MicroarrayWorld Microarrays and gene expression profiling

Analysis of the cox1 gene in Echinococcus granulosus from sheep in northeast Iran using PCR high-resolution melting (qPCR-HRM) curve analysis

Echinococcus granulosus, the etiologic agent of echinococcosis, is among the most essential zoonotic helminthes worldwide. Information of E. granulosus species and genotypes has essential implications for epidemiology, management, and prevention of illnesses in addition to future vaccine and drug designs. There are a lot of molecular strategies developed to outline genotypes of E. granulosus, amongst them excessive decision melting (HRM) evaluation, as a brand new strategy, is a single step and closed tube methodology. It’s acceptable for quick screening of huge variety of isolates. This method is an correct, consumer pleasant, cost-effective, quick and easy methodology, which doesn’t want post-PCR processes.

Between March and lst august 2016, of 726 sheep examined in abattoirs in Razavi Khorasan province, Northeast Iran, 109 harboured cystic echincoccosis lesions (liver samples= 65 and lung samples= 44) which have been collected for evaluation. Complete genomic DNA was extracted from every pattern and amplified for the presence of polymorphism within the mitochondrial cox1 gene of Echinococcus granulosus utilizing a excessive decision melting curve (HRM) methodology.

A complete of 109 hydatid cyst samples analyzed by PCR high-resolution melting (qPCR-HRM) curve of the cox1 gene, all isolates have been recognized as G1 genotype (sheep pressure). G1 is the predominant genotype in sheep in northeast of Iran. The excessive incidence of the G1 genotype (identified to be the predominant E. granulosus genotype infecting people globally) in sheep has appreciable implications for hydatid illness management packages on this space.

Improvement and validation of a multiplex qPCR assay for detection and relative quantification of HPV16 and HPV18 E6 and E7 oncogenes

Human papillomaviruses (HPV) play a key function in selling human anogenital cancers. Present high-risk HPV screening or analysis exams contain cytological or molecular methods largely primarily based on qualitative HPV DNA detection. Right here, we describe the event of a speedy quantitative polymerase chain response (qPCR) detection take a look at of HPV16 and HPV18 oncogenes (E6 and E7) normalized on human gene encoding GAPDH. Optimized qPCR parameters have been outlined, and analytical specificities have been validated. The restrict of detection was 10¹ for all genes examined.

Assay performances have been evaluated on medical samples (n = 96). Concordance between the Xpert HPV assay and the triplex assay developed right here was 93.44% for HPV16 and 73.58% for HPV18. HPV co-infections have been detected in 15 samples. The programs developed within the current examine can be utilized in complement to conventional HPV exams for particularly validating the presence of HPV16 and/or HPV18. It will also be used for the follow-up of sufferers with confirmed an infection and liable to creating lesions, by means of the quantification of E6 and E7 oncogene expression (mRNA) normalized on the GAPDH expression ranges.

A multiplex TaqMan qPCR assay for detection and quantification of clade 1 and clade 2 isolates of Pseudoperonospora cubensis and Pseudoperonospora humuli

The flexibility to detect and quantify aerially dispersed plant pathogens is crucial for creating efficient illness management measures and epidemiological fashions that optimize the timing for management . There’s an acute want for managing the downy mildew pathogens infecting cucurbits and hop incited by members of the genus Pseudoperonospora (P. cubensis clade 1 and a couple of isolates and P. humuli, respectively).

A extremely particular multiplex TaqMan qPCR assay concentrating on distinctive sequences within the pathogens’ mitochondrial genomes was developed that permits detection of all three taxa in a single multiplexed amplification. An inside management included within the response evaluated if outcomes have been influenced by PCR inhibitors that may make it by means of the DNA extraction course of.

Dependable quantification of inoculum as little as three sporangia in a pattern was noticed. The multiplexed assay was examined with DNA extracted from purified sporangia, contaminated plant tissue and environmental samples collected on impaction spore traps samplers. The flexibility to precisely detect and concurrently quantify all three pathogens in a single multiplexed amplification ought to enhance administration choices for controlling the illnesses they trigger.

Identification and validation of reference genes for dependable evaluation of differential gene expression throughout antibiotic induced persister formation in Klebsiella pneumoniae utilizing qPCR

The examine of differential gene expression in persister cells is compounded by ceasure of typical mobile metabolic pathways throughout persistence. There’s, therefore, a requirement to establish and validate appropriate reference genes whose expression stays secure throughout persistence. We evaluated the suitability of 5 genes viz. dnaJ, groEL, rpoB, kp751, kp4432 as references to check gene expression utilizing real-time polymerase chain response (qPCR) throughout persister cell formation in Klebsiella pneumoniae. Outcomes obtained confirmed that whereas dnaJ and groEL suffered from unstable expression; rpoB, kp751 and kp4432 confirmed secure expression.

Additional, it was noticed that information normalization utilizing both of the secure genes viz. rpoB, kp751, kp4432 alone, resulted in both too low expression ranges or too excessive variation amongst replicates. Our examine signifies the concurrent use of kp4432 and rpoB as reference genes to be probably the most appropriate for dependable evaluation of differential gene expression throughout antibiotic induced persister formation in Okay. pneumoniae. kp4432 and rpoB encode NAD-dependant phenylacetaldehyde dehydrogenase and DNA-directed RNA polymerase beta subunit respectively.

The result of this examine will enhance the utility of qPCR in finding out the temporal adjustments in gene expression throughout persistence. The examine can even help in understanding mechanisms underlying persister cell formation notably in Okay. pneumoniae.

Comparability of qPCR and Metabarcoding Strategies as Instruments for the Detection of Airborne Inoculum of Forest Fungal Pathogens

Forest illnesses brought on by invasive fungal pathogens have gotten extra widespread, typically with dramatic penalties to forest ecosystems. The event of early detection programs is important for environment friendly surveillance and to mitigate the impression of invasive pathogens. Windborne spores are an essential pathway for introduction of fungal pathogens into new areas; the design of spore trapping units tailored to forests, able to gathering various kinds of spores, and aligned with improvement of environment friendly molecular strategies for detection of the pathogen, ought to assist forest managers anticipate new illness outbreaks.

Two varieties of Rotorod samplers have been evaluated for the gathering of airborne inoculum of forest fungal pathogens with a variety of spore sizes in 5 forest varieties. Detection was by particular quantitative PCR (qPCR) and by high-throughput sequencing (HTS) of amplified inside transcribed spacer sequences utilizing a brand new bioinformatic pipeline, FungiSearch, developed for diagnostic functions. Validation of the pipeline was performed on mock communities of 10 fungal species belonging to totally different taxa.

Green qPCR SuperMix UDG
abx098032-100l	Abbexa	100 µl	EUR 687.5

Green qPCR SuperMix UDG
20-abx098032	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

Green qPCR SuperMix UDG
abx098032-200l	Abbexa	200 µl	EUR 912.5

Top Green qPCR SuperMix
abx098033-100l	Abbexa	100 µl	EUR 262.5

Top Green qPCR SuperMix
20-abx098033	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Top Green qPCR SuperMix
abx098033-200l	Abbexa	200 µl	EUR 487.5

Tip Green qPCR SuperMix
abx098034-100l	Abbexa	100 µl	EUR 262.5

Tip Green qPCR SuperMix
20-abx098034	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Tip Green qPCR SuperMix
abx098034-200l	Abbexa	200 µl	EUR 437.5

Library Quantification qPCR SuperMix
abx098896-100l	Abbexa	100 µl	EUR 700

Library Quantification qPCR SuperMix
abx098896-200l	Abbexa	200 µl	EUR 962.5

Library Quantification qPCR SuperMix
20-abx098896	Abbexa	Ask for price Ask for price	1 ml 5 ml

EnTurbo™ probe qPCR SuperMix
EQ017	ELK Biotech	5mL	EUR 240

EnTurbo™ probe qPCR SuperMix
EQ017-25mL	ELK Biotech	25mL	EUR 240

EnTurbo™ probe qPCR SuperMix
EQ017-5mL	ELK Biotech	5mL	EUR 78

Top Green qPCR SuperMix (+Dye II)
abx098890-100l	Abbexa	100 µl	EUR 262.5

Top Green qPCR SuperMix (+Dye II)
20-abx098890	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Top Green qPCR SuperMix (+Dye II)
abx098890-200l	Abbexa	200 µl	EUR 487.5

Tip Green qPCR SuperMix (+Dye II)
abx098891-100l	Abbexa	100 µl	EUR 262.5

Tip Green qPCR SuperMix (+Dye II)
20-abx098891	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Tip Green qPCR SuperMix (+Dye II)
abx098891-200l	Abbexa	200 µl	EUR 437.5

EnTurbo™ SYBR Color qPCR SuperMix
EQ036	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix
EQ036-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix
EQ036-5mL	ELK Biotech	5mL	EUR 80

cDNA Synthesis SuperMix for qPCR
abx098019-100l	Abbexa	100 µl	EUR 825

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns ×20 ul Systems

cDNA Synthesis SuperMix for qPCR
abx098019-1ml	Abbexa	1 ml	EUR 2387.5

cDNA Synthesis SuperMix for qPCR
abx098019-200l	Abbexa	200 µl	EUR 1000

HyperScript™ RT SuperMix for qPCR
K1074-100	ApexBio	100 rxn (20 uL/rxn)	EUR 296
Description: Reverse Transcription & PCR|Reverse Transcription

HyperScript™ RT SuperMix for qPCR
K1074-50	ApexBio	50 rxn (20 uL/rxn)	EUR 166.4
Description: Reverse Transcription & PCR|Reverse Transcription

HiScript II Q RT SuperMix for qPCR
R222-01	Vazyme	100 rxn (20 μl/rxn)	EUR 145

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035-5mL	ELK Biotech	5mL	EUR 80

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-100l	Abbexa	100 µl	EUR 1025

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-1ml	Abbexa	1 ml	Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-200l	Abbexa	200 µl	Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 861.6

HiScript II Q Select RT SuperMix for qPCR
R232-01	Vazyme	100 rxn (20 μl/rxn)	EUR 145

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-01	Vazyme	100rxns(20µl/rxn)	EUR 326

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-01-100rxns	Vazyme	100 rxns	EUR 337.36

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-100l	Abbexa	100 µl	EUR 1012.5

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-1ml	Abbexa	1 ml	Ask for price

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-200l	Abbexa	200 µl	Ask for price

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-01	Vazyme	100 rxn (20 μl/rxn)	EUR 217.5

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-01-100rxns	Vazyme	100 rxns	EUR 225.22

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034-5mL	ELK Biotech	5mL	EUR 80

HiScript II Q RT SuperMix for qPCR (+gDNA wiper)
R223-01	Vazyme	100rxns(20µl/rxn)	EUR 159.5

HyperScript™ RT SuperMix for qPCR (with gDNA wiper)
K1174-100	ApexBio	100 rxns	EUR 320
Description: Reverse Transcription & PCR|Reverse Transcription

HyperScript™ RT SuperMix for qPCR (with gDNA wiper)
K1174-50	ApexBio	50 rxns	EUR 192
Description: Reverse Transcription & PCR|Reverse Transcription

HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper)
R233-01	Vazyme	100 rxn (20 μl/rxn)	EUR 159.5

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01	Vazyme	100 rxns (21 μl/rxn)	EUR 217.5

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01-100rxns	Vazyme	100 rxns	EUR 225.22

Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus)
11141ES10	Yeasen Biotechnology	10T	EUR 25

Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus)
11141ES60	Yeasen Biotechnology	100T	EUR 230

PCR SuperMix
20-abx098887	Abbexa	Ask for price Ask for price	1 ml 5 ml

Eva-QPCR-SuperMix-Kit
K5052200	Biochain	200 reactions	EUR 147.7

Eva-QPCR-SuperMix-Kit
K5052400	Biochain	400 reactions	EUR 247.8

2X PCR SuperMix
MB200-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

2X PCR SuperMix
MBS402025-100Reactions	MyBiosource	100Reactions	EUR 175

2X PCR SuperMix
MBS402025-4Reactions	MyBiosource	4Reactions	EUR 160

PCR SuperMix (-dye)
abx098002-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix (-dye)
20-abx098002	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

PCR SuperMix (-dye)
abx098002-200l	Abbexa	200 µl	EUR 337.5

PCR SuperMix (+dye)
abx098003-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix (+dye)
20-abx098003	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 480 ml

PCR SuperMix (+dye)
abx098003-200l	Abbexa	200 µl	EUR 337.5

Plus PCR SuperMix
abx098888-100l	Abbexa	100 µl	EUR 300

Plus PCR SuperMix
abx098888-200l	Abbexa	200 µl	EUR 612.5

Plus PCR SuperMix
20-abx098888	Abbexa	Ask for price Ask for price	1 ml 5 ml

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

Probe Direct SuperMix
abx461057-1096tests	Abbexa	10 × 96 tests	Ask for price

Probe Direct SuperMix
abx461057-596tests	Abbexa	5 × 96 tests	EUR 2800

Probe Direct SuperMix
abx461057-96tests	Abbexa	96 tests	EUR 812.5

cDNA Synthesis SuperMix
abx098014-100l	Abbexa	100 µl	EUR 725

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

cDNA Synthesis SuperMix
abx098014-1ml	Abbexa	1 ml	Ask for price

cDNA Synthesis SuperMix
abx098014-200l	Abbexa	200 µl	EUR 812.5

T PCR SuperMix (-dye)
abx098005-100l	Abbexa	100 µl	EUR 262.5

T PCR SuperMix (-dye)
abx098005-200l	Abbexa	200 µl	EUR 462.5

T PCR SuperMix (-dye)
20-abx098005	Abbexa	Ask for price Ask for price	1 ml 5 ml

T PCR SuperMix (+dye)
abx098006-100l	Abbexa	100 µl	EUR 262.5

T PCR SuperMix (+dye)
abx098006-200l	Abbexa	200 µl	EUR 462.5

T PCR SuperMix (+dye)
20-abx098006	Abbexa	Ask for price Ask for price	1 ml 5 ml

AmpHS 2X PCR SuperMix
MB203-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

EasyPfu PCR SuperMix (-dye)
20-abx098009	Abbexa	Ask for price Ask for price	1 ml 5 ml

FastPfu PCR SuperMix (-dye)
abx098010-100l	Abbexa	100 µl	EUR 300

FastPfu PCR SuperMix (-dye)
abx098010-200l	Abbexa	200 µl	EUR 575

FastPfu PCR SuperMix (-dye)
20-abx098010	Abbexa	Ask for price Ask for price	1 ml 5 ml

Library Amplification SuperMix
abx098894-100l	Abbexa	100 µl	EUR 737.5

Library Amplification SuperMix
abx098894-200l	Abbexa	200 µl	EUR 1137.5

Library Amplification SuperMix
20-abx098894	Abbexa	Ask for price Ask for price	1 ml 5 ml

OneScriptcDNA Synthesis SuperMix
G451	ABM	25 x 20 ul reactions	EUR 106.8

OneScriptcDNA Synthesis SuperMix
G452	ABM	100 x 20 ul reactions	EUR 169.2

PCR SuperMix for PAGE (+dye)
abx098004-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix for PAGE (+dye)
20-abx098004	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

PCR SuperMix for PAGE (+dye)
abx098004-200l	Abbexa	200 µl	EUR 337.5

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 202.8

OmniPCR Supermix w Fluorescent dye
MBA01-0100	Bio-Helix	100 rxns (2.5ml)	EUR 16

EnTurbo™ SYBR Green PCR SuperMix
EQ001	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix
EQ001-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix
EQ001-5mL	ELK Biotech	5mL	EUR 80

Green Two-Step qRT-PCR SuperMix
abx098035-100l	Abbexa	100 µl	EUR 925

Green Two-Step qRT-PCR SuperMix
abx098035-1ml	Abbexa	1 ml	Ask for price

Green Two-Step qRT-PCR SuperMix
abx098035-200l	Abbexa	200 µl	Ask for price

Green Two-Step qRT-PCR SuperMix
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 777.6

Green One-Step qRT-PCR SuperMix
abx098038-100l	Abbexa	100 µl	EUR 737.5

Green One-Step qRT-PCR SuperMix
20-abx09803820ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Green One-Step qRT-PCR SuperMix
abx098038-1ml	Abbexa	1 ml	Ask for price

Green One-Step qRT-PCR SuperMix
abx098038-200l	Abbexa	200 µl	EUR 1050

Probe One-Step qRT-PCR SuperMix
abx098042-100l	Abbexa	100 µl	EUR 850

Probe One-Step qRT-PCR SuperMix
20-abx09804220ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix
abx098042-1ml	Abbexa	1 ml	Ask for price

Probe One-Step qRT-PCR SuperMix
abx098042-200l	Abbexa	200 µl	EUR 1400

AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X)
MB204-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 13

Two-Step RT-PCR SuperMix (12 kb)
abx098025-100l	Abbexa	100 µl	EUR 887.5

Two-Step RT-PCR SuperMix (12 kb)
abx098025-1ml	Abbexa	1 ml	Ask for price

Two-Step RT-PCR SuperMix (12 kb)
abx098025-200l	Abbexa	200 µl	Ask for price

Two-Step RT-PCR SuperMix (12 kb)
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)
abx098026-100l	Abbexa	100 µl	EUR 1087.5

Two-Step RT-PCR SuperMix (15 kb)
abx098026-1ml	Abbexa	1 ml	Ask for price

Two-Step RT-PCR SuperMix (15 kb)
abx098026-200l	Abbexa	200 µl	Ask for price

Two-Step RT-PCR SuperMix (15 kb)
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 910.8

All-in-One cDNA Synthesis SuperMix
B24403	Bimake	200 ractions	EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408	Bimake	1000 ractions	EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

OmniPCR HS Supermix w Fluorescent dye
MBA03-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X)
MB202-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

OmniPCR FAST Supermix w Fluorescent dye
MBA02-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

OmniPCR Long Supermix w Fluorescent dye
MBA04-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

OnePCR Ultra Supermix w Fluorescent Dye
MBS402026-100Reactions	MyBiosource	100Reactions	EUR 180

OnePCR Ultra Supermix w Fluorescent Dye
MBS402026-4Reactions	MyBiosource	4Reactions	EUR 160

Fly First-Strand cDNA Synthesis SuperMix
abx098858-100l	Abbexa	100 µl	EUR 850

Fly First-Strand cDNA Synthesis SuperMix
20-abx09885820ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

Fly First-Strand cDNA Synthesis SuperMix
abx098858-1ml	Abbexa	1 ml	Ask for price

Fly First-Strand cDNA Synthesis SuperMix
abx098858-200l	Abbexa	200 µl	EUR 1037.5

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-100l	Abbexa	100 µl	EUR 875

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200l	Abbexa	200 µl	Ask for price

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-100l	Abbexa	100 µl	EUR 950

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200l	Abbexa	200 µl	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-100l	Abbexa	100 µl	EUR 1212.5

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200l	Abbexa	200 µl	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 1028.4

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-100l	Abbexa	100 µl	EUR 1212.5

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-1ml	Abbexa	1 ml	Ask for price

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-200l	Abbexa	200 µl	Ask for price

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems	Abbexa	20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)	EUR 1062

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-100l	Abbexa	100 µl	EUR 950

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-1ml	Abbexa	1 ml	Ask for price

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-200l	Abbexa	200 µl	Ask for price

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-20rxns20ulSystems	Abbexa	20 rxns × 20 ul Systems	EUR 878.4

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
abx098007-100l	Abbexa	100 µl	EUR 262.5

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
abx098007-200l	Abbexa	200 µl	EUR 475

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
20-abx098007	Abbexa	Ask for price Ask for price	1 ml 5 ml

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-100l	Abbexa	100 µl	EUR 1125

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-1ml	Abbexa	1 ml	Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-200l	Abbexa	200 µl	Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 961.2

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-100l	Abbexa	100 µl	EUR 450

Green One-Step qRT-PCR SuperMix (GC Rich)
20-abx09803920ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-1ml	Abbexa	1 ml	Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-200l	Abbexa	200 µl	EUR 1000

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-100l	Abbexa	100 µl	EUR 737.5

Probe One-Step qRT-PCR SuperMix (GC Rich)
20-abx09804120ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-1ml	Abbexa	1 ml	Ask for price

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-200l	Abbexa	200 µl	EUR 1050

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-100l	Abbexa	100 µl	EUR 850

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-1ml	Abbexa	1 ml	Ask for price

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-200l	Abbexa	200 µl	Ask for price

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 693.6

HyperScript™ First-Strand cDNA Synthesis SuperMix
K1073-100	ApexBio	100 rxn (20 uL/rxn)	EUR 297.6
Description: Reverse Transcription & PCR|Reverse Transcription

HyperScript™ First-Strand cDNA Synthesis SuperMix
K1073-50	ApexBio	50 rxn (20 uL/rxn)	EUR 166.4
Description: Reverse Transcription & PCR|Reverse Transcription

One-Step gDNA Removal and cDNA Synthesis SuperMix
abx098859-100l	Abbexa	100 µl	EUR 1062.5

One-Step gDNA Removal and cDNA Synthesis SuperMix
20-abx09885920ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix
abx098859-1ml	Abbexa	1 ml	Ask for price

One-Step gDNA Removal and cDNA Synthesis SuperMix
abx098859-200l	Abbexa	200 µl	EUR 1425

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013-5mL	ELK Biotech	5mL	EUR 80

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
abx098022-100l	Abbexa	100 µl	EUR 1012.5

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
20-abx09802220ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
abx098022-1ml	Abbexa	1 ml	Ask for price

Though the sensitivity of the brand new HTS pipeline was decrease than the particular qPCR, it was in a position to detect all kinds of fungal pathogens. FungiSearch is simple to make use of, and the reference database is updatable, making the device appropriate for speedy identification of recent pathogens. This new strategy combining spore trapping and HTS detection is promising as a diagnostic device for invasive fungal pathogens.