Analysis of the cox1 gene in Echinococcus granulosus from sheep in northeast Iran using PCR high-resolution melting (qPCR-HRM) curve analysis
Echinococcus granulosus, the etiologic agent of echinococcosis, is among the most essential zoonotic helminthes worldwide. Information of E. granulosus species and genotypes has essential implications for epidemiology, management, and prevention of illnesses in addition to future vaccine and drug designs. There are a lot of molecular strategies developed to outline genotypes of E. granulosus, amongst them excessive decision melting (HRM) evaluation, as a brand new strategy, is a single step and closed tube methodology. It’s acceptable for quick screening of huge variety of isolates. This method is an correct, consumer pleasant, cost-effective, quick and easy methodology, which doesn’t want post-PCR processes.
Between March and lst august 2016, of 726 sheep examined in abattoirs in Razavi Khorasan province, Northeast Iran, 109 harboured cystic echincoccosis lesions (liver samples= 65 and lung samples= 44) which have been collected for evaluation. Complete genomic DNA was extracted from every pattern and amplified for the presence of polymorphism within the mitochondrial cox1 gene of Echinococcus granulosus utilizing a excessive decision melting curve (HRM) methodology.
A complete of 109 hydatid cyst samples analyzed by PCR high-resolution melting (qPCR-HRM) curve of the cox1 gene, all isolates have been recognized as G1 genotype (sheep pressure). G1 is the predominant genotype in sheep in northeast of Iran. The excessive incidence of the G1 genotype (identified to be the predominant E. granulosus genotype infecting people globally) in sheep has appreciable implications for hydatid illness management packages on this space.
Improvement and validation of a multiplex qPCR assay for detection and relative quantification of HPV16 and HPV18 E6 and E7 oncogenes
Human papillomaviruses (HPV) play a key function in selling human anogenital cancers. Present high-risk HPV screening or analysis exams contain cytological or molecular methods largely primarily based on qualitative HPV DNA detection. Right here, we describe the event of a speedy quantitative polymerase chain response (qPCR) detection take a look at of HPV16 and HPV18 oncogenes (E6 and E7) normalized on human gene encoding GAPDH. Optimized qPCR parameters have been outlined, and analytical specificities have been validated. The restrict of detection was 101 for all genes examined.
Assay performances have been evaluated on medical samples (n = 96). Concordance between the Xpert HPV assay and the triplex assay developed right here was 93.44% for HPV16 and 73.58% for HPV18. HPV co-infections have been detected in 15 samples. The programs developed within the current examine can be utilized in complement to conventional HPV exams for particularly validating the presence of HPV16 and/or HPV18. It will also be used for the follow-up of sufferers with confirmed an infection and liable to creating lesions, by means of the quantification of E6 and E7 oncogene expression (mRNA) normalized on the GAPDH expression ranges.
A multiplex TaqMan qPCR assay for detection and quantification of clade 1 and clade 2 isolates of Pseudoperonospora cubensis and Pseudoperonospora humuli
The flexibility to detect and quantify aerially dispersed plant pathogens is crucial for creating efficient illness management measures and epidemiological fashions that optimize the timing for management . There’s an acute want for managing the downy mildew pathogens infecting cucurbits and hop incited by members of the genus Pseudoperonospora (P. cubensis clade 1 and a couple of isolates and P. humuli, respectively).
A extremely particular multiplex TaqMan qPCR assay concentrating on distinctive sequences within the pathogens’ mitochondrial genomes was developed that permits detection of all three taxa in a single multiplexed amplification. An inside management included within the response evaluated if outcomes have been influenced by PCR inhibitors that may make it by means of the DNA extraction course of.
Dependable quantification of inoculum as little as three sporangia in a pattern was noticed. The multiplexed assay was examined with DNA extracted from purified sporangia, contaminated plant tissue and environmental samples collected on impaction spore traps samplers. The flexibility to precisely detect and concurrently quantify all three pathogens in a single multiplexed amplification ought to enhance administration choices for controlling the illnesses they trigger.

Identification and validation of reference genes for dependable evaluation of differential gene expression throughout antibiotic induced persister formation in Klebsiella pneumoniae utilizing qPCR
The examine of differential gene expression in persister cells is compounded by ceasure of typical mobile metabolic pathways throughout persistence. There’s, therefore, a requirement to establish and validate appropriate reference genes whose expression stays secure throughout persistence. We evaluated the suitability of 5 genes viz. dnaJ, groEL, rpoB, kp751, kp4432 as references to check gene expression utilizing real-time polymerase chain response (qPCR) throughout persister cell formation in Klebsiella pneumoniae. Outcomes obtained confirmed that whereas dnaJ and groEL suffered from unstable expression; rpoB, kp751 and kp4432 confirmed secure expression.
Additional, it was noticed that information normalization utilizing both of the secure genes viz. rpoB, kp751, kp4432 alone, resulted in both too low expression ranges or too excessive variation amongst replicates. Our examine signifies the concurrent use of kp4432 and rpoB as reference genes to be probably the most appropriate for dependable evaluation of differential gene expression throughout antibiotic induced persister formation in Okay. pneumoniae. kp4432 and rpoB encode NAD-dependant phenylacetaldehyde dehydrogenase and DNA-directed RNA polymerase beta subunit respectively.
The result of this examine will enhance the utility of qPCR in finding out the temporal adjustments in gene expression throughout persistence. The examine can even help in understanding mechanisms underlying persister cell formation notably in Okay. pneumoniae.
Comparability of qPCR and Metabarcoding Strategies as Instruments for the Detection of Airborne Inoculum of Forest Fungal Pathogens
Forest illnesses brought on by invasive fungal pathogens have gotten extra widespread, typically with dramatic penalties to forest ecosystems. The event of early detection programs is important for environment friendly surveillance and to mitigate the impression of invasive pathogens. Windborne spores are an essential pathway for introduction of fungal pathogens into new areas; the design of spore trapping units tailored to forests, able to gathering various kinds of spores, and aligned with improvement of environment friendly molecular strategies for detection of the pathogen, ought to assist forest managers anticipate new illness outbreaks.
Two varieties of Rotorod samplers have been evaluated for the gathering of airborne inoculum of forest fungal pathogens with a variety of spore sizes in 5 forest varieties. Detection was by particular quantitative PCR (qPCR) and by high-throughput sequencing (HTS) of amplified inside transcribed spacer sequences utilizing a brand new bioinformatic pipeline, FungiSearch, developed for diagnostic functions. Validation of the pipeline was performed on mock communities of 10 fungal species belonging to totally different taxa.
Pro QPCR SuperMix Kit |
K5053400 |
Biochain |
400 reactions |
EUR 291 |
Green qPCR SuperMix |
20-abx098031 |
Abbexa |
-
EUR 1245.60
-
EUR 526.80
-
EUR 760.80
|
|
|
Probe qPCR SuperMix |
20-abx098040 |
Abbexa |
-
EUR 1245.60
-
EUR 526.80
-
EUR 760.80
|
|
|
Pro QPCR SuperMix Kit - ROX premixed |
K5056200 |
Biochain |
200 reactions |
EUR 161 |
Pro QPCR SuperMix Kit - ROX premixed |
K5056400 |
Biochain |
400 reactions |
EUR 291 |
Green qPCR SuperMix UDG |
20-abx098032 |
Abbexa |
-
EUR 1362.00
-
EUR 543.60
-
EUR 794.40
|
|
|
Top Green qPCR SuperMix |
20-abx098033 |
Abbexa |
-
EUR 994.80
-
EUR 260.40
-
EUR 1362.00
-
EUR 493.20
|
|
|
Tip Green qPCR SuperMix |
20-abx098034 |
Abbexa |
-
EUR 1161.60
-
EUR 276.00
-
EUR 1596.00
-
EUR 560.40
|
|
|
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058200 |
Biochain |
200 reactions |
EUR 188 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058400 |
Biochain |
400 reactions |
EUR 316 |
Library Quantification qPCR SuperMix |
20-abx098896 |
Abbexa |
|
|
|
Top Green qPCR SuperMix (+Dye II) |
20-abx098890 |
Abbexa |
-
EUR 994.80
-
EUR 260.40
-
EUR 1362.00
-
EUR 493.20
|
|
|
Tip Green qPCR SuperMix (+Dye II) |
20-abx098891 |
Abbexa |
-
EUR 1161.60
-
EUR 276.00
-
EUR 1596.00
-
EUR 560.40
|
|
|
cDNA Synthesis SuperMix for qPCR |
20-abx09801920ulSystems |
Abbexa |
|
- 100 rxns ×20 ul Systems
- 50 rxns × 20 ul Systems
|
|
HyperScript RT SuperMix for qPCR |
K1074-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 350 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR |
K1074-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 188 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HiScript II Q RT SuperMix for qPCR |
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 282 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 861.6 |
|
HiScript II Q Select RT SuperMix for qPCR |
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 282 |
HiScript III RT SuperMix for qPCR (+g DNA wiper) |
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 351.6 |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-100 |
ApexBio |
100 rxns |
EUR 380 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-50 |
ApexBio |
50 rxns |
EUR 220 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054004 |
Biochain |
400 rxn |
EUR 635 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054200 |
Biochain |
200 reactions |
EUR 418 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054400 |
Biochain |
400 reactions |
EUR 635 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054002 |
Biochain |
200 rxn |
EUR 418 |
HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 292.8 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-01 |
Vazyme |
100 rxns (21 μl/rxn) |
EUR 174 |
PCR SuperMix |
20-abx098887 |
Abbexa |
|
|
|
PCR SuperMix (+dye) |
20-abx098003 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 7408.80
-
EUR 309.60
|
|
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PCR SuperMix (-dye) |
20-abx098002 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
|
|
|
Plus PCR SuperMix |
20-abx098888 |
Abbexa |
|
|
|
Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
T PCR SuperMix (-dye) |
20-abx098005 |
Abbexa |
|
|
|
T PCR SuperMix (+dye) |
20-abx098006 |
Abbexa |
|
|
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cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
EasyPfu PCR SuperMix (-dye) |
20-abx098009 |
Abbexa |
|
|
|
FastPfu PCR SuperMix (-dye) |
20-abx098010 |
Abbexa |
|
|
|
Library Amplification SuperMix |
20-abx098894 |
Abbexa |
|
|
|
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055004 |
Biochain |
400 rxn |
EUR 609 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055200 |
Biochain |
200 reactions |
EUR 381 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055400 |
Biochain |
400 reactions |
EUR 609 |
PCR SuperMix for PAGE (+dye) |
20-abx098004 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
|
|
|
OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 169.2 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055002 |
Biochain |
200 rxn |
EUR 380 |
OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
Green Two-Step qRT-PCR SuperMix |
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 777.6 |
|
Green One-Step qRT-PCR SuperMix |
20-abx09803820ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Probe One-Step qRT-PCR SuperMix |
20-abx09804220ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 727.2 |
|
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 910.8 |
|
All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 547.2 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1743.6 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
abx098027-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 727.2 |
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098028-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 794.4 |
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098029-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 1028.4 |
|
Fly First-Strand cDNA Synthesis SuperMix |
20-abx09885820ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
Green miRNA Two-Step qRT-PCR SuperMix |
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems |
Abbexa |
20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) |
EUR 1062 |
|
miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 878.4 |
|
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
20-abx098007 |
Abbexa |
|
|
|
Green Two-Step qRT-PCR SuperMix (GC Rich) |
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 961.2 |
|
Green One-Step qRT-PCR SuperMix (GC Rich) |
20-abx09803920ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Probe One-Step qRT-PCR SuperMix (GC Rich) |
20-abx09804120ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 693.6 |
|
HyperScript First-Strand cDNA Synthesis SuperMix |
K1073-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 352 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HyperScript First-Strand cDNA Synthesis SuperMix |
K1073-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 188 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
One-Step gDNA Removal and cDNA Synthesis SuperMix |
20-abx09885920ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
20-abx09801620ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
20-abx09802120ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
20-abx09802220ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
20-abx09801520ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
20-abx09801720ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
All-in-One First-Strand cDNA Synthesis SuperMix for PCR |
abx098023-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 878.4 |
|
HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper) |
K1173-100 |
ApexBio |
100 rxns |
EUR 380 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper) |
K1173-50 |
ApexBio |
50 rxns |
EUR 220 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA) |
20-abx098008 |
Abbexa |
|
|
|
Built in power supply for WSE-1165
Power AC 100-240V |
WSE-650-659 |
Atto |
1unit |
EUR 871.2 |
Description: Assembl y power suppl y wi t h WSE-1165 such as bui l t -i n |
electrophoresis power supply |
EV245 |
Consort |
ea |
EUR 574.8 |
Four Source Power Supply |
EDK621 |
Bio Basic |
1 UNIT |
EUR 821.08 |
|
Dual Source 150 Power Supply |
EDK620 |
Bio Basic |
1 UNIT |
EUR 445.75 |
|
POWER CORD KIT - RED/BLACK |
IB50500 |
IBI Scientific |
48 LONG - MALE TO FEMALE" |
EUR 77.93 |
POWER CORD KIT - RED/BLACK |
SPC-M4 |
IBI Scientific |
48 LONG - MALE TO MALE" |
EUR 114.67 |
CyanRed Power Box |
2305226 |
Atto |
2unit |
EUR 339.6 |
Description: i ndepent l y use for CyanRed Epi |
ENDURO™ 250V Power Supply , 120V |
LE0203-120 |
GenDepot |
Ea |
EUR 1923.6 |
ENDURO™ 250V Power Supply , 230V |
LE0203-230 |
GenDepot |
Ea |
EUR 1923.6 |
ENDURO™ 300V Power Supply , 120V |
LE0303-120 |
GenDepot |
Ea |
EUR 1437.6 |
myVolt™ Mini power supply, 115V |
BM0294 |
GenDepot |
Ea |
EUR 368.4 |
myVolt™ Mini power supply, 230V |
BM0295 |
GenDepot |
Ea |
EUR 368.4 |
myVolt™ Mini Power Supply,115V |
LE1007 |
GenDepot |
Ea |
EUR 368.4 |
myVolt™ Mini Power Supply, 230V |
LE1008 |
GenDepot |
Ea |
EUR 368.4 |
electrophoresis power supply 120 vac |
EV245-US |
Consort |
ea |
EUR 574.8 |
myVolt™ Touch power supply, 115V |
BM0296 |
GenDepot |
Ea |
EUR 861.6 |
myVolt™ Touch power supply, 230V |
BM0297 |
GenDepot |
Ea |
EUR 861.6 |
myVolt™ Touch power supply, 115V |
LE1009 |
GenDepot |
Ea |
EUR 861.6 |
myVolt™ Touch power supply, 220V |
LE1010 |
GenDepot |
Ea |
EUR 861.6 |
electrophoresis power supply uk plug |
EV245-UK |
Consort |
ea |
EUR 574.8 |
Power supply for LABeler Lab Printer |
L9010-PS |
MTC Bio |
1/pack |
EUR 100.32 |
Description: Power supply for Labeler |
UNIVERSAL POWER SUPPLY FOR AXYGEN MOTOPET |
MOTO-SP-PWR |
CORNING |
1/pk |
EUR 73.2 |
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs |
LABeler Lab Printer w/ 115V power supply |
L9010 |
MTC Bio |
1/pack |
EUR 224.76 |
Description: LABeler Hand Held Labeler |
REPLACEMENT POWER CORD - 115V |
COMCD0002 |
IBI Scientific |
6FT - FLAT |
EUR 27.48 |
REPLACEMENT POWER CORD - 230V |
COMCD0004 |
IBI Scientific |
6FT - EUROPEAN PLUG |
EUR 20.62 |
REPLACEMENT POWER CORD - 230V |
COMCD0005 |
IBI Scientific |
8FT. - UK STYLE PLUG (BS1363) |
EUR 42.2 |
CometAssay 20 Well ES Starter Kit plus NA Power Supply |
4252-040-ESK-PS1 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay 20 Well ES Starter Kit plus EU Power Supply |
4252-040-ESK-PS2 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay 20 Well ES Starter Kit plus SZ Power Supply |
4252-040-ESK-PS4 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay 96 Well ES Starter Kit plus NA Power Supply |
4253-096-ESK-PS1 |
Biotechne |
1 Kit |
EUR 4528.8 |
CometAssay 96 Well ES Starter Kit plus EU Power Supply |
4253-096-ESK-PS2 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay 96 Well ES Starter Kit plus UK Power Supply |
4253-096-ESK-PS3 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay 96 Well ES Starter Kit plus SZ Power Supply |
4253-096-ESK-PS4 |
Biotechne |
1 Kit |
EUR 4597.68 |
Accuris PowerVolt™ High Current, 300V/2000mA Power Supply, 115V |
E2302-2000 |
Benchmark Scientific |
1 each |
EUR 1333.8 |
Accuris PowerVolt™ High Current, 300V/2000mA Power Supply, 230V |
E2302-2000-E |
Benchmark Scientific |
1 each |
EUR 1333.8 |
CometAssay Electrophoresis Starter Kit plus EU Power Supply |
4250-050-ESK-PS2 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus UK Power Supply |
4250-050-ESK-PS3 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus SZ Power Supply |
4250-050-ESK-PS4 |
Biotechne |
1 Kit |
EUR 4597.68 |
Animal Detection Probe qPCR Super PreMix |
QV114-EN01 |
Vazyme |
400 rxns |
EUR 128 |
Animal Detection Probe qPCR Super PreMix |
QV114-EN02 |
Vazyme |
800 rxns |
EUR 230.4 |
CometAssay 20 Well ES Starter Kit plus AUS Power Supply |
4252-040-ESK-PS5 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay 96 Well ES Starter Kit plus AUS Power Supply |
4253-096-ESK-PS5 |
Biotechne |
1 Kit |
EUR 4597.68 |
Alkaline phosphatase Power AP |
AZ140A |
Bio Basic |
1MG |
EUR 120.55 |
|
Alkaline phosphatase Power AP |
AZ235A |
Bio Basic |
1MG |
EUR 139.34 |
|
ENDURO™ Mini 300V Power Supply , 110V-220V |
LE0304 |
GenDepot |
Ea |
EUR 846 |
CometAssay 20 Well ES Starter Kit plus Power Supply for UK |
4252-040-ESK-PS3 |
Biotechne |
1 Kit |
EUR 4597.68 |
Power leads for HorizeBLOT-R |
2322462 |
Atto |
5unit |
EUR 324 |
SEMI-DRY BLOTTER POWER CORDS |
IB45500 |
IBI Scientific |
RED/BLK - 36",0.0000,0.0000,0.0000,0.0000,0.0000,0.0000,",N" |
EUR 122.26 |
PIPET CONTROLLER - UNIVERSAL POWER SUPPLY IN:100-250V, OUT: |
357489 |
CORNING |
1/pk |
EUR 69.6 |
Description: Falcon Liquid Handling Equipment; Falcon Pipet Controllers and Accessories |
AXYGEN® POWER SUPPLY ADAPTERS, 4 MM TO 4 MM, 2/PK |
HGB7-AD |
CORNING |
1/pk |
EUR 50.4 |
Description: Lab Equipment; Axygen Branded EQ |
AXYGEN® POWER SUPPLY ADAPTERS, 4 MM TO 2 MM, 2/PK |
HGB7-AD2 |
CORNING |
1/pk |
EUR 50.4 |
Description: Lab Equipment; Axygen Branded EQ |
Replacement power supply for nUVaClean UV Pipette Carousel |
P5590-PS |
MTC Bio |
1/pack |
EUR 91.6 |
Description: Replacement power supply for nUVaClean UV Pipette Carousel |
OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit |
STA-859 |
Cell Biolabs |
200 assays |
EUR 609.6 |
Description: OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within various samples. Following the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the sample, the kit colorimetric probe develops a blue color that is read colorimetrically at 540-600nm. The antioxidant potential of samples is determined based on an iron standard curve and results are calculated at Fe2+ equivalents (µM) or FRAP value. |
Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric) |
K515-200 |
Biovision |
each |
EUR 660 |
Power Broth™, Single Packs - box of 10 |
0106-S |
AthenaES |
1L each |
EUR 123.6 |
UNIVERSAL POWER SUPPLY, 9V, WITH SET OF ADAPTORS EU, UK, US, AUS |
4996 |
CORNING |
1/pk |
EUR 69.6 |
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs |
HotTaq Power Mix Ready-to-Load 12.5 mM |
BT10801 |
Bioatlas |
250rxn |
EUR 147.6 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
LinkLabel BlueTooth Enabled Label Printer w/ 115-230V power supply |
L3000 |
MTC Bio |
1/pack |
EUR 350.63 |
Description: LinkLabel Bluetooth Enabled Labeler |
Power Combo Value Pack - Morpheus and MIDASplus |
M-MD1-76 |
MiTeGen |
192 x 1 ml ml |
EUR 1675 |
Description: Power Combo Value Pack - Morpheus and MIDASplus |
myGel InstaView Package: E1200 and E2301 touch screen power supply |
E1200-PSP |
Benchmark Scientific |
1 each |
EUR 1276.6 |
Safety Cover - AE-6500(6450) - Without power leads |
2398244 |
Atto |
4unit |
EUR 315.6 |
Power Prime Broth™, Single Packs - box of 10 |
0112-S |
AthenaES |
1L each |
EUR 133.2 |
FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit (2 Plate) |
K043-H1 |
Arbor Assays |
2 x 96 well plate |
EUR 554 |
Power Prime-olate™, Single Packs - box of 10 |
0162-S |
AthenaES |
1 L each |
EUR 127.2 |
Power Combo Value Pack - Morpheus and MIDASplus HT96 |
M-MD1-76-HT |
MiTeGen |
2 x (96 x 10 ml) ml |
EUR 413 |
Description: Power Combo Value Pack - Morpheus and MIDASplus HT96 |
myGel InstaView Package: E1200 and E2301 touch screen power supply, 230V input |
E1200-PSP-E |
Benchmark Scientific |
1 each |
EUR 1282 |
CORNING® LSE™ MINI MICROCENTRIFUGE 24V POWER ADAPTER FOR 6770 |
401025 |
CORNING |
1/pk |
EUR 86.4 |
Description: Lab Equipment; General Purpose Centrifuges (affliated brand) |
HotTaq EvaGreen qPCR Mix (ROX) |
BT11101 |
Bioatlas |
250rxn |
EUR 147.6 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
Fast EvaGreen Master Mix for qPCR (5000 rxn) |
31003-2 |
Biotium |
50x1ML |
EUR 3739.2 |
Description: Minimum order quantity: 1 unit of 50x1ML |
HotTaq EvaGreen qPCR Mix (no ROX) |
BT11102 |
Bioatlas |
250rxn |
EUR 147.6 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Mix (Capillary) |
BT11103 |
Bioatlas |
250rxn |
EUR 147.6 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
Though the sensitivity of the brand new HTS pipeline was decrease than the particular qPCR, it was in a position to detect all kinds of fungal pathogens. FungiSearch is simple to make use of, and the reference database is updatable, making the device appropriate for speedy identification of recent pathogens. This new strategy combining spore trapping and HTS detection is promising as a diagnostic device for invasive fungal pathogens.
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