Analysis of the cox1 gene in Echinococcus granulosus from sheep in northeast Iran using PCR high-resolution melting (qPCR-HRM) curve analysis
Echinococcus granulosus, the etiologic agent of echinococcosis, is among the most essential zoonotic helminthes worldwide. Information of E. granulosus species and genotypes has essential implications for epidemiology, management, and prevention of illnesses in addition to future vaccine and drug designs. There are a lot of molecular strategies developed to outline genotypes of E. granulosus, amongst them excessive decision melting (HRM) evaluation, as a brand new strategy, is a single step and closed tube methodology. It’s acceptable for quick screening of huge variety of isolates. This method is an correct, consumer pleasant, cost-effective, quick and easy methodology, which doesn’t want post-PCR processes.
Between March and lst august 2016, of 726 sheep examined in abattoirs in Razavi Khorasan province, Northeast Iran, 109 harboured cystic echincoccosis lesions (liver samples= 65 and lung samples= 44) which have been collected for evaluation. Complete genomic DNA was extracted from every pattern and amplified for the presence of polymorphism within the mitochondrial cox1 gene of Echinococcus granulosus utilizing a excessive decision melting curve (HRM) methodology.
A complete of 109 hydatid cyst samples analyzed by PCR high-resolution melting (qPCR-HRM) curve of the cox1 gene, all isolates have been recognized as G1 genotype (sheep pressure). G1 is the predominant genotype in sheep in northeast of Iran. The excessive incidence of the G1 genotype (identified to be the predominant E. granulosus genotype infecting people globally) in sheep has appreciable implications for hydatid illness management packages on this space.
Improvement and validation of a multiplex qPCR assay for detection and relative quantification of HPV16 and HPV18 E6 and E7 oncogenes
Human papillomaviruses (HPV) play a key function in selling human anogenital cancers. Present high-risk HPV screening or analysis exams contain cytological or molecular methods largely primarily based on qualitative HPV DNA detection. Right here, we describe the event of a speedy quantitative polymerase chain response (qPCR) detection take a look at of HPV16 and HPV18 oncogenes (E6 and E7) normalized on human gene encoding GAPDH. Optimized qPCR parameters have been outlined, and analytical specificities have been validated. The restrict of detection was 101 for all genes examined.
Assay performances have been evaluated on medical samples (n = 96). Concordance between the Xpert HPV assay and the triplex assay developed right here was 93.44% for HPV16 and 73.58% for HPV18. HPV co-infections have been detected in 15 samples. The programs developed within the current examine can be utilized in complement to conventional HPV exams for particularly validating the presence of HPV16 and/or HPV18. It will also be used for the follow-up of sufferers with confirmed an infection and liable to creating lesions, by means of the quantification of E6 and E7 oncogene expression (mRNA) normalized on the GAPDH expression ranges.
A multiplex TaqMan qPCR assay for detection and quantification of clade 1 and clade 2 isolates of Pseudoperonospora cubensis and Pseudoperonospora humuli
The flexibility to detect and quantify aerially dispersed plant pathogens is crucial for creating efficient illness management measures and epidemiological fashions that optimize the timing for management . There’s an acute want for managing the downy mildew pathogens infecting cucurbits and hop incited by members of the genus Pseudoperonospora (P. cubensis clade 1 and a couple of isolates and P. humuli, respectively).
A extremely particular multiplex TaqMan qPCR assay concentrating on distinctive sequences within the pathogens’ mitochondrial genomes was developed that permits detection of all three taxa in a single multiplexed amplification. An inside management included within the response evaluated if outcomes have been influenced by PCR inhibitors that may make it by means of the DNA extraction course of.
Dependable quantification of inoculum as little as three sporangia in a pattern was noticed. The multiplexed assay was examined with DNA extracted from purified sporangia, contaminated plant tissue and environmental samples collected on impaction spore traps samplers. The flexibility to precisely detect and concurrently quantify all three pathogens in a single multiplexed amplification ought to enhance administration choices for controlling the illnesses they trigger.

Identification and validation of reference genes for dependable evaluation of differential gene expression throughout antibiotic induced persister formation in Klebsiella pneumoniae utilizing qPCR
The examine of differential gene expression in persister cells is compounded by ceasure of typical mobile metabolic pathways throughout persistence. There’s, therefore, a requirement to establish and validate appropriate reference genes whose expression stays secure throughout persistence. We evaluated the suitability of 5 genes viz. dnaJ, groEL, rpoB, kp751, kp4432 as references to check gene expression utilizing real-time polymerase chain response (qPCR) throughout persister cell formation in Klebsiella pneumoniae. Outcomes obtained confirmed that whereas dnaJ and groEL suffered from unstable expression; rpoB, kp751 and kp4432 confirmed secure expression.
Additional, it was noticed that information normalization utilizing both of the secure genes viz. rpoB, kp751, kp4432 alone, resulted in both too low expression ranges or too excessive variation amongst replicates. Our examine signifies the concurrent use of kp4432 and rpoB as reference genes to be probably the most appropriate for dependable evaluation of differential gene expression throughout antibiotic induced persister formation in Okay. pneumoniae. kp4432 and rpoB encode NAD-dependant phenylacetaldehyde dehydrogenase and DNA-directed RNA polymerase beta subunit respectively.
The result of this examine will enhance the utility of qPCR in finding out the temporal adjustments in gene expression throughout persistence. The examine can even help in understanding mechanisms underlying persister cell formation notably in Okay. pneumoniae.
Comparability of qPCR and Metabarcoding Strategies as Instruments for the Detection of Airborne Inoculum of Forest Fungal Pathogens
Forest illnesses brought on by invasive fungal pathogens have gotten extra widespread, typically with dramatic penalties to forest ecosystems. The event of early detection programs is important for environment friendly surveillance and to mitigate the impression of invasive pathogens. Windborne spores are an essential pathway for introduction of fungal pathogens into new areas; the design of spore trapping units tailored to forests, able to gathering various kinds of spores, and aligned with improvement of environment friendly molecular strategies for detection of the pathogen, ought to assist forest managers anticipate new illness outbreaks.
Two varieties of Rotorod samplers have been evaluated for the gathering of airborne inoculum of forest fungal pathogens with a variety of spore sizes in 5 forest varieties. Detection was by particular quantitative PCR (qPCR) and by high-throughput sequencing (HTS) of amplified inside transcribed spacer sequences utilizing a brand new bioinformatic pipeline, FungiSearch, developed for diagnostic functions. Validation of the pipeline was performed on mock communities of 10 fungal species belonging to totally different taxa.
Pro QPCR SuperMix Kit |
K5053200 |
Biochain |
200 reactions |
EUR 188 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit |
K5053400 |
Biochain |
400 reactions |
EUR 299 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Green qPCR SuperMix UDG |
20-abx098032 |
Abbexa |
-
EUR 1135.00
-
EUR 453.00
-
EUR 662.00
|
|
|
Top Green qPCR SuperMix |
20-abx098033 |
Abbexa |
-
EUR 829.00
-
EUR 217.00
-
EUR 1135.00
-
EUR 411.00
|
|
|
Tip Green qPCR SuperMix |
20-abx098034 |
Abbexa |
-
EUR 968.00
-
EUR 230.00
-
EUR 1330.00
-
EUR 467.00
|
|
|
Library Quantification qPCR SuperMix |
20-abx098896 |
Abbexa |
|
|
|
Pro QPCR SuperMix Kit - ROX premixed |
K5056200 |
Biochain |
200 reactions |
EUR 188 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit - ROX premixed |
K5056400 |
Biochain |
400 reactions |
EUR 299 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
cDNA Synthesis SuperMix for qPCR |
20-abx09801920ulSystems |
Abbexa |
|
-
100 rxns ×20 ul Systems
-
50 rxns × 20 ul Systems
|
|
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054200 |
Biochain |
200 reactions |
EUR 408 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054400 |
Biochain |
400 reactions |
EUR 594 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058200 |
Biochain |
200 reactions |
EUR 211 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058400 |
Biochain |
400 reactions |
EUR 320 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Top Green qPCR SuperMix (+Dye II) |
20-abx098890 |
Abbexa |
-
EUR 829.00
-
EUR 217.00
-
EUR 1135.00
-
EUR 411.00
|
|
|
Tip Green qPCR SuperMix (+Dye II) |
20-abx098891 |
Abbexa |
-
EUR 968.00
-
EUR 230.00
-
EUR 1330.00
-
EUR 467.00
|
|
|
HiScript II Q RT SuperMix for qPCR |
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 718 |
|
HiScript II Q Select RT SuperMix for qPCR |
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
HS-100-QPCR |
CORNING |
100/pk |
EUR 318 |
Description: Sealing Products; Sealing films - Axygen |
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
HS-150-QPCR |
CORNING |
100/pk |
EUR 318 |
Description: Sealing Products; Sealing films - Axygen |
HiScript III RT SuperMix for qPCR (+g DNA wiper) |
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 293 |
HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 244 |
PCR SuperMix |
20-abx098887 |
Abbexa |
|
|
|
OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 89 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 141 |
Evo? cDNA Supermix |
M1168-100 |
Biovision |
|
EUR 381 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
|
EUR 267 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
|
EUR 441 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
|
EUR 289 |
PCR SuperMix (-dye) |
20-abx098002 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
|
|
|
PCR SuperMix (+dye) |
20-abx098003 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 6174.00
-
EUR 258.00
|
|
|
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
|
|
Plus PCR SuperMix |
20-abx098888 |
Abbexa |
|
|
|
Library Amplification SuperMix |
20-abx098894 |
Abbexa |
|
|
|
CyanRed Power Box |
2305226 |
Atto |
2unit |
EUR 283 |
Description: i ndepent l y use for CyanRed Epi |
electrophoresis power supply |
EV245 |
Consort |
ea |
EUR 479 |
COLLECTION CONTAINER 1L, EVA FILM |
91-200-01 |
CORNING |
1/pk |
EUR 80 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 2L, EVA FILM |
91-200-02 |
CORNING |
1/pk |
EUR 88 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
91-200-05 |
CORNING |
1/pk |
EUR 99 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-10 |
CORNING |
1/pk |
EUR 101 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-20 |
CORNING |
1/pk |
EUR 110 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-36 |
CORNING |
1/pk |
EUR 86 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-39 |
CORNING |
1/pk |
EUR 89 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 1L, EVA FILM |
91-200-42 |
CORNING |
1/pk |
EUR 73 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
91-200-43 |
CORNING |
1/pk |
EUR 151 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-45 |
CORNING |
1/pk |
EUR 126 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-47 |
CORNING |
1/pk |
EUR 132 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 50L, EVA FILM |
91-200-48 |
CORNING |
1/pk |
EUR 146 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 100L, EVA FILM |
91-200-82 |
CORNING |
1/pk |
EUR 279 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 200L, EVA FILM |
91-200-83 |
CORNING |
1/pk |
EUR 292 |
Description: Flexible Containers; Bioprocess Bags and Parts |
qPCR Control Template Kit |
G929 |
ABM |
100 rxns |
EUR 148 |
Lentivirus qPCR Quantification Kit |
K1471-100 |
Biovision |
100 Rxns |
EUR 298 |
Retrovirus qPCR Quantification Kit |
K1472-100 |
Biovision |
100 Rxns |
Ask for price |
QPCR Positive Control Kit |
M1126-100 |
Biovision |
|
EUR 403 |
OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 169 |
T PCR SuperMix (-dye) |
20-abx098005 |
Abbexa |
|
|
|
T PCR SuperMix (+dye) |
20-abx098006 |
Abbexa |
|
|
|
EasyPfu PCR SuperMix (-dye) |
20-abx098009 |
Abbexa |
|
|
|
FastPfu PCR SuperMix (-dye) |
20-abx098010 |
Abbexa |
|
|
|
12v vehicle power adapter |
BSH100-A12 |
BenchMark |
1 PC |
EUR 429.53 |
|
Alkaline phosphatase Power AP |
AZ140A |
Bio Basic |
1MG |
EUR 100.46 |
|
Alkaline phosphatase Power AP |
AZ235A |
Bio Basic |
1MG |
EUR 116.12 |
|
Four Source Power Supply |
EDK621 |
Bio Basic |
1 UNIT |
EUR 684.23 |
|
COLLECTION CONTAINER 500 ML, EVA FILM |
91-200-41 |
CORNING |
1/pk |
EUR 69 |
Description: Flexible Containers; Bioprocess Bags and Parts |
Methylamp MS-qPCR Fast Kit |
P-1028 |
EpiGentek |
200 Reactions |
EUR 496.6 |
Description: fast delivery possible |
Universal qPCR Mixture |
QPCR1000-UNIV |
Bio Basic |
4X1.25ml |
EUR 276.2 |
|
Built in power supply for WSE-1165
Power AC 100-240V |
WSE-650-659 |
Atto |
1unit |
EUR 726 |
Description: Assembl y power suppl y wi t h WSE-1165 such as bui l t -i n |
PCR SuperMix for PAGE (+dye) |
20-abx098004 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
|
|
|
Frit Kit |
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
RA900A-1 |
SBI |
20 profiles |
EUR 850 |
|
Column Packing Kit |
PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
Power leads for HorizeBLOT-R |
2322462 |
Atto |
5unit |
EUR 270 |
electrophoresis power supply uk plug |
EV245-UK |
Consort |
ea |
EUR 479 |
electrophoresis power supply 120 vac |
EV245-US |
Consort |
ea |
EUR 479 |
Dual Source 150 Power Supply |
EDK620 |
Bio Basic |
1 UNIT |
EUR 371.46 |
|
PCR Mycoplasma Detection Kit |
M034-Kit |
TOKU-E |
Kit |
EUR 266 |
Protein Eva-1 Homolog C (EVA1C) Antibody |
20-abx302696 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055200 |
Biochain |
200 reactions |
EUR 376 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055400 |
Biochain |
400 reactions |
EUR 572 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
RA930A-1 |
SBI |
20 profiles |
EUR 850 |
|
Coronavirus Rapid RT-qPCR Detection Kit |
K1461-100 |
Biovision |
100 Rxns |
EUR 551 |
Adeno-Associated Virus qPCR Quantification Kit |
K1473-100 |
Biovision |
100 Rxns |
EUR 348 |
All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 456 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1453 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 732 |
|
Green Two-Step qRT-PCR SuperMix |
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 648 |
|
Green One-Step qRT-PCR SuperMix |
20-abx09803820ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
|
|
Probe One-Step qRT-PCR SuperMix |
20-abx09804220ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
|
|
Fly First-Strand cDNA Synthesis SuperMix |
20-abx09885820ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
|
|
Neural Lineage qPCR Profiler |
RA500A-1 |
SBI |
10 reactions |
EUR 999 |
|
Cancer MicroRNA qPCR Array |
RA610A-1 |
SBI |
10 profiles |
EUR 1172 |
|
Regulatory RNA qPCR Profiler |
RA950A-1 |
SBI |
20 rxn |
EUR 880 |
|
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1110 |
|
Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric) |
K515-200 |
Biovision |
|
EUR 550 |
OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit |
STA-859 |
Cell Biolabs |
200 assays |
EUR 508 |
Description: OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within various samples. Following the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the sample, the kit colorimetric probe develops a blue color that is read colorimetrically at 540-600nm. The antioxidant potential of samples is determined based on an iron standard curve and results are calculated at Fe2+ equivalents (µM) or FRAP value. |
CometAssay Electrophoresis Starter Kit plus EU Power Supply |
4250-050-ESK-PS2 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay Electrophoresis Starter Kit plus UK Power Supply |
4250-050-ESK-PS3 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay Electrophoresis Starter Kit plus SZ Power Supply |
4250-050-ESK-PS4 |
Biotechne |
1 Kit |
EUR 3831.4 |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV100PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV105PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV120PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV125PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS700A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS720A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS740A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
CAS510A-KIT |
SBI |
1 Kit |
EUR 805 |
|
Power supply for LABeler Lab Printer |
L9010-PS |
MTC Bio |
1/pack |
EUR 83.6 |
Description: Power supply for Labeler |
ENDURO™ 250V Power Supply , 120V |
LE0203-120 |
GenDepot |
Ea |
EUR 1603 |
ENDURO™ 250V Power Supply , 230V |
LE0203-230 |
GenDepot |
Ea |
EUR 1603 |
ENDURO™ 300V Power Supply , 120V |
LE0303-120 |
GenDepot |
Ea |
EUR 1198 |
myVolt™ Mini Power Supply,115V |
LE1007 |
GenDepot |
Ea |
EUR 307 |
myVolt™ Mini Power Supply, 230V |
LE1008 |
GenDepot |
Ea |
EUR 307 |
myVolt™ Touch power supply, 115V |
LE1009 |
GenDepot |
Ea |
EUR 718 |
myVolt™ Touch power supply, 220V |
LE1010 |
GenDepot |
Ea |
EUR 718 |
myVolt™ Mini power supply, 115V |
BM0294 |
GenDepot |
Ea |
EUR 307 |
myVolt™ Mini power supply, 230V |
BM0295 |
GenDepot |
Ea |
EUR 307 |
myVolt™ Touch power supply, 115V |
BM0296 |
GenDepot |
Ea |
EUR 718 |
myVolt™ Touch power supply, 230V |
BM0297 |
GenDepot |
Ea |
EUR 718 |
UNIVERSAL POWER SUPPLY FOR AXYGEN MOTOPET |
MOTO-SP-PWR |
CORNING |
1/pk |
EUR 61 |
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs |
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV200PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV205PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV220PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV225PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS750A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS770A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS790A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
CAS9LIG-KIT |
SBI |
1 Kit |
EUR 153 |
|
One.Step RT-qPCR Probe Kit (2X concentrated) |
105-500 |
GeneOn |
2 x 1,25 ml |
EUR 458 |
One.Step RT-qPCR Probe Kit (2X concentrated) |
105-502 |
GeneOn |
10 x 1,25 ml |
EUR 1719 |
piggyBac qPCR copy number kit, 20 reactions |
PBC100A-1 |
SBI |
20 reactions |
EUR 331 |
|
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
PIN320A-KIT |
SBI |
1 Kit |
EUR 4941 |
|
First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 578 |
|
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 606 |
|
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 759 |
|
Green miRNA Two-Step qRT-PCR SuperMix |
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems |
Abbexa |
20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) |
EUR 885 |
|
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
PIN340iPS-KIT |
SBI |
1 Kit |
EUR 4941 |
|
FRAP? (Ferric Reducing Antioxidant Power) Detection Kit (2 Plate) |
K043-H1 |
Arbor Assays |
2 x 96 well plate |
EUR 496 |
BrightGreen 5X qPCR MasterMix-iCycler |
MasterMix-5C |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-ROX |
MasterMix-5P |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-iCycler |
MasterMix-5PC |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-Multiplex |
MasterMix-5PM |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
BrightGreen 5X qPCR MasterMix-ROX |
MasterMix-5R |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
BrightGreen 2X qPCR MasterMix-iCycler |
MasterMix-iC |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-iCycler |
MasterMix-iC-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 376 |
KiloGreen 2X qPCR MasterMix-iCycler |
MasterMix-KC |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
KiloGreen 2X qPCR MasterMix-ROX |
MasterMix-KR |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
BrightGreen miRNA qPCR MasterMix-iCycler |
MasterMix-mC |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
BrightGreen miRNA qPCR MasterMix-ROX |
MasterMix-mR |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
TaqProbe 2X qPCR MasterMix-ROX |
MasterMix-P |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
TaqProbe 2X qPCR MasterMix-iCycler |
MasterMix-PC |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
TaqProbe 2X qPCR MasterMix-Multiplex |
MasterMix-PM |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-ROX |
MasterMix-R |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-ROX |
MasterMix-R-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 376 |
AceQ qPCR Probe Master Mix |
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 221 |
AceQ qPCR Probe Master Mix |
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 646 |
HotTaq Probe qPCR Mix (ROX) |
BT11001 |
Bioatlas |
250rxn |
EUR 109 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq Probe qPCR Mix (Capillary) |
BT11003 |
Bioatlas |
250rxn |
EUR 109 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq Probe qPCR Universal Mix |
BT11004 |
Bioatlas |
250rxn |
EUR 123 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Mix (ROX) |
BT11101 |
Bioatlas |
250rxn |
EUR 123 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Mix (Capillary) |
BT11103 |
Bioatlas |
250rxn |
EUR 123 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Universal Mix |
BT11104 |
Bioatlas |
250rxn |
EUR 138 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
SYBR Green qPCR Master Mix |
HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 263 |
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 291 |
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
RA910A-1 |
SBI |
20 profiles |
EUR 1108 |
|
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE620A-KIT |
SBI |
1 kit |
EUR 2132 |
|
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE622A-KIT |
SBI |
1 kit |
EUR 2132 |
|
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE624A-KIT |
SBI |
1 kit |
EUR 2132 |
|
LABeler Lab Printer w/ 115V power supply |
L9010 |
MTC Bio |
1/pack |
EUR 187.3 |
Description: LABeler Hand Held Labeler |
One.Step RT-qPCR Kit with EvaGreen (2X concentrated) |
105-520 |
GeneOn |
2 x 1,25 ml |
EUR 458 |
One.Step RT-qPCR Kit with EvaGreen (2X concentrated) |
105-522 |
GeneOn |
10 x 1,25 ml |
EUR 1719 |
igScript One Step RT-qPCR Kit - 100 Reactions |
4214 |
Intact Genomics |
1/EA |
EUR 253 |
igScript One Step RT-qPCR Kit - 500 Reactions |
4218 |
Intact Genomics |
1/EA |
EUR 773 |
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
20-abx098007 |
Abbexa |
|
|
|
One-Step RT-PCR SuperMix (+dye) (4 kb) |
abx098027-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 606 |
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098028-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 662 |
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098029-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 857 |
|
Green Two-Step qRT-PCR SuperMix (GC Rich) |
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 801 |
|
Green One-Step qRT-PCR SuperMix (GC Rich) |
20-abx09803920ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
|
|
Probe One-Step qRT-PCR SuperMix (GC Rich) |
20-abx09804120ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
|
|
One-Step gDNA Removal and cDNA Synthesis SuperMix |
20-abx09885920ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
|
|
vWF Acty. Kit |
ABP-ACT-KIT |
Abpcorp |
12 x 8 microwells |
EUR 428 |
vWF Ant. Kit |
ABP-TOT-KIT |
Abpcorp |
12 x 8 microwells |
EUR 394 |
CometAssay 20 Well ES Starter Kit plus NA Power Supply |
4252-040-ESK-PS1 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay 20 Well ES Starter Kit plus EU Power Supply |
4252-040-ESK-PS2 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay 20 Well ES Starter Kit plus SZ Power Supply |
4252-040-ESK-PS4 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay 20 Well ES Starter Kit plus AUS Power Supply |
4252-040-ESK-PS5 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay 96 Well ES Starter Kit plus NA Power Supply |
4253-096-ESK-PS1 |
Biotechne |
1 Kit |
EUR 3774 |
CometAssay 96 Well ES Starter Kit plus EU Power Supply |
4253-096-ESK-PS2 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay 96 Well ES Starter Kit plus UK Power Supply |
4253-096-ESK-PS3 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay 96 Well ES Starter Kit plus SZ Power Supply |
4253-096-ESK-PS4 |
Biotechne |
1 Kit |
EUR 3831.4 |
CometAssay 96 Well ES Starter Kit plus AUS Power Supply |
4253-096-ESK-PS5 |
Biotechne |
1 Kit |
EUR 3831.4 |
Opti-Seal qPCR and Storage Films |
G495 |
ABM |
100 Films/Box |
EUR 202 |
Opti-Seal qPCR 384 Well Plate |
G935 |
ABM |
10 Plates/Box |
EUR 89 |
Opti-Seal qPCR 384 Well Plate |
G936 |
ABM |
100 Plates/Case |
EUR 428 |
2x SYBR Green qPCR Master Mix |
B21202 |
Bimake |
5 mL |
EUR 224 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2x SYBR Green qPCR Master Mix |
B21203 |
Bimake |
25 mL |
EUR 856 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
BrightGreen 5X qPCR MasterMix-Low ROX |
MasterMix-5L |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-Low ROX |
MasterMix-5PL |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-No Dye |
MasterMix-5PS |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
Though the sensitivity of the brand new HTS pipeline was decrease than the particular qPCR, it was in a position to detect all kinds of fungal pathogens. FungiSearch is simple to make use of, and the reference database is updatable, making the device appropriate for speedy identification of recent pathogens. This new strategy combining spore trapping and HTS detection is promising as a diagnostic device for invasive fungal pathogens.
Tags :
dna,
dna template synthesis,
dna template synthesis of polymers,
dna templates google slides,
dna test for dogs,
dna testing center near me,
dna testing comparison chart,
dna testing cost,
dna testing ethics,
dna testing for ancestry,
dna testing for birds,
dna testing for cats,
dna testing for dogs,
dna testing for health,
dna testing for paternity,
dna testing fraud,
dna testing free online,
dna testing heritage,
dna testing kits,
dna testing kits 2020,
dna testing kits for paternity,
dna testing kits for paternity hair,
dna testing kits guides,
dna testing kits/fl,
dna testing lab,
dna testing sites,
dna testing song,
dna testing while pregnant,
dna tests,
dnajlion7,
dnajlion7 youtube,
dnalion7 youtube,
pcr,
pcr covid test,
pcr covid testing,
pcr kits price,
pcr test for coronavirus,
pcr test for covid 19,
pcr testing for covid-19,
pcr tests,
pcr unit,
pcram,
pcrb,
pcrdfans,
pcre,
pcrfy,
pcrfy stock,
pcrichard credit card pay bill,
pcrichard pay bill,
pcrichard&sons,
pcrichard.com,
pcrichardsandsons,
pcrm,
pcrx stock,
peptides and acids,
peptides anti-aging,
peptides collagen powder,
peptides definition,
peptides diet,
peptides for bodybuilding,
peptides for erectile dysfunction,
peptides for eyelash growth,
peptides for nerve repair,
peptides for sale,
peptides for skin,
peptides for weight loss,
peptides international,
peptides rodan and fields,
peptides serum,
peptides supplement