
EUR 106.8

Accuris qMax Green, No Rox qPCR Mix, 1000 reactions
PR2000-N-1000 1 PC
EUR 623.8

Accuris qMax Green, Low Rox qPCR Mix, 1000 reactions
PR2000-L-1000 1 PC
EUR 623.8

Fast Plus EvaGreen qPCR Master Mix (500 rxn)
31020-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Taqman Master Mix-Low ROX
M1123-500 each
EUR 529.2

Accuris qMax Green, No Rox qPCR Mix, 100 reactions
PR2000-N-100 1 PC
EUR 77.7

Accuris qMax Green, No Rox qPCR Mix, 500 reactions
PR2000-N-500 1 PC
EUR 340.2

Accuris qMax Green, High Rox qPCR Mix, 1000 reactions
PR2000-H-1000 1 PC
EUR 623.8
PANDAA-quantified DRMs current at frequency ≥5% (2 h from nucleic acid to consequence) with a sensitivity and specificity of 96.9% and 97.5%, respectively. PANDAA is an revolutionary development with applicability to any pathogen the place target-proximal genetic variability hinders diagnostic growth.
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